Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea
Fusarium wilt and Fusarium surface rot caused by <i>Fusarium oxysporum</i> Schltdl are the major diseases of sweetpotato (<i>Ipomoea batatas</i>) and was surveyed in different locations (Cheongju, Heanam, Iksan, Icheon, Kimje, Nonsan, Yeoungam, and Yeoju) in Korea from 2015 t...
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MDPI AG
2020-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/4/497 |
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| author | Narayan Chandra Paul Won Park Seungyong Lee Mi Nam Chung Hyeong-Un Lee Jung-Wook Yang |
| author_facet | Narayan Chandra Paul Won Park Seungyong Lee Mi Nam Chung Hyeong-Un Lee Jung-Wook Yang |
| author_sort | Narayan Chandra Paul |
| collection | DOAJ |
| description | Fusarium wilt and Fusarium surface rot caused by <i>Fusarium oxysporum</i> Schltdl are the major diseases of sweetpotato (<i>Ipomoea batatas</i>) and was surveyed in different locations (Cheongju, Heanam, Iksan, Icheon, Kimje, Nonsan, Yeoungam, and Yeoju) in Korea from 2015 to 2017 in the field, after harvesting and in storehouse. The wilt incidence in the early stage represented 17.9%, 5.9%, and 8.3% in 2015, 2016, and 2017, respectively. Samples were collected, and the causal organism was isolated on potato dextrose agar (PDA). Ten pure cultures were stored at the Sweetpotato Research Laboratory, Bioenergy Crop Research Institute, Muan, Korea. Morphological analysis, along with molecular phylogeny of the sequences of internal-transcribed spacer (ITS) and elongation factor 1-α (EF-1α) genes and their combined phylogenetic analysis, confirmed the isolates as the <i>Fusarium oxysporum</i> Schltdl. Pathogenicity tests were conducted on sweetpotato stems, and storage roots by artificially inoculation methods, and the most virulent isolate was selected as SPL18019. A rapid screening method on 21 selected varieties for resistant variety selection was applied on stems. The Pungwanmi was found resistant to Fusarium wilt, whereas Annobeni was the most susceptible. On the other hand, six varieties were used to test surface rot resistance, and Yulmi and Yesumi were resistant and susceptible, respectively, to Fusarium surface rot. |
| first_indexed | 2024-03-10T20:30:05Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:30:05Z |
| publishDate | 2020-04-01 |
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| series | Plants |
| spelling | doaj.art-6e232681a66245b9a717f06352c730ca2023-11-19T21:29:06ZengMDPI AGPlants2223-77472020-04-019449710.3390/plants9040497Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in KoreaNarayan Chandra Paul0Won Park1Seungyong Lee2Mi Nam Chung3Hyeong-Un Lee4Jung-Wook Yang5Bioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, KoreaBioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, KoreaBioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, KoreaBioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, KoreaBioenergy Crop Research Institute, National Institute of Crop Science, RDA, Muan 58545, KoreaCrop Cultivation and Environment Research Division, National Institute of Crop Science, RDA, Suwon 16613, KoreaFusarium wilt and Fusarium surface rot caused by <i>Fusarium oxysporum</i> Schltdl are the major diseases of sweetpotato (<i>Ipomoea batatas</i>) and was surveyed in different locations (Cheongju, Heanam, Iksan, Icheon, Kimje, Nonsan, Yeoungam, and Yeoju) in Korea from 2015 to 2017 in the field, after harvesting and in storehouse. The wilt incidence in the early stage represented 17.9%, 5.9%, and 8.3% in 2015, 2016, and 2017, respectively. Samples were collected, and the causal organism was isolated on potato dextrose agar (PDA). Ten pure cultures were stored at the Sweetpotato Research Laboratory, Bioenergy Crop Research Institute, Muan, Korea. Morphological analysis, along with molecular phylogeny of the sequences of internal-transcribed spacer (ITS) and elongation factor 1-α (EF-1α) genes and their combined phylogenetic analysis, confirmed the isolates as the <i>Fusarium oxysporum</i> Schltdl. Pathogenicity tests were conducted on sweetpotato stems, and storage roots by artificially inoculation methods, and the most virulent isolate was selected as SPL18019. A rapid screening method on 21 selected varieties for resistant variety selection was applied on stems. The Pungwanmi was found resistant to Fusarium wilt, whereas Annobeni was the most susceptible. On the other hand, six varieties were used to test surface rot resistance, and Yulmi and Yesumi were resistant and susceptible, respectively, to Fusarium surface rot.https://www.mdpi.com/2223-7747/9/4/497Fusarium wilt<i>Fusarium oxysporum</i>molecular phylogenysurface rotsweetpotato |
| spellingShingle | Narayan Chandra Paul Won Park Seungyong Lee Mi Nam Chung Hyeong-Un Lee Jung-Wook Yang Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea Plants Fusarium wilt <i>Fusarium oxysporum</i> molecular phylogeny surface rot sweetpotato |
| title | Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea |
| title_full | Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea |
| title_fullStr | Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea |
| title_full_unstemmed | Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea |
| title_short | Occurrence of Sweetpotato (<i>Ipomoea batatas</i>) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea |
| title_sort | occurrence of sweetpotato i ipomoea batatas i wilt and surface rot disease and determining resistance of selected varieties to the pathogen in korea |
| topic | Fusarium wilt <i>Fusarium oxysporum</i> molecular phylogeny surface rot sweetpotato |
| url | https://www.mdpi.com/2223-7747/9/4/497 |
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