The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O
Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escher...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.633706/full |
| _version_ | 1831636954365558784 |
|---|---|
| author | Yan Xiao Yan Xiao Suling Zhang He Yan Xiaolin Geng Yanwei Wang Xin Xu Mengyue Wang Haohao Zhang Baicheng Huang Wenqiang Pang Ming Yang Kegong Tian Kegong Tian |
| author_facet | Yan Xiao Yan Xiao Suling Zhang He Yan Xiaolin Geng Yanwei Wang Xin Xu Mengyue Wang Haohao Zhang Baicheng Huang Wenqiang Pang Ming Yang Kegong Tian Kegong Tian |
| author_sort | Yan Xiao |
| collection | DOAJ |
| description | Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine. |
| first_indexed | 2024-12-19T06:42:22Z |
| format | Article |
| id | doaj.art-6e40e60ec64347239e1c6a555e997a28 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-19T06:42:22Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-6e40e60ec64347239e1c6a555e997a282022-12-21T20:32:01ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-02-01810.3389/fvets.2021.633706633706The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype OYan Xiao0Yan Xiao1Suling Zhang2He Yan3Xiaolin Geng4Yanwei Wang5Xin Xu6Mengyue Wang7Haohao Zhang8Baicheng Huang9Wenqiang Pang10Ming Yang11Kegong Tian12Kegong Tian13College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaNational Centre for Foreign Animal Disease, Winnipeg, MB, CanadaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaNational Research Center for Veterinary Medicine, Luoyang, ChinaFoot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.https://www.frontiersin.org/articles/10.3389/fvets.2021.633706/fullfoot-and-mouth disease virus serotype Ovirus-like particlesneutralizing epitopeshumoral immunitycellular immunity |
| spellingShingle | Yan Xiao Yan Xiao Suling Zhang He Yan Xiaolin Geng Yanwei Wang Xin Xu Mengyue Wang Haohao Zhang Baicheng Huang Wenqiang Pang Ming Yang Kegong Tian Kegong Tian The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O Frontiers in Veterinary Science foot-and-mouth disease virus serotype O virus-like particles neutralizing epitopes humoral immunity cellular immunity |
| title | The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O |
| title_full | The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O |
| title_fullStr | The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O |
| title_full_unstemmed | The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O |
| title_short | The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O |
| title_sort | high immunity induced by the virus like particles of foot and mouth disease virus serotype o |
| topic | foot-and-mouth disease virus serotype O virus-like particles neutralizing epitopes humoral immunity cellular immunity |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.633706/full |
| work_keys_str_mv | AT yanxiao thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT yanxiao thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT sulingzhang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT heyan thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT xiaolingeng thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT yanweiwang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT xinxu thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT mengyuewang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT haohaozhang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT baichenghuang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT wenqiangpang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT mingyang thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT kegongtian thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT kegongtian thehighimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT yanxiao highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT yanxiao highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT sulingzhang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT heyan highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT xiaolingeng highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT yanweiwang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT xinxu highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT mengyuewang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT haohaozhang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT baichenghuang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT wenqiangpang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT mingyang highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT kegongtian highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo AT kegongtian highimmunityinducedbytheviruslikeparticlesoffootandmouthdiseasevirusserotypeo |