UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
As a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/11/2659 |
| _version_ | 1827676587880873984 |
|---|---|
| author | Wei Xiang Zhining Xia Li Xu |
| author_facet | Wei Xiang Zhining Xia Li Xu |
| author_sort | Wei Xiang |
| collection | DOAJ |
| description | As a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed by UPLC-MS/MS, and 42 components, including alkaloids, flavonoids, and coumarins, were obtained. Then, the antioxidant activities, hypoglycemic effect, Alzheimer’s disease inhibition, and cardiovascular protection of MBE were also evaluated in vitro. The IC<sub>50</sub> values for the scavenging DPPH and ABTS radicals were, respectively, 31.23 ± 0.57 μg/mL and 8.88 ± 0.36 μg/mL (IC<sub>50</sub> values of positive Vc were, respectively, 4.41 ± 0.19 μg/mL and 8.79 ± 0.41 μg/mL). The IC<sub>50</sub> value for inhibiting α-glucosidase was 1.90 ± 0.05 μg/mL (IC<sub>50</sub> value of positive acarbose was 0.03 μg/mL). The IC<sub>50</sub> values for inhibiting acetylcholinesterase and butyrylcholinesterase were, respectively, 179.47 ± 0.38 μg/mL and 101.82 ± 3.37 μg/mL (IC<sub>50</sub> values of positive berberine were, respectively, 1.27 ± 0.03 μg/mL and 57.41 ± 0.21 μg/mL). MBE (10 μg/mL and 40 μg/mL) significantly increased the survival rate of oxidized low-density lipoprotein- (ox-LDL) induced human umbilical vein endothelial cells (HUVECs) and significantly decreased the intracellular reactive oxygen species. These results suggest that the extracts of Jialing 20 mulberry branches could be used as a functional food additive. |
| first_indexed | 2024-03-10T05:30:29Z |
| format | Article |
| id | doaj.art-6e40f01a61084fc2bd85a7d9a67350c6 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T05:30:29Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-6e40f01a61084fc2bd85a7d9a67350c62023-11-22T23:20:14ZengMDPI AGFoods2304-81582021-11-011011265910.3390/foods10112659UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch ExtractWei Xiang0Zhining Xia1Li Xu2School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, ChinaSchool of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, ChinaState Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaAs a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed by UPLC-MS/MS, and 42 components, including alkaloids, flavonoids, and coumarins, were obtained. Then, the antioxidant activities, hypoglycemic effect, Alzheimer’s disease inhibition, and cardiovascular protection of MBE were also evaluated in vitro. The IC<sub>50</sub> values for the scavenging DPPH and ABTS radicals were, respectively, 31.23 ± 0.57 μg/mL and 8.88 ± 0.36 μg/mL (IC<sub>50</sub> values of positive Vc were, respectively, 4.41 ± 0.19 μg/mL and 8.79 ± 0.41 μg/mL). The IC<sub>50</sub> value for inhibiting α-glucosidase was 1.90 ± 0.05 μg/mL (IC<sub>50</sub> value of positive acarbose was 0.03 μg/mL). The IC<sub>50</sub> values for inhibiting acetylcholinesterase and butyrylcholinesterase were, respectively, 179.47 ± 0.38 μg/mL and 101.82 ± 3.37 μg/mL (IC<sub>50</sub> values of positive berberine were, respectively, 1.27 ± 0.03 μg/mL and 57.41 ± 0.21 μg/mL). MBE (10 μg/mL and 40 μg/mL) significantly increased the survival rate of oxidized low-density lipoprotein- (ox-LDL) induced human umbilical vein endothelial cells (HUVECs) and significantly decreased the intracellular reactive oxygen species. These results suggest that the extracts of Jialing 20 mulberry branches could be used as a functional food additive.https://www.mdpi.com/2304-8158/10/11/2659mulberry branchUPLC-MSantioxidantα-glucosidasecholinesterasecardiovascular |
| spellingShingle | Wei Xiang Zhining Xia Li Xu UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract Foods mulberry branch UPLC-MS antioxidant α-glucosidase cholinesterase cardiovascular |
| title | UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract |
| title_full | UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract |
| title_fullStr | UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract |
| title_full_unstemmed | UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract |
| title_short | UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract |
| title_sort | uplc ms ms profiling antioxidant α glucosidase inhibitory cholinesterase inhibitory and cardiovascular protection potentials of jialing 20 i morus multicaulis i perr mulberry branch extract |
| topic | mulberry branch UPLC-MS antioxidant α-glucosidase cholinesterase cardiovascular |
| url | https://www.mdpi.com/2304-8158/10/11/2659 |
| work_keys_str_mv | AT weixiang uplcmsmsprofilingantioxidantaglucosidaseinhibitorycholinesteraseinhibitoryandcardiovascularprotectionpotentialsofjialing20imorusmulticaulisiperrmulberrybranchextract AT zhiningxia uplcmsmsprofilingantioxidantaglucosidaseinhibitorycholinesteraseinhibitoryandcardiovascularprotectionpotentialsofjialing20imorusmulticaulisiperrmulberrybranchextract AT lixu uplcmsmsprofilingantioxidantaglucosidaseinhibitorycholinesteraseinhibitoryandcardiovascularprotectionpotentialsofjialing20imorusmulticaulisiperrmulberrybranchextract |