Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity
Introduction: Various kidney diseases reportedly show different urinary extracellular vesicle (EV) RNA profiles. Although obesity is one of the main causes of chronic kidney disease, the expression pattern of urinary EV RNA in obesity is uncertain. Our aim was to sequence the small RNA profiles of u...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2022-01-01
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| Series: | Obesity Facts |
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| Online Access: | https://www.karger.com/Article/FullText/521730 |
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| author | Dughyun Choi Sewon Kim Jeyoung Woo Haekyung Lee Hyoungnae Kim Jin Seok Jeon Hyunjin Noh Dong Cheol Han Sang Hyun Kim Ho Chan Cho Jong-Soo Choi Yun-Ui Bae Soon Hyo Kwon |
| author_facet | Dughyun Choi Sewon Kim Jeyoung Woo Haekyung Lee Hyoungnae Kim Jin Seok Jeon Hyunjin Noh Dong Cheol Han Sang Hyun Kim Ho Chan Cho Jong-Soo Choi Yun-Ui Bae Soon Hyo Kwon |
| author_sort | Dughyun Choi |
| collection | DOAJ |
| description | Introduction: Various kidney diseases reportedly show different urinary extracellular vesicle (EV) RNA profiles. Although obesity is one of the main causes of chronic kidney disease, the expression pattern of urinary EV RNA in obesity is uncertain. Our aim was to sequence the small RNA profiles of urinary EVs in obese patients before and after weight reduction and compare them to those of healthy volunteers (HVs). Methods: We recruited age-sex-matched obese patients and HVs. The small RNA profiles of urinary EVs were analyzed using RNA sequencing. To evaluate the effect of weight reduction, small RNA profiles of urinary EVs 6 months after bariatric surgery were also analyzed. Results: The proportion of urinary EVs transfer RNA and microRNA of obese patients differed from that of HVs. Obese patients showed differential expression of 1,343 small RNAs in urinary EVs compared to HVs (fold change ≥2 and p value <0.05). Among those, 61 small RNAs were upregulated in obese patients and downregulated after weight reduction, whereas 167 small RNAs were downregulated in obese patients and upregulated after weight reduction. RNA sequencing revealed the correlation between the specific urinary EV small RNAs and clinical parameters including body weight, low-density lipoprotein cholesterol, triglyceride, high-density lipoprotein cholesterol, serum glucose, estimated glomerular filtration rate, and albuminuria. Conclusion: Obese patients showed distinct urinary EV small RNA profiles compared to HVs. Weight reduction altered urinary EV small-RNA profiles in obese patients. |
| first_indexed | 2024-12-13T01:04:33Z |
| format | Article |
| id | doaj.art-6e3f236dd2884e668ba5259106096235 |
| institution | Directory Open Access Journal |
| issn | 1662-4025 1662-4033 |
| language | English |
| last_indexed | 2024-12-13T01:04:33Z |
| publishDate | 2022-01-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Obesity Facts |
| spelling | doaj.art-6e3f236dd2884e668ba52591060962352022-12-22T00:04:35ZengKarger PublishersObesity Facts1662-40251662-40332022-01-0111010.1159/000521730521730Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in ObesityDughyun Choi0https://orcid.org/0000-0002-9175-6641Sewon Kim1Jeyoung Woo2Haekyung Lee3Hyoungnae Kim4Jin Seok Jeon5Hyunjin Noh6Dong Cheol Han7Sang Hyun Kim8Ho Chan Cho9Jong-Soo Choi10Yun-Ui Bae11Soon Hyo Kwon12https://orcid.org/0000-0002-4114-4196Division of Endocrinology and Metabolism, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Republic of KoreaMacrogen Inc., Seoul, Republic of KoreaMacrogen Inc., Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaDepartment of Surgery, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaDepartment of Clinical Endocrinology, Keimyung University School of Medicine, Daegu, Republic of KoreaResearch Institute, Biosolyx Co. Ltd., Daegu, Republic of KoreaDepartment of Clinical Endocrinology, Keimyung University School of Medicine, Daegu, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, Republic of KoreaIntroduction: Various kidney diseases reportedly show different urinary extracellular vesicle (EV) RNA profiles. Although obesity is one of the main causes of chronic kidney disease, the expression pattern of urinary EV RNA in obesity is uncertain. Our aim was to sequence the small RNA profiles of urinary EVs in obese patients before and after weight reduction and compare them to those of healthy volunteers (HVs). Methods: We recruited age-sex-matched obese patients and HVs. The small RNA profiles of urinary EVs were analyzed using RNA sequencing. To evaluate the effect of weight reduction, small RNA profiles of urinary EVs 6 months after bariatric surgery were also analyzed. Results: The proportion of urinary EVs transfer RNA and microRNA of obese patients differed from that of HVs. Obese patients showed differential expression of 1,343 small RNAs in urinary EVs compared to HVs (fold change ≥2 and p value <0.05). Among those, 61 small RNAs were upregulated in obese patients and downregulated after weight reduction, whereas 167 small RNAs were downregulated in obese patients and upregulated after weight reduction. RNA sequencing revealed the correlation between the specific urinary EV small RNAs and clinical parameters including body weight, low-density lipoprotein cholesterol, triglyceride, high-density lipoprotein cholesterol, serum glucose, estimated glomerular filtration rate, and albuminuria. Conclusion: Obese patients showed distinct urinary EV small RNA profiles compared to HVs. Weight reduction altered urinary EV small-RNA profiles in obese patients.https://www.karger.com/Article/FullText/521730extracellular vesiclernasmall untranslatedobesity |
| spellingShingle | Dughyun Choi Sewon Kim Jeyoung Woo Haekyung Lee Hyoungnae Kim Jin Seok Jeon Hyunjin Noh Dong Cheol Han Sang Hyun Kim Ho Chan Cho Jong-Soo Choi Yun-Ui Bae Soon Hyo Kwon Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity Obesity Facts extracellular vesicle rna small untranslated obesity |
| title | Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity |
| title_full | Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity |
| title_fullStr | Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity |
| title_full_unstemmed | Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity |
| title_short | Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity |
| title_sort | weight change alters the small rna profile of urinary extracellular vesicles in obesity |
| topic | extracellular vesicle rna small untranslated obesity |
| url | https://www.karger.com/Article/FullText/521730 |
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