Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
Abstract This study aimed to develop a protocol for the cryopreservation of Pseudoplatystoma corruscans semen. For this, mature males were hormonally induced with a single dose of carp pituitary extract (5 mg/kg body weight). Semen was collected and evaluated. Two cryoprotectants were tested to comp...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Ictiologia
2023-02-01
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| Series: | Neotropical Ichthyology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252023000100201&lng=en&tlng=en |
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| author | Johanna Maritza Cano Velarde Natalia Marins Bastos Laícia Carneiro-Leite Laís Pedroso Borges Edson Guilherme Vieira Rosicleire Veríssimo-Silveira Alexandre Ninhaus-Silveira |
| author_facet | Johanna Maritza Cano Velarde Natalia Marins Bastos Laícia Carneiro-Leite Laís Pedroso Borges Edson Guilherme Vieira Rosicleire Veríssimo-Silveira Alexandre Ninhaus-Silveira |
| author_sort | Johanna Maritza Cano Velarde |
| collection | DOAJ |
| description | Abstract This study aimed to develop a protocol for the cryopreservation of Pseudoplatystoma corruscans semen. For this, mature males were hormonally induced with a single dose of carp pituitary extract (5 mg/kg body weight). Semen was collected and evaluated. Two cryoprotectants were tested to compose the diluents: dimethyl acetamide (DMA) and dimethyl sulfoxide (Me2SO), in two concentrations (8% and 10%), + 5.0% glucose + 10% egg yolk. The semen was diluted in a 1: 4 ratio (semen: extender), packed in 0.5 mL straws and frozen in a dry shipper container in liquid nitrogen vapors. After thawing, sperm kinetics, sperm morphology and DNA integrity of cryopreserved sperm were evaluated. Pseudoplatystoma corruscans males produced semen with sperm motility > 80%. After thawing, all treatments provided semen with total sperm motility > 40%, with no significant difference (P < 0.05) between them, as well as between the other sperm kinetic parameters evaluated. The treatments with DMA provided a smaller fragmentation of the DNA of the gametes. Sperm malformations were identified in both fresh and cryopreserved semen, with a slight increase in these malformations being identified in sperm from thawed P. corruscans semen samples. |
| first_indexed | 2024-04-10T09:05:30Z |
| format | Article |
| id | doaj.art-6e2ab5e25db649a7a899e071e205de17 |
| institution | Directory Open Access Journal |
| issn | 1982-0224 |
| language | English |
| last_indexed | 2024-04-10T09:05:30Z |
| publishDate | 2023-02-01 |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj.art-6e2ab5e25db649a7a899e071e205de172023-02-21T07:32:47ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02242023-02-0121110.1590/1982-0224-2022-0071Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semenJohanna Maritza Cano Velardehttps://orcid.org/0000-0002-4366-3512Natalia Marins Bastoshttps://orcid.org/0000-0001-8189-5085Laícia Carneiro-Leitehttps://orcid.org/0000-0002-5128-3739Laís Pedroso Borgeshttps://orcid.org/0000-0002-1792-1530Edson Guilherme Vieirahttps://orcid.org/0000-0003-1911-2750Rosicleire Veríssimo-Silveirahttps://orcid.org/0000-0002-8298-5004Alexandre Ninhaus-Silveirahttps://orcid.org/0000-0003-0738-0862Abstract This study aimed to develop a protocol for the cryopreservation of Pseudoplatystoma corruscans semen. For this, mature males were hormonally induced with a single dose of carp pituitary extract (5 mg/kg body weight). Semen was collected and evaluated. Two cryoprotectants were tested to compose the diluents: dimethyl acetamide (DMA) and dimethyl sulfoxide (Me2SO), in two concentrations (8% and 10%), + 5.0% glucose + 10% egg yolk. The semen was diluted in a 1: 4 ratio (semen: extender), packed in 0.5 mL straws and frozen in a dry shipper container in liquid nitrogen vapors. After thawing, sperm kinetics, sperm morphology and DNA integrity of cryopreserved sperm were evaluated. Pseudoplatystoma corruscans males produced semen with sperm motility > 80%. After thawing, all treatments provided semen with total sperm motility > 40%, with no significant difference (P < 0.05) between them, as well as between the other sperm kinetic parameters evaluated. The treatments with DMA provided a smaller fragmentation of the DNA of the gametes. Sperm malformations were identified in both fresh and cryopreserved semen, with a slight increase in these malformations being identified in sperm from thawed P. corruscans semen samples.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252023000100201&lng=en&tlng=enFreezingSperm qualityPimelodidaeDNA integrityGermplasm |
| spellingShingle | Johanna Maritza Cano Velarde Natalia Marins Bastos Laícia Carneiro-Leite Laís Pedroso Borges Edson Guilherme Vieira Rosicleire Veríssimo-Silveira Alexandre Ninhaus-Silveira Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen Neotropical Ichthyology Freezing Sperm quality Pimelodidae DNA integrity Germplasm |
| title | Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen |
| title_full | Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen |
| title_fullStr | Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen |
| title_full_unstemmed | Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen |
| title_short | Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen |
| title_sort | dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of pseudoplatystoma corruscans semen |
| topic | Freezing Sperm quality Pimelodidae DNA integrity Germplasm |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252023000100201&lng=en&tlng=en |
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