Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales)
Samsoniella is a ubiquitous genus of cosmopolitan arthropod-pathogenic fungi in the family Cordycipitaceae. The fungi have economic, medicinal, and ecological importance. Prior taxonomic studies of these fungi relied predominantly on phylogenetic inferences from five loci, namely, the nuclear riboso...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2023-10-01
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| Series: | MycoKeys |
| Online Access: | https://mycokeys.pensoft.net/article/106474/download/pdf/ |
| _version_ | 1797665944799019008 |
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| author | Yao Wang Zhi-Qin Wang Chinnapan Thanarut Van-Minh Dao Yuan-Bing Wang Hong Yu |
| author_facet | Yao Wang Zhi-Qin Wang Chinnapan Thanarut Van-Minh Dao Yuan-Bing Wang Hong Yu |
| author_sort | Yao Wang |
| collection | DOAJ |
| description | Samsoniella is a ubiquitous genus of cosmopolitan arthropod-pathogenic fungi in the family Cordycipitaceae. The fungi have economic, medicinal, and ecological importance. Prior taxonomic studies of these fungi relied predominantly on phylogenetic inferences from five loci, namely, the nuclear ribosomal small and large subunits (nr SSU and nr LSU), the 3’ portion of translation elongation factor 1 alpha (3P_TEF), and RNA polymerase II subunits 1 and 2 (RPB1 and RPB2). Despite many new species being described, not all of the recognized species inside this group formed well-supported clades. Thus, the search for new markers appropriate for molecular phylogenetic analysis of Samsoniella remains a challenging problem. In our study, we selected the internal transcribed spacer regions of the rDNA (ITS rDNA) and seven gene regions, namely, 3P_TEF, the 5’ portion of translation elongation factor 1 alpha (5P_TEF), RPB1, RPB2, γ-actin (ACT), β-tubulin (TUB), and a gene encoding a minichromosome maintenance protein (MCM7), as candidate markers for species identification. Genetic divergence comparisons showed that the ITS, RPB2, ACT, and TUB sequences provided little valuable information with which to separate Samsoniella spp. In contrast, sequence data for 3P_TEF, 5P_TEF, RPB1, and MCM7 provided good resolution of Samsoniella species. The phylogenetic tree inferred from combined data (5P_TEF + 3P_TEF + RPB1 + MCM7) showed well-supported clades for Samsoniella and allowed for the delimitation of 26 species in this genus. The other two species (S. formicae and S. lepidopterorum) were not evaluated, as they had abundant missing data. |
| first_indexed | 2024-03-11T19:51:21Z |
| format | Article |
| id | doaj.art-6e2383199f014423868cf8d01fa4008a |
| institution | Directory Open Access Journal |
| issn | 1314-4049 |
| language | English |
| last_indexed | 2024-03-11T19:51:21Z |
| publishDate | 2023-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | MycoKeys |
| spelling | doaj.art-6e2383199f014423868cf8d01fa4008a2023-10-05T08:11:04ZengPensoft PublishersMycoKeys1314-40492023-10-019922725010.3897/mycokeys.99.106474106474Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales)Yao Wang0Zhi-Qin Wang1Chinnapan Thanarut2Van-Minh Dao3Yuan-Bing Wang4Hong Yu5Yunnan UniversityYunnan UniversityMaejo UniversityInstitute of Regional Research and Development, Ministry of Science and TechnologyYunnan UniversityYunnan UniversitySamsoniella is a ubiquitous genus of cosmopolitan arthropod-pathogenic fungi in the family Cordycipitaceae. The fungi have economic, medicinal, and ecological importance. Prior taxonomic studies of these fungi relied predominantly on phylogenetic inferences from five loci, namely, the nuclear ribosomal small and large subunits (nr SSU and nr LSU), the 3’ portion of translation elongation factor 1 alpha (3P_TEF), and RNA polymerase II subunits 1 and 2 (RPB1 and RPB2). Despite many new species being described, not all of the recognized species inside this group formed well-supported clades. Thus, the search for new markers appropriate for molecular phylogenetic analysis of Samsoniella remains a challenging problem. In our study, we selected the internal transcribed spacer regions of the rDNA (ITS rDNA) and seven gene regions, namely, 3P_TEF, the 5’ portion of translation elongation factor 1 alpha (5P_TEF), RPB1, RPB2, γ-actin (ACT), β-tubulin (TUB), and a gene encoding a minichromosome maintenance protein (MCM7), as candidate markers for species identification. Genetic divergence comparisons showed that the ITS, RPB2, ACT, and TUB sequences provided little valuable information with which to separate Samsoniella spp. In contrast, sequence data for 3P_TEF, 5P_TEF, RPB1, and MCM7 provided good resolution of Samsoniella species. The phylogenetic tree inferred from combined data (5P_TEF + 3P_TEF + RPB1 + MCM7) showed well-supported clades for Samsoniella and allowed for the delimitation of 26 species in this genus. The other two species (S. formicae and S. lepidopterorum) were not evaluated, as they had abundant missing data.https://mycokeys.pensoft.net/article/106474/download/pdf/ |
| spellingShingle | Yao Wang Zhi-Qin Wang Chinnapan Thanarut Van-Minh Dao Yuan-Bing Wang Hong Yu Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) MycoKeys |
| title | Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) |
| title_full | Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) |
| title_fullStr | Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) |
| title_full_unstemmed | Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) |
| title_short | Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) |
| title_sort | phylogeny and species delimitations in the economically medically and ecologically important genus samsoniella cordycipitaceae hypocreales |
| url | https://mycokeys.pensoft.net/article/106474/download/pdf/ |
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