| Summary: | The purpose of the research was to develop and study biocatalysts based on ficin associates with chitosan nanoparticles. We obtained medium and high molecular weight chitosan nanoparticles with the addition of ascorbic acid and without it.The zeta potential of all types of nanoparticles was 0 mV. The associates of ficin and chitosan nanoparticles formed with the addition of ascorbic acid exhibited higher proteolytic activity. While determining the stability of the associates of chitosan and ficin nanoparticles, we noticed a decrease in the proteolytic activity of the samples within seven days. Medium and high molecular weight chitosan nanoparticles obtained using ascorbic acid differed significantly in size from the nanoparticles produced without ascorbic acid. The proposed biocatalysts have high prospects for use in cosmetology,
biomedicine, and pharmacy.
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