PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD
An efficient protocol was established for in vitro clonal propagation for Rhizophora annamalayana Kathir. the only endemic mangrove species to India. Initially the explants were surface sterilized appropriately with 0.1% mercuric chloride for one minute, 70% ethanol for 30 seconds and 3% hydrogen pe...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Alexandru Ioan Cuza University of Iasi
2013-12-01
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| Series: | Journal of Plant Development |
| Subjects: | |
| Online Access: | http://www.plant-journal.uaic.ro/docs/2013/7.pdf |
| _version_ | 1818081550085914624 |
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| author | KATHIRESAN Kandasamy RAVINDER Singh Chinnappan |
| author_facet | KATHIRESAN Kandasamy RAVINDER Singh Chinnappan |
| author_sort | KATHIRESAN Kandasamy |
| collection | DOAJ |
| description | An efficient protocol was established for in vitro clonal propagation for Rhizophora annamalayana Kathir. the only endemic mangrove species to India. Initially the explants were surface sterilized appropriately with 0.1% mercuric chloride for one minute, 70% ethanol for 30 seconds and 3% hydrogen peroxide for one minute. Then the explants were treated with three different antioxidants for reduction of phenol browning of the explants. Among the antioxidants, 10 mg/L ascorbic acid was found to be the best. Among the five different tissue culture media - B5, WPM, MS, SH and Y3- tested, MS medium was chosen to be the best for meristem culture. Among the growth regulators, cytokinins (benzyl adinine, kinetin and zeatin) used alone and in combination with auxins (naphthalene acetic acid, indole acetic acid, indole butyric acid), the shoot growth was better observed after 20 days when MS medium was incorporated with 3.0 mg/L of benzyl adinine and 3.0 mg/L of kinetin with coconut milk. This is the initial step of tissue culture for the recovery of the fast disappearing Rhizophora annamalayana Kathir. Further research is progressing on mass multiplication and field transfer. |
| first_indexed | 2024-12-10T19:07:59Z |
| format | Article |
| id | doaj.art-6e2605c31f6f423ab76c4b2e4fbdeecd |
| institution | Directory Open Access Journal |
| issn | 2065-3158 2066-9917 |
| language | English |
| last_indexed | 2024-12-10T19:07:59Z |
| publishDate | 2013-12-01 |
| publisher | Alexandru Ioan Cuza University of Iasi |
| record_format | Article |
| series | Journal of Plant Development |
| spelling | doaj.art-6e2605c31f6f423ab76c4b2e4fbdeecd2022-12-22T01:36:51ZengAlexandru Ioan Cuza University of IasiJournal of Plant Development2065-31582066-99172013-12-012015761PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHODKATHIRESAN Kandasamy0RAVINDER Singh Chinnappan1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502 – IndiaCentre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502 – IndiaAn efficient protocol was established for in vitro clonal propagation for Rhizophora annamalayana Kathir. the only endemic mangrove species to India. Initially the explants were surface sterilized appropriately with 0.1% mercuric chloride for one minute, 70% ethanol for 30 seconds and 3% hydrogen peroxide for one minute. Then the explants were treated with three different antioxidants for reduction of phenol browning of the explants. Among the antioxidants, 10 mg/L ascorbic acid was found to be the best. Among the five different tissue culture media - B5, WPM, MS, SH and Y3- tested, MS medium was chosen to be the best for meristem culture. Among the growth regulators, cytokinins (benzyl adinine, kinetin and zeatin) used alone and in combination with auxins (naphthalene acetic acid, indole acetic acid, indole butyric acid), the shoot growth was better observed after 20 days when MS medium was incorporated with 3.0 mg/L of benzyl adinine and 3.0 mg/L of kinetin with coconut milk. This is the initial step of tissue culture for the recovery of the fast disappearing Rhizophora annamalayana Kathir. Further research is progressing on mass multiplication and field transfer.http://www.plant-journal.uaic.ro/docs/2013/7.pdfmangrovesin vitro propagationgrowth regulators |
| spellingShingle | KATHIRESAN Kandasamy RAVINDER Singh Chinnappan PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD Journal of Plant Development mangroves in vitro propagation growth regulators |
| title | PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD |
| title_full | PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD |
| title_fullStr | PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD |
| title_full_unstemmed | PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD |
| title_short | PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD |
| title_sort | preliminary conservation effort on rhizophora annamalayana kathir the only endemic mangrove to india through in vitro method |
| topic | mangroves in vitro propagation growth regulators |
| url | http://www.plant-journal.uaic.ro/docs/2013/7.pdf |
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