Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress
Callose deposition occurs during plant growth and development, as well as when plants are under biotic and abiotic stress. Callose synthase is a key enzyme for the synthesis of callose. In this study, 27, 28, 16, and 15 callose synthase family members were identified in Gossypium hirsutum, Gossypium...
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PeerJ Inc.
2021-11-01
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| Online Access: | https://peerj.com/articles/12557.pdf |
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| author | Jiajia Feng Yi Chen Xianghui Xiao Yunfang Qu Pengtao Li Quanwei Lu Jinling Huang |
| author_facet | Jiajia Feng Yi Chen Xianghui Xiao Yunfang Qu Pengtao Li Quanwei Lu Jinling Huang |
| author_sort | Jiajia Feng |
| collection | DOAJ |
| description | Callose deposition occurs during plant growth and development, as well as when plants are under biotic and abiotic stress. Callose synthase is a key enzyme for the synthesis of callose. In this study, 27, 28, 16, and 15 callose synthase family members were identified in Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum using the sequence of Arabidopsis callose synthase. The CalSs were divided into five groups by phylogenetic, gene structure, and conservative motif analysis. The conserved motifs and gene structures of CalSs in each group were highly similar. Based on the analysis of cis-acting elements, it is inferred that GhCalSs were regulated by abiotic stress. WGD/Segmental duplication promoted the amplification of the CalS gene in cotton, and purification selection had an important function in the CalS family. The transcriptome data and qRT-PCR under cold, heat, salt, and PEG treatments showed that GhCalSs were involved in abiotic stress. The expression patterns of GhCalSs were different in various tissues. We predicted that GhCalS4, which was highly expressed in fibers, had an important effect on fiber elongation. Hence, these results help us understand the role of GhCalSs in fiber development and stress response. |
| first_indexed | 2024-03-09T04:30:52Z |
| format | Article |
| id | doaj.art-6e26bb1ff9f94232b0038e091ad82e37 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T04:30:52Z |
| publishDate | 2021-11-01 |
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| spelling | doaj.art-6e26bb1ff9f94232b0038e091ad82e372023-12-03T13:35:19ZengPeerJ Inc.PeerJ2167-83592021-11-019e1255710.7717/peerj.12557Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stressJiajia Feng0Yi Chen1Xianghui Xiao2Yunfang Qu3Pengtao Li4Quanwei Lu5Jinling Huang6College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, ChinaSchool of Biotechnology and Food Engineering, Anyang Institute of Technology, Anyang, Henan, ChinaCollege of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, ChinaSchool of Biotechnology and Food Engineering, Anyang Institute of Technology, Anyang, Henan, ChinaCollege of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, ChinaCallose deposition occurs during plant growth and development, as well as when plants are under biotic and abiotic stress. Callose synthase is a key enzyme for the synthesis of callose. In this study, 27, 28, 16, and 15 callose synthase family members were identified in Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum using the sequence of Arabidopsis callose synthase. The CalSs were divided into five groups by phylogenetic, gene structure, and conservative motif analysis. The conserved motifs and gene structures of CalSs in each group were highly similar. Based on the analysis of cis-acting elements, it is inferred that GhCalSs were regulated by abiotic stress. WGD/Segmental duplication promoted the amplification of the CalS gene in cotton, and purification selection had an important function in the CalS family. The transcriptome data and qRT-PCR under cold, heat, salt, and PEG treatments showed that GhCalSs were involved in abiotic stress. The expression patterns of GhCalSs were different in various tissues. We predicted that GhCalS4, which was highly expressed in fibers, had an important effect on fiber elongation. Hence, these results help us understand the role of GhCalSs in fiber development and stress response.https://peerj.com/articles/12557.pdfCallose synthaseSyntenyGene familyGossypiumVarious stresses |
| spellingShingle | Jiajia Feng Yi Chen Xianghui Xiao Yunfang Qu Pengtao Li Quanwei Lu Jinling Huang Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress PeerJ Callose synthase Synteny Gene family Gossypium Various stresses |
| title | Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress |
| title_full | Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress |
| title_fullStr | Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress |
| title_full_unstemmed | Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress |
| title_short | Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress |
| title_sort | genome wide analysis of the cals gene family in cotton reveals their potential roles in fiber development and responses to stress |
| topic | Callose synthase Synteny Gene family Gossypium Various stresses |
| url | https://peerj.com/articles/12557.pdf |
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