Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity
This study was undertaken to screen for some biomarkers of toxicity in the liver of Nile tilapia fish during subacute Diazinon toxicity (0.28 mgL-1 for 25 days) by using Targeted metabolomics analyses and quantitatively measure 17 amino acids, and also to monitor antioxidant status of liver (glutat...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-08-01
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| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1379 |
| _version_ | 1797657050583400448 |
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| author | Eman A. Kamal Nevien K.M. Abdelkhalek Azza E.A. Hassan Mohamed El-Adl |
| author_facet | Eman A. Kamal Nevien K.M. Abdelkhalek Azza E.A. Hassan Mohamed El-Adl |
| author_sort | Eman A. Kamal |
| collection | DOAJ |
| description |
This study was undertaken to screen for some biomarkers of toxicity in the liver of Nile tilapia fish during subacute Diazinon toxicity (0.28 mgL-1 for 25 days) by using Targeted metabolomics analyses and quantitatively measure 17 amino acids, and also to monitor antioxidant status of liver (glutathione, peroxidase, catalase, superoxide dismutase and malondialdehyde). There were significant increases in branched chain amino acids valine, leucine and isoleucine (p>0.01, p>0.05and p>0.01) respectively. There was a significant increase in phenylalanine (an aromatic amino acid) P>0.05, a significant increase in lipid peroxidation (malondialdehyde P>0.001), and significant decreases in the activity of antioxidant enzymes (SOD, CAT, GSH-px) with p values (P>0.01, P>0.01, and P> 0.001) respectively. Histopathological examination showed diffuse hepatocellular necrosis with multifocal granuloma and massive hepatocellular vacuolation with congested sinusoids. It can be concluded that subacute toxicity of DZN in Nile tilapia is involved in proliferation and growth of tumor cells and negatively affects the antioxidant status of the liver.
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| first_indexed | 2024-03-11T17:39:48Z |
| format | Article |
| id | doaj.art-6e3f8a9d644e4ee28a0a186808bd27a5 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:39:48Z |
| publishDate | 2023-08-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-6e3f8a9d644e4ee28a0a186808bd27a52023-10-18T14:10:32ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-08-01136Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon ToxicityEman A. Kamal0Nevien K.M. Abdelkhalek1Azza E.A. Hassan2Mohamed El-Adl3Animal Health Research Institute, Mansoura, Egypt.Department of Internal medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Egypt.Animal Health Research Institute, Mansoura, Egypt.Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Egypt. This study was undertaken to screen for some biomarkers of toxicity in the liver of Nile tilapia fish during subacute Diazinon toxicity (0.28 mgL-1 for 25 days) by using Targeted metabolomics analyses and quantitatively measure 17 amino acids, and also to monitor antioxidant status of liver (glutathione, peroxidase, catalase, superoxide dismutase and malondialdehyde). There were significant increases in branched chain amino acids valine, leucine and isoleucine (p>0.01, p>0.05and p>0.01) respectively. There was a significant increase in phenylalanine (an aromatic amino acid) P>0.05, a significant increase in lipid peroxidation (malondialdehyde P>0.001), and significant decreases in the activity of antioxidant enzymes (SOD, CAT, GSH-px) with p values (P>0.01, P>0.01, and P> 0.001) respectively. Histopathological examination showed diffuse hepatocellular necrosis with multifocal granuloma and massive hepatocellular vacuolation with congested sinusoids. It can be concluded that subacute toxicity of DZN in Nile tilapia is involved in proliferation and growth of tumor cells and negatively affects the antioxidant status of the liver. https://advetresearch.com/index.php/AVR/article/view/1379 AntioxidantBiomarkersDiazinon (DZN)MetabolomicsNile tilapiasubacute toxicity |
| spellingShingle | Eman A. Kamal Nevien K.M. Abdelkhalek Azza E.A. Hassan Mohamed El-Adl Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity Journal of Advanced Veterinary Research Antioxidant Biomarkers Diazinon (DZN) Metabolomics Nile tilapia subacute toxicity |
| title | Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity |
| title_full | Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity |
| title_fullStr | Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity |
| title_full_unstemmed | Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity |
| title_short | Metabolomic Analyses, Toxicity Biomarkers and Histopathological Changes in the Liver of Nile Tilapia Exposed to Diazinon Toxicity |
| title_sort | metabolomic analyses toxicity biomarkers and histopathological changes in the liver of nile tilapia exposed to diazinon toxicity |
| topic | Antioxidant Biomarkers Diazinon (DZN) Metabolomics Nile tilapia subacute toxicity |
| url | https://advetresearch.com/index.php/AVR/article/view/1379 |
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