The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo

Purpose: to evaluate the effects of acute hypoxic hypoxia on eye tissue cells in adult rats and determining the effectiveness of 0.007 % solution of para-aminobenzoic acid (PABA) for apoptotic damage to the tissues of the ocular surface in rats.Materials and methods. In the experiment, 27 male Wista...

Full description

Bibliographic Details
Main Authors: A. A. Ryabtseva, S. I. Akberova, G. Kh. Ali-zade, H. F. Babayev, Yu. V. Markitantova
Format: Article
Language:Russian
Published: Ophthalmology Publishing Group 2021-07-01
Series:Oftalʹmologiâ
Subjects:
Online Access:https://www.ophthalmojournal.com/opht/article/view/1548
_version_ 1797875453919232000
author A. A. Ryabtseva
S. I. Akberova
G. Kh. Ali-zade
H. F. Babayev
Yu. V. Markitantova
author_facet A. A. Ryabtseva
S. I. Akberova
G. Kh. Ali-zade
H. F. Babayev
Yu. V. Markitantova
author_sort A. A. Ryabtseva
collection DOAJ
description Purpose: to evaluate the effects of acute hypoxic hypoxia on eye tissue cells in adult rats and determining the effectiveness of 0.007 % solution of para-aminobenzoic acid (PABA) for apoptotic damage to the tissues of the ocular surface in rats.Materials and methods. In the experiment, 27 male Wistar rats (54 eyes) were used, divided into 5 groups. Group I — intact control, Group II — hypoxic control after 1 hour, Group III — hypoxic control after 3 hours, Group IV — with the injection of the drug 1 hour after hypoxia, Group V — injection of the drug 24 hours before hypoxia. The identification of apoptotic cells in the tissues of the eye was performed by the TUNEL method on frozen sections of the eye stained with Hoechst 33342 nuclear fluorescent dye. The localization and fluorescence intensity of the damaged cells was analyzed under a fluorescence microscope using the Image J. computer program.Results. Under the conditions of simulated acute hypoxic hypoxia, apoptotic lesions of the conjunctiva, corneal epithelium, choroid, and photoreceptor layer of the retina were observed. In group II, the number of damaged cells in the conjunctiva was 67 %, and in group III it was 120 % more compared to group I — the norm (p < 0.05). With the injection of PABA before hypoxia, there were no significant differences compared with the norm (group I) in the state of the affected cells in the conjunctiva. In the group of animals (group III) with the injection of PABA after hypoxia after 1 h, the number of damaged cells does not statistically differ from group II (hypoxic control after 1 h), but significantly lower than in group III (hypoxic control after 3 hours). The same pattern is observed in the corneal epithelium.Conclusion. Para-aminobenzoic acid (0.007 %) has a prophylactic and therapeutic effect, preventing and stabilizing the development of apoptosis of conjunctival cells and anterior corneal epithelium induced in experimental acute hypoxic hypoxia.
first_indexed 2024-04-10T01:46:45Z
format Article
id doaj.art-6e35bf41accd40fbb7e6cc451dfc7c68
institution Directory Open Access Journal
issn 1816-5095
2500-0845
language Russian
last_indexed 2024-04-10T01:46:45Z
publishDate 2021-07-01
publisher Ophthalmology Publishing Group
record_format Article
series Oftalʹmologiâ
spelling doaj.art-6e35bf41accd40fbb7e6cc451dfc7c682023-03-13T09:08:41ZrusOphthalmology Publishing GroupOftalʹmologiâ1816-50952500-08452021-07-0118231732410.18008/1816-5095-2021-2-317-324761The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivoA. A. Ryabtseva0S. I. Akberova1G. Kh. Ali-zade2H. F. Babayev3Yu. V. Markitantova4ГБУЗ МО «Московский областной научно-исследовательский институт им. М.Ф. Владимирского»Медицинский центр «Медквадрат»ГБУЗ МО «Московский областной научно-исследовательский институт им. М.Ф. Владимирского»Институт физиологии им. А.И. Караева НАН АзербайджанаИнститут биологии развития им. Н.К. Кольцова РАНPurpose: to evaluate the effects of acute hypoxic hypoxia on eye tissue cells in adult rats and determining the effectiveness of 0.007 % solution of para-aminobenzoic acid (PABA) for apoptotic damage to the tissues of the ocular surface in rats.Materials and methods. In the experiment, 27 male Wistar rats (54 eyes) were used, divided into 5 groups. Group I — intact control, Group II — hypoxic control after 1 hour, Group III — hypoxic control after 3 hours, Group IV — with the injection of the drug 1 hour after hypoxia, Group V — injection of the drug 24 hours before hypoxia. The identification of apoptotic cells in the tissues of the eye was performed by the TUNEL method on frozen sections of the eye stained with Hoechst 33342 nuclear fluorescent dye. The localization and fluorescence intensity of the damaged cells was analyzed under a fluorescence microscope using the Image J. computer program.Results. Under the conditions of simulated acute hypoxic hypoxia, apoptotic lesions of the conjunctiva, corneal epithelium, choroid, and photoreceptor layer of the retina were observed. In group II, the number of damaged cells in the conjunctiva was 67 %, and in group III it was 120 % more compared to group I — the norm (p < 0.05). With the injection of PABA before hypoxia, there were no significant differences compared with the norm (group I) in the state of the affected cells in the conjunctiva. In the group of animals (group III) with the injection of PABA after hypoxia after 1 h, the number of damaged cells does not statistically differ from group II (hypoxic control after 1 h), but significantly lower than in group III (hypoxic control after 3 hours). The same pattern is observed in the corneal epithelium.Conclusion. Para-aminobenzoic acid (0.007 %) has a prophylactic and therapeutic effect, preventing and stabilizing the development of apoptosis of conjunctival cells and anterior corneal epithelium induced in experimental acute hypoxic hypoxia.https://www.ophthalmojournal.com/opht/article/view/1548глазная поверхностьроговицаконъюнктиваапоптоз клетокпарааминобензойная кислотагипоксия
spellingShingle A. A. Ryabtseva
S. I. Akberova
G. Kh. Ali-zade
H. F. Babayev
Yu. V. Markitantova
The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo
Oftalʹmologiâ
глазная поверхность
роговица
конъюнктива
апоптоз клеток
парааминобензойная кислота
гипоксия
title The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo
title_full The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo
title_fullStr The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo
title_full_unstemmed The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo
title_short The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo
title_sort protective effect of para aminobenzoic acid in hypoxiainduced apoptosis of conjunctiva and cornea cells in vivo
topic глазная поверхность
роговица
конъюнктива
апоптоз клеток
парааминобензойная кислота
гипоксия
url https://www.ophthalmojournal.com/opht/article/view/1548
work_keys_str_mv AT aaryabtseva theprotectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT siakberova theprotectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT gkhalizade theprotectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT hfbabayev theprotectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT yuvmarkitantova theprotectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT aaryabtseva protectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT siakberova protectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT gkhalizade protectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT hfbabayev protectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo
AT yuvmarkitantova protectiveeffectofparaaminobenzoicacidinhypoxiainducedapoptosisofconjunctivaandcorneacellsinvivo