A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice
Cynatratoside A (CyA) is a C21 Steroidal glycoside with pregnane skeleton isolated from the root of Cynanchum atratum Bunge (Asclepiadaceae). This study aimed to investigate the effects of CyA on concanavalin A (Con A)-induced autoimmune hepatitis (AIH) and the underlying mechanism. CyA was orally a...
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MDPI AG
2019-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/6/1087 |
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| author | Jian Yang Bin Wang Chao-feng Zhang Xiang-hong Xu Mian Zhang |
| author_facet | Jian Yang Bin Wang Chao-feng Zhang Xiang-hong Xu Mian Zhang |
| author_sort | Jian Yang |
| collection | DOAJ |
| description | Cynatratoside A (CyA) is a C21 Steroidal glycoside with pregnane skeleton isolated from the root of Cynanchum atratum Bunge (Asclepiadaceae). This study aimed to investigate the effects of CyA on concanavalin A (Con A)-induced autoimmune hepatitis (AIH) and the underlying mechanism. CyA was orally administered to mice at 10 and 40 mg/kg 8 h before and 1 h after Con A treatment. The effects of CyA on Con A-induced spleen and liver in mice were assessed via histopathological changes, T lymphocyte amounts and the expressions of IL-1β and ICAM-1. Con A-induced L-02 hepatocytes were used to evaluate whether CyA (0.1–10 μM) can directly protect hepatocytes from cytotoxicity and the possible mechanism. The results revealed that CyA treatment could significantly improve the histopathological changes of spleen and liver, reduce the proliferation of splenic T lymphocytes, and decrease the expressions of IL-1β and ICAM-1 in liver. The experiment in vitro showed that CyA inhibited Con A-induced hepatotoxicity in a concentration-dependent manner. CyA (10 μM) significantly increased/decreased the expression of Bcl-2/Bax and reduced the levels of cleaved caspases-9 and -3. Our study demonstrated for the first time that CyA has a significant protective effect on Con A-induced AIH by inhibiting the activation and adhesion of T lymphocytes and blocking hepatocyte apoptosis. |
| first_indexed | 2024-12-20T06:31:43Z |
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| id | doaj.art-6e1c70cb37ef4b269ca8ca499c217604 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T06:31:43Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6e1c70cb37ef4b269ca8ca499c2176042022-12-21T19:50:08ZengMDPI AGMolecules1420-30492019-03-01246108710.3390/molecules24061087molecules24061087A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in MiceJian Yang0Bin Wang1Chao-feng Zhang2Xiang-hong Xu3Mian Zhang4School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaSchool of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaSchool of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaSchool of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaSchool of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaCynatratoside A (CyA) is a C21 Steroidal glycoside with pregnane skeleton isolated from the root of Cynanchum atratum Bunge (Asclepiadaceae). This study aimed to investigate the effects of CyA on concanavalin A (Con A)-induced autoimmune hepatitis (AIH) and the underlying mechanism. CyA was orally administered to mice at 10 and 40 mg/kg 8 h before and 1 h after Con A treatment. The effects of CyA on Con A-induced spleen and liver in mice were assessed via histopathological changes, T lymphocyte amounts and the expressions of IL-1β and ICAM-1. Con A-induced L-02 hepatocytes were used to evaluate whether CyA (0.1–10 μM) can directly protect hepatocytes from cytotoxicity and the possible mechanism. The results revealed that CyA treatment could significantly improve the histopathological changes of spleen and liver, reduce the proliferation of splenic T lymphocytes, and decrease the expressions of IL-1β and ICAM-1 in liver. The experiment in vitro showed that CyA inhibited Con A-induced hepatotoxicity in a concentration-dependent manner. CyA (10 μM) significantly increased/decreased the expression of Bcl-2/Bax and reduced the levels of cleaved caspases-9 and -3. Our study demonstrated for the first time that CyA has a significant protective effect on Con A-induced AIH by inhibiting the activation and adhesion of T lymphocytes and blocking hepatocyte apoptosis.http://www.mdpi.com/1420-3049/24/6/1087Cynatratoside Aautoimmune hepatitisspleenT lymphocyteapoptosisCynanchum atratum |
| spellingShingle | Jian Yang Bin Wang Chao-feng Zhang Xiang-hong Xu Mian Zhang A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice Molecules Cynatratoside A autoimmune hepatitis spleen T lymphocyte apoptosis Cynanchum atratum |
| title | A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice |
| title_full | A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice |
| title_fullStr | A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice |
| title_full_unstemmed | A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice |
| title_short | A C21-Steroidal Glycoside from Cynanchum atratum Attenuates Concanavalin A-Induced Liver Injury in Mice |
| title_sort | c21 steroidal glycoside from cynanchum atratum attenuates concanavalin a induced liver injury in mice |
| topic | Cynatratoside A autoimmune hepatitis spleen T lymphocyte apoptosis Cynanchum atratum |
| url | http://www.mdpi.com/1420-3049/24/6/1087 |
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