Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey
Ticks play a pivotal role in propagating a diverse spectrum of infectious agents that detrimentally affect the health of both humans and animals. In the present study, a molecular survey was executed of piroplasmids in ticks collected from small ruminants in four districts within Konya province, Tur...
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MDPI AG
2023-09-01
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| author | Zhuowei Ma Onur Ceylan Eloiza May Galon Uday Kumar Mohanta Shengwei Ji Hang Li Thanh Thom Do Rika Umemiya-Shirafuji Shimaa Abd El-Salam El-Sayed Iqra Zafar Mingming Liu Ferda Sevinc Xuenan Xuan |
| author_facet | Zhuowei Ma Onur Ceylan Eloiza May Galon Uday Kumar Mohanta Shengwei Ji Hang Li Thanh Thom Do Rika Umemiya-Shirafuji Shimaa Abd El-Salam El-Sayed Iqra Zafar Mingming Liu Ferda Sevinc Xuenan Xuan |
| author_sort | Zhuowei Ma |
| collection | DOAJ |
| description | Ticks play a pivotal role in propagating a diverse spectrum of infectious agents that detrimentally affect the health of both humans and animals. In the present study, a molecular survey was executed of piroplasmids in ticks collected from small ruminants in four districts within Konya province, Turkey. Microscopic examination identified 1281 adult ticks, which were categorized into 357 pools based on their species, sexes, host animals, and collection site before DNA extraction. The infection rates were calculated by using a maximum likelihood estimate (MLE) with 95% confidence intervals (CI). <i>Hyalomma detritum</i>, <i>H</i>. <i>excavatum</i>, <i>Rhipicephalus bursa</i>, <i>R</i>. <i>sanguineus</i>, and <i>R</i>. <i>turanicus</i> were identified in this study. Among the five tick species identified here, <i>R</i>. <i>turanicus</i> exhibited the highest infestation rate in both goats and sheep. The presence of <i>Babesia ovis</i> and <i>Theileria ovis</i> based on 18S rRNA was confirmed using molecular assay. The overall MLE of infection rates for <i>B</i>. <i>ovis</i> and <i>T</i>. <i>ovis</i> was 2.49% (CI 1.72–3.46) and 1.46% (CI 0.87–2.23), respectively. The MLE of <i>B</i>. <i>ovis</i> and <i>T</i>. <i>ovis</i> infection rates in <i>R</i>. <i>bursa</i> was 10.80% (CI 7.43–14.90) and 0.33% (CI 0.02–1.42), respectively, while that in <i>R</i>. <i>turanicus</i> was 0.12% (CI 0.01–0.51) and 2.08% (CI 1.25–3.22). This study further confirms that <i>R</i>. <i>turanicus</i> and <i>R</i>. <i>sanguineus</i> can act as vectors for <i>B</i>. <i>ovis</i>, thus advancing our comprehension of tick-borne piroplasmids epidemiology and providing valuable insights for the development of effective control strategies for ticks and tick-borne diseases in Turkey. |
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| spelling | doaj.art-6e2ae049d1304f5db6004154f1b28a902023-11-19T12:22:22ZengMDPI AGPathogens2076-08172023-09-01129112310.3390/pathogens12091123Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, TurkeyZhuowei Ma0Onur Ceylan1Eloiza May Galon2Uday Kumar Mohanta3Shengwei Ji4Hang Li5Thanh Thom Do6Rika Umemiya-Shirafuji7Shimaa Abd El-Salam El-Sayed8Iqra Zafar9Mingming Liu10Ferda Sevinc11Xuenan Xuan12National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanDepartment of Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42250 Konya, TurkeyCollege of Veterinary Medicine and Biomedical Sciences, Cavite State University, Indang 4122, PhilippinesNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanSchool of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, ChinaDepartment of Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42250 Konya, TurkeyNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanTicks play a pivotal role in propagating a diverse spectrum of infectious agents that detrimentally affect the health of both humans and animals. In the present study, a molecular survey was executed of piroplasmids in ticks collected from small ruminants in four districts within Konya province, Turkey. Microscopic examination identified 1281 adult ticks, which were categorized into 357 pools based on their species, sexes, host animals, and collection site before DNA extraction. The infection rates were calculated by using a maximum likelihood estimate (MLE) with 95% confidence intervals (CI). <i>Hyalomma detritum</i>, <i>H</i>. <i>excavatum</i>, <i>Rhipicephalus bursa</i>, <i>R</i>. <i>sanguineus</i>, and <i>R</i>. <i>turanicus</i> were identified in this study. Among the five tick species identified here, <i>R</i>. <i>turanicus</i> exhibited the highest infestation rate in both goats and sheep. The presence of <i>Babesia ovis</i> and <i>Theileria ovis</i> based on 18S rRNA was confirmed using molecular assay. The overall MLE of infection rates for <i>B</i>. <i>ovis</i> and <i>T</i>. <i>ovis</i> was 2.49% (CI 1.72–3.46) and 1.46% (CI 0.87–2.23), respectively. The MLE of <i>B</i>. <i>ovis</i> and <i>T</i>. <i>ovis</i> infection rates in <i>R</i>. <i>bursa</i> was 10.80% (CI 7.43–14.90) and 0.33% (CI 0.02–1.42), respectively, while that in <i>R</i>. <i>turanicus</i> was 0.12% (CI 0.01–0.51) and 2.08% (CI 1.25–3.22). This study further confirms that <i>R</i>. <i>turanicus</i> and <i>R</i>. <i>sanguineus</i> can act as vectors for <i>B</i>. <i>ovis</i>, thus advancing our comprehension of tick-borne piroplasmids epidemiology and providing valuable insights for the development of effective control strategies for ticks and tick-borne diseases in Turkey.https://www.mdpi.com/2076-0817/12/9/1123piroplasmidstick speciesmolecular identificationKonya ProvinceTurkey |
| spellingShingle | Zhuowei Ma Onur Ceylan Eloiza May Galon Uday Kumar Mohanta Shengwei Ji Hang Li Thanh Thom Do Rika Umemiya-Shirafuji Shimaa Abd El-Salam El-Sayed Iqra Zafar Mingming Liu Ferda Sevinc Xuenan Xuan Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey Pathogens piroplasmids tick species molecular identification Konya Province Turkey |
| title | Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey |
| title_full | Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey |
| title_fullStr | Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey |
| title_full_unstemmed | Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey |
| title_short | Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey |
| title_sort | molecular identification of piroplasmids in ticks from infested small ruminants in konya province turkey |
| topic | piroplasmids tick species molecular identification Konya Province Turkey |
| url | https://www.mdpi.com/2076-0817/12/9/1123 |
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