Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis
Abstract Background/purpose: Sprouty (SPRY) has four isoforms, SPRY1–4, and its deficiency produces haphazard ‘sprouting’ of tracheal tubules. This study investigated SPRY1 protein expression in human oral potentially malignant disorders (OPMDs) and oral squamous cell carcinomas (OSCCs). Materials a...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Dental Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790220301719 |
| _version_ | 1828942071688331264 |
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| author | Yen-Yun Wang Wen-Chen Wang Chiang-Wei Su Ching-Wei Hsu Shyng-Shiou Yuan Yuk-Kwan Chen |
| author_facet | Yen-Yun Wang Wen-Chen Wang Chiang-Wei Su Ching-Wei Hsu Shyng-Shiou Yuan Yuk-Kwan Chen |
| author_sort | Yen-Yun Wang |
| collection | DOAJ |
| description | Abstract Background/purpose: Sprouty (SPRY) has four isoforms, SPRY1–4, and its deficiency produces haphazard ‘sprouting’ of tracheal tubules. This study investigated SPRY1 protein expression in human oral potentially malignant disorders (OPMDs) and oral squamous cell carcinomas (OSCCs). Materials and methods: 90 OSCCs, 10 OPMDs with malignant transformation (MT), 17 OPMDs without MT, and six normal oral mucosa (NOM) tissue samples were subjected to immunohistochemical staining. Three human oral cancer cell lines (OCCLs), an oral precancer cell line (DOK), and a primary culture of normal oral keratinocytes (HOK) were used for western blotting. Results: Significantly increased expression of SPRY1 protein from NOM and OPMD without MT to OSCC was observed. The protein expressions of SPRY1 in OCCLs were significantly enhanced as compared with DOK and HOK. Increased phosphor/total-ERK expression was observed in OCCLs as compared with HOK. A significantly increased SPRY1 protein level was noted in OPMDs with MT as compared with those without MT, in addition to a significant increase in DOK in comparison with HOK. Conclusion: Our results indicated that overexpression of SPRY1 protein is potentially associated with human oral squamous cell carcinogenesis. |
| first_indexed | 2024-12-14T03:48:21Z |
| format | Article |
| id | doaj.art-6e2e1ae3ba00452cb91322092e4e19a9 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-14T03:48:21Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-6e2e1ae3ba00452cb91322092e4e19a92022-12-21T23:18:17ZengElsevierJournal of Dental Sciences1991-79022021-01-011612128Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesisYen-Yun Wang0Wen-Chen Wang1Chiang-Wei Su2Ching-Wei Hsu3Shyng-Shiou Yuan4Yuk-Kwan Chen5School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Oral Pathology & Maxillofacial Radiology, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Oral & Maxillofacial Imaging Center, Kaohsiung Medical University, Kaohsiung, TaiwanDivision of Oral & Maxillofacial Surgery, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDivision of Oral & Maxillofacial Surgery, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDepartment of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology and Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Corresponding author. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan. Fax: +886 7 3210637.School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Oral Pathology & Maxillofacial Radiology, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Oral & Maxillofacial Imaging Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Corresponding author. School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan. Fax: +886 7 3210637.Abstract Background/purpose: Sprouty (SPRY) has four isoforms, SPRY1–4, and its deficiency produces haphazard ‘sprouting’ of tracheal tubules. This study investigated SPRY1 protein expression in human oral potentially malignant disorders (OPMDs) and oral squamous cell carcinomas (OSCCs). Materials and methods: 90 OSCCs, 10 OPMDs with malignant transformation (MT), 17 OPMDs without MT, and six normal oral mucosa (NOM) tissue samples were subjected to immunohistochemical staining. Three human oral cancer cell lines (OCCLs), an oral precancer cell line (DOK), and a primary culture of normal oral keratinocytes (HOK) were used for western blotting. Results: Significantly increased expression of SPRY1 protein from NOM and OPMD without MT to OSCC was observed. The protein expressions of SPRY1 in OCCLs were significantly enhanced as compared with DOK and HOK. Increased phosphor/total-ERK expression was observed in OCCLs as compared with HOK. A significantly increased SPRY1 protein level was noted in OPMDs with MT as compared with those without MT, in addition to a significant increase in DOK in comparison with HOK. Conclusion: Our results indicated that overexpression of SPRY1 protein is potentially associated with human oral squamous cell carcinogenesis.http://www.sciencedirect.com/science/article/pii/S1991790220301719SPRY1Oral squamous cell carcinomaOral potentially malignant disorders |
| spellingShingle | Yen-Yun Wang Wen-Chen Wang Chiang-Wei Su Ching-Wei Hsu Shyng-Shiou Yuan Yuk-Kwan Chen Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis Journal of Dental Sciences SPRY1 Oral squamous cell carcinoma Oral potentially malignant disorders |
| title | Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis |
| title_full | Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis |
| title_fullStr | Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis |
| title_full_unstemmed | Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis |
| title_short | Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis |
| title_sort | overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis |
| topic | SPRY1 Oral squamous cell carcinoma Oral potentially malignant disorders |
| url | http://www.sciencedirect.com/science/article/pii/S1991790220301719 |
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