Prognostic significance of snail expression in hilar cholangiocarcinoma
Many patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic sign...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2012-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700008&lng=en&tlng=en |
| _version_ | 1828366426877984768 |
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| author | Dalu Kong Jun Liang Rong Li Shihai Liu Jigang Wang Kejun Zhang Dong Chen |
| author_facet | Dalu Kong Jun Liang Rong Li Shihai Liu Jigang Wang Kejun Zhang Dong Chen |
| author_sort | Dalu Kong |
| collection | DOAJ |
| description | Many patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic significance of snail expression in HC. We examined the protein expression of snail and E-cadherin in HC tissues from 47 patients (22 males and 25 females, mean age 61.2 years) using immunohistochemistry and RT-PCR. Proliferation rate was also evaluated in the same cases by the MIB1 index. High, low and negative snail protein expression was recorded in 18 (38%), 17 (36%), and 12 (26%) cases, respectively, and 40.4% (19/47) cases showed reduced E-cadherin protein expression in HC samples. No significant correlation was found between snail and E-cadherin protein expression levels (P = 0.056). No significant correlation was found between snail protein expression levels and gender, age, tumor grade, vascular or perineural invasion, nodal metastasis and invasion, or proliferative index. Cancer samples with positive snail protein expression were associated with poor survival compared with the negative expresser groups. Kaplan-Meier curves comparing different snail protein expression levels to survival showed highly significant separation (P < 0.0001, log-rank test). With multivariate analysis, only snail protein expression among all parameters was found to influence survival (P = 0.0003). We suggest that snail expression levels can predict poor survival regardless of pathological features and tumor proliferation. Immunohistochemical detection of snail protein expression levels in routine sections may provide the first biological prognostic marker. |
| first_indexed | 2024-04-14T05:44:56Z |
| format | Article |
| id | doaj.art-6e3931b909784ddea6015427d58ecd26 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-14T05:44:56Z |
| publishDate | 2012-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-6e3931b909784ddea6015427d58ecd262022-12-22T02:09:19ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2012-07-01457617624S0100-879X2012000700008Prognostic significance of snail expression in hilar cholangiocarcinomaDalu Kong0Jun Liang1Rong Li2Shihai Liu3Jigang Wang4Kejun Zhang5Dong Chen6Tianjin Medical University Cancer Institute and HospitalQingdao UniversityTianjin Medical University Cancer Institute and HospitalQingdao UniversityQingdao UniversityQingdao UniversityQingdao UniversityMany patients with hilar cholangiocarcinoma (HC) have a poor prognosis. Snail, a transcription factor and E-cadherin repressor, is a novel prognostic factor in many cancers. The aim of this study was to evaluate the relationship between snail and E-cadherin protein expression and the prognostic significance of snail expression in HC. We examined the protein expression of snail and E-cadherin in HC tissues from 47 patients (22 males and 25 females, mean age 61.2 years) using immunohistochemistry and RT-PCR. Proliferation rate was also evaluated in the same cases by the MIB1 index. High, low and negative snail protein expression was recorded in 18 (38%), 17 (36%), and 12 (26%) cases, respectively, and 40.4% (19/47) cases showed reduced E-cadherin protein expression in HC samples. No significant correlation was found between snail and E-cadherin protein expression levels (P = 0.056). No significant correlation was found between snail protein expression levels and gender, age, tumor grade, vascular or perineural invasion, nodal metastasis and invasion, or proliferative index. Cancer samples with positive snail protein expression were associated with poor survival compared with the negative expresser groups. Kaplan-Meier curves comparing different snail protein expression levels to survival showed highly significant separation (P < 0.0001, log-rank test). With multivariate analysis, only snail protein expression among all parameters was found to influence survival (P = 0.0003). We suggest that snail expression levels can predict poor survival regardless of pathological features and tumor proliferation. Immunohistochemical detection of snail protein expression levels in routine sections may provide the first biological prognostic marker.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700008&lng=en&tlng=enHilar cholangiocarcinomaPrognostic markerSnailE-cadherinImmunohistochemistry |
| spellingShingle | Dalu Kong Jun Liang Rong Li Shihai Liu Jigang Wang Kejun Zhang Dong Chen Prognostic significance of snail expression in hilar cholangiocarcinoma Brazilian Journal of Medical and Biological Research Hilar cholangiocarcinoma Prognostic marker Snail E-cadherin Immunohistochemistry |
| title | Prognostic significance of snail expression in hilar cholangiocarcinoma |
| title_full | Prognostic significance of snail expression in hilar cholangiocarcinoma |
| title_fullStr | Prognostic significance of snail expression in hilar cholangiocarcinoma |
| title_full_unstemmed | Prognostic significance of snail expression in hilar cholangiocarcinoma |
| title_short | Prognostic significance of snail expression in hilar cholangiocarcinoma |
| title_sort | prognostic significance of snail expression in hilar cholangiocarcinoma |
| topic | Hilar cholangiocarcinoma Prognostic marker Snail E-cadherin Immunohistochemistry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700008&lng=en&tlng=en |
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