The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca
Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the m...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2007-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-4-0430 |
| _version_ | 1811291267064135680 |
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| author | Diego Romero Antonio de Vicente Rivo H. Rakotoaly Samuel E. Dufour Jan-Willem Veening Eva Arrebola Francisco M. Cazorla Oscar P. Kuipers Michel Paquot Alejandro Pérez-García |
| author_facet | Diego Romero Antonio de Vicente Rivo H. Rakotoaly Samuel E. Dufour Jan-Willem Veening Eva Arrebola Francisco M. Cazorla Oscar P. Kuipers Michel Paquot Alejandro Pérez-García |
| author_sort | Diego Romero |
| collection | DOAJ |
| description | Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopep-tides have a major role in the antagonism of B. subtilis toward P. fusca. |
| first_indexed | 2024-04-13T04:26:52Z |
| format | Article |
| id | doaj.art-6e1f7cf4d8244298a5739fe25546f218 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:52Z |
| publishDate | 2007-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-6e1f7cf4d8244298a5739fe25546f2182022-12-22T03:02:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-04-0120443044010.1094/MPMI-20-4-0430The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fuscaDiego RomeroAntonio de VicenteRivo H. RakotoalySamuel E. DufourJan-Willem VeeningEva ArrebolaFrancisco M. CazorlaOscar P. KuipersMichel PaquotAlejandro Pérez-GarcíaPodosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol performance. Cell-free supernatants showed antifungal activities very close to those previously reported for vegetative cells. Identification of three lipopeptide antibiotics, surfactin, fengycin, and iturin A or bacillomycin, in butanolic extracts from cell-free culture filtrates of these B. subtilis strains pointed out that antibiosis could be a major factor involved in their biocontrol ability. The strong inhibitory effect of purified lipopeptide fractions corresponding to bacillomycin, fengycin, and iturin A on P. fusca conidia germination, as well as the in situ detection of these lipopeptides in bacterial-treated melon leaves, provided interesting evidence of their putative involvement in the antagonistic activity. Those results were definitively supported by site-directed mutagenesis analysis, targeted to suppress the biosynthesis of the different lipopeptides. Taken together, our data have allowed us to conclude that the iturin and fengycin families of lipopep-tides have a major role in the antagonism of B. subtilis toward P. fusca.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-4-0430antifungalsbiological controlcucurbits |
| spellingShingle | Diego Romero Antonio de Vicente Rivo H. Rakotoaly Samuel E. Dufour Jan-Willem Veening Eva Arrebola Francisco M. Cazorla Oscar P. Kuipers Michel Paquot Alejandro Pérez-García The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca Molecular Plant-Microbe Interactions antifungals biological control cucurbits |
| title | The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca |
| title_full | The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca |
| title_fullStr | The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca |
| title_full_unstemmed | The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca |
| title_short | The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis Toward Podosphaera fusca |
| title_sort | iturin and fengycin families of lipopeptides are key factors in antagonism of bacillus subtilis toward podosphaera fusca |
| topic | antifungals biological control cucurbits |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-4-0430 |
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