Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection
Background: The aim of the present study was to investigate the susceptibility of purified protein derivative (PPD) plus health-care workers to SARS-CoV-2 (COVID-19). For this reason, single-nucleotide polymorphism (SNP) of interferon-gamma (IFN-γ) gene at position +2109 and IFN-γ receptor 1 (R1) at...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=3;spage=318;epage=322;aulast=Varahram |
| _version_ | 1817983452998270976 |
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| author | Mohammad Varahram Saeid Besharati Poopak Farnia Jalaledin Ghanavi Shima Saif Jalal Heshmat Nia Mohammad Reza Madani Parissa Farnia Ali Akbar Velayati |
| author_facet | Mohammad Varahram Saeid Besharati Poopak Farnia Jalaledin Ghanavi Shima Saif Jalal Heshmat Nia Mohammad Reza Madani Parissa Farnia Ali Akbar Velayati |
| author_sort | Mohammad Varahram |
| collection | DOAJ |
| description | Background: The aim of the present study was to investigate the susceptibility of purified protein derivative (PPD) plus health-care workers to SARS-CoV-2 (COVID-19). For this reason, single-nucleotide polymorphism (SNP) of interferon-gamma (IFN-γ) gene at position +2109 and IFN-γ receptor 1 (R1) at position −56 was assessed in PPD plus group before and after COVID-19 infection (2017–2018; 2020–2021). Methods: The selected study cases (n = 100) that were working in tuberculosis (TB) unite (5–10 years) with PPD positivity >15 mm (16–20 mm) were included in this investigation. Sampling was done twice, once before and after the COVID-19 pandemic. Group A contains 50 samples collected from the GenBank TB laboratory that belong to TB staff before the pandemic (2017–2018). The other sample (Group B; 2021) was collected from the same unite during the COVID-19 pandemic. The SNP in the IFN-γ gene (+2109; 670 bp) and IFN-γ R 1 (−56; 366 bp) was performed using a specific primer and the polymerase chain reaction products were digested using restriction enzyme Fau I and Bts I, respectively. Statistical analyses were used to obtain the frequency of alleles among all studied cases. The confidence intervals (CIs) and t-test were calculated using the SPSS and GraphPad Prism software. Results: In overall, the most frequent genotype in Group A was AA (41/50; 82%) and Group B was 76% (38/50) in position + 2109 (odds ratio [OR] = 0.69, 95% CI, 0.26–1.83, and P = 0.46). Although in position −56, the most frequent genotype in Group A was TT (35/50; 70%) which significantly was than Group B TT (15/50; 30%) (OR = 0.184, 95% CI, 0.78–0.43, and P = 0.00). The frequency of allele A was more in both groups at position + 2109 (OR = 0.815, 95% CI, 0.23–2.86, and P = 0.75), whereas the dominate allele at position −56 was T in Group A (OR = 1.37, 95% CI, 0.62–3.02, and P = 0.42). Conclusion: No significant differences were observed in + 2109 in genotype among Group A and B. The main differences were seen in IFN-γ R1 at position (−56) between Group A and B. Hence, the IFN-γ R1 may play important role in COVID-19 infection. However, more study is needed to clear the IFN-γ correlation to COVID-19 infection. |
| first_indexed | 2024-04-13T23:33:26Z |
| format | Article |
| id | doaj.art-6e2b4c6bea9e4fe988dcb7257f9f18aa |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-04-13T23:33:26Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-6e2b4c6bea9e4fe988dcb7257f9f18aa2022-12-22T02:24:50ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2022-01-0111331832210.4103/ijmy.ijmy_133_22Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infectionMohammad VarahramSaeid BesharatiPoopak FarniaJalaledin GhanaviShima SaifJalal Heshmat NiaMohammad Reza MadaniParissa FarniaAli Akbar VelayatiBackground: The aim of the present study was to investigate the susceptibility of purified protein derivative (PPD) plus health-care workers to SARS-CoV-2 (COVID-19). For this reason, single-nucleotide polymorphism (SNP) of interferon-gamma (IFN-γ) gene at position +2109 and IFN-γ receptor 1 (R1) at position −56 was assessed in PPD plus group before and after COVID-19 infection (2017–2018; 2020–2021). Methods: The selected study cases (n = 100) that were working in tuberculosis (TB) unite (5–10 years) with PPD positivity >15 mm (16–20 mm) were included in this investigation. Sampling was done twice, once before and after the COVID-19 pandemic. Group A contains 50 samples collected from the GenBank TB laboratory that belong to TB staff before the pandemic (2017–2018). The other sample (Group B; 2021) was collected from the same unite during the COVID-19 pandemic. The SNP in the IFN-γ gene (+2109; 670 bp) and IFN-γ R 1 (−56; 366 bp) was performed using a specific primer and the polymerase chain reaction products were digested using restriction enzyme Fau I and Bts I, respectively. Statistical analyses were used to obtain the frequency of alleles among all studied cases. The confidence intervals (CIs) and t-test were calculated using the SPSS and GraphPad Prism software. Results: In overall, the most frequent genotype in Group A was AA (41/50; 82%) and Group B was 76% (38/50) in position + 2109 (odds ratio [OR] = 0.69, 95% CI, 0.26–1.83, and P = 0.46). Although in position −56, the most frequent genotype in Group A was TT (35/50; 70%) which significantly was than Group B TT (15/50; 30%) (OR = 0.184, 95% CI, 0.78–0.43, and P = 0.00). The frequency of allele A was more in both groups at position + 2109 (OR = 0.815, 95% CI, 0.23–2.86, and P = 0.75), whereas the dominate allele at position −56 was T in Group A (OR = 1.37, 95% CI, 0.62–3.02, and P = 0.42). Conclusion: No significant differences were observed in + 2109 in genotype among Group A and B. The main differences were seen in IFN-γ R1 at position (−56) between Group A and B. Hence, the IFN-γ R1 may play important role in COVID-19 infection. However, more study is needed to clear the IFN-γ correlation to COVID-19 infection.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=3;spage=318;epage=322;aulast=Varahramcovid-19interferon-gammapolymorphismpurified protein derivativetuberculosis |
| spellingShingle | Mohammad Varahram Saeid Besharati Poopak Farnia Jalaledin Ghanavi Shima Saif Jalal Heshmat Nia Mohammad Reza Madani Parissa Farnia Ali Akbar Velayati Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection International Journal of Mycobacteriology covid-19 interferon-gamma polymorphism purified protein derivative tuberculosis |
| title | Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection |
| title_full | Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection |
| title_fullStr | Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection |
| title_full_unstemmed | Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection |
| title_short | Correlation of single-nucleotide polymorphism at interferon-gamma R1 (at Position − 56) in positive purified protein derivative health workers with COVID-19 infection |
| title_sort | correlation of single nucleotide polymorphism at interferon gamma r1 at position 56 in positive purified protein derivative health workers with covid 19 infection |
| topic | covid-19 interferon-gamma polymorphism purified protein derivative tuberculosis |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2022;volume=11;issue=3;spage=318;epage=322;aulast=Varahram |
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