Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID
Summary: The majority of fluorescent vessel labeling techniques currently available are limited by their expense, incomplete labeling, or complexity. Here, we present VALID (vessel labeling via gelatin-based lipophilic dye solution)—a protocol for complete labeling of different vascular networks. We...
Main Authors: | Jingtan Zhu, Xiaomei Liu, Jianyi Xu, Zhang Liu, Yating Deng, Junyao Dai, Tingting Yu, Dan Zhu |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2023-09-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004082 |
Similar Items
-
A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution
by: Jingtan Zhu, et al.
Published: (2023-02-01) -
Protocol for wireless deep brain stimulation in freely behaving mice with infrared light
by: Xiang Wu, et al.
Published: (2023-03-01) -
Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models
by: Adele Moatti, et al.
Published: (2023-06-01) -
Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
by: Yue Guo, et al.
Published: (2022-09-01) -
Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening
by: Lea Peko, et al.
Published: (2024-03-01)