Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID
Summary: The majority of fluorescent vessel labeling techniques currently available are limited by their expense, incomplete labeling, or complexity. Here, we present VALID (vessel labeling via gelatin-based lipophilic dye solution)—a protocol for complete labeling of different vascular networks. We...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004082 |
| _version_ | 1797752479593529344 |
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| author | Jingtan Zhu Xiaomei Liu Jianyi Xu Zhang Liu Yating Deng Junyao Dai Tingting Yu Dan Zhu |
| author_facet | Jingtan Zhu Xiaomei Liu Jianyi Xu Zhang Liu Yating Deng Junyao Dai Tingting Yu Dan Zhu |
| author_sort | Jingtan Zhu |
| collection | DOAJ |
| description | Summary: The majority of fluorescent vessel labeling techniques currently available are limited by their expense, incomplete labeling, or complexity. Here, we present VALID (vessel labeling via gelatin-based lipophilic dye solution)—a protocol for complete labeling of different vascular networks. We describe steps for preparing different dye hydrogels, murine vascular casting and tissue harvesting, immunolabeling, tissue clearing, and imaging, as well as detailed analysis of the vascular networks. This protocol is helpful for evaluating vascular lesions in studying different vessel-associated diseases.For complete details on the use and execution of this protocol, please refer to Zhu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T17:04:01Z |
| format | Article |
| id | doaj.art-6e1afccdef924eae83645d59700a1d1a |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T17:04:01Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-6e1afccdef924eae83645d59700a1d1a2023-08-07T04:04:57ZengElsevierSTAR Protocols2666-16672023-09-0143102441Protocol for fine casting, imaging, and analysis of murine vascular networks with VALIDJingtan Zhu0Xiaomei Liu1Jianyi Xu2Zhang Liu3Yating Deng4Junyao Dai5Tingting Yu6Dan Zhu7Britton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, ChinaBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China; Corresponding authorBritton Chance Center for Biomedical Photonics - MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China; Corresponding authorSummary: The majority of fluorescent vessel labeling techniques currently available are limited by their expense, incomplete labeling, or complexity. Here, we present VALID (vessel labeling via gelatin-based lipophilic dye solution)—a protocol for complete labeling of different vascular networks. We describe steps for preparing different dye hydrogels, murine vascular casting and tissue harvesting, immunolabeling, tissue clearing, and imaging, as well as detailed analysis of the vascular networks. This protocol is helpful for evaluating vascular lesions in studying different vessel-associated diseases.For complete details on the use and execution of this protocol, please refer to Zhu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004082MicroscopyMolecular/Chemical ProbesBiotechnology and bioengineering |
| spellingShingle | Jingtan Zhu Xiaomei Liu Jianyi Xu Zhang Liu Yating Deng Junyao Dai Tingting Yu Dan Zhu Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID STAR Protocols Microscopy Molecular/Chemical Probes Biotechnology and bioengineering |
| title | Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID |
| title_full | Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID |
| title_fullStr | Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID |
| title_full_unstemmed | Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID |
| title_short | Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID |
| title_sort | protocol for fine casting imaging and analysis of murine vascular networks with valid |
| topic | Microscopy Molecular/Chemical Probes Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004082 |
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