Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration
Tumour survival and growth are reliant on angiogenesis, the formation of new blood vessels, to facilitate nutrient and waste exchange and, importantly, provide a route for metastasis from a primary to a secondary site. Whilst current models can ensure the transport and exchange of nutrients and wast...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1321197/full |
| _version_ | 1797362469524471808 |
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| author | Caitlin E. Jackson Caitlin E. Jackson Iona Doyle Hamood Khan Samuel F. Williams Betül Aldemir Dikici Edgar Barajas Ledesma Helen E. Bryant William R. English Nicola H. Green Nicola H. Green Frederik Claeyssens Frederik Claeyssens |
| author_facet | Caitlin E. Jackson Caitlin E. Jackson Iona Doyle Hamood Khan Samuel F. Williams Betül Aldemir Dikici Edgar Barajas Ledesma Helen E. Bryant William R. English Nicola H. Green Nicola H. Green Frederik Claeyssens Frederik Claeyssens |
| author_sort | Caitlin E. Jackson |
| collection | DOAJ |
| description | Tumour survival and growth are reliant on angiogenesis, the formation of new blood vessels, to facilitate nutrient and waste exchange and, importantly, provide a route for metastasis from a primary to a secondary site. Whilst current models can ensure the transport and exchange of nutrients and waste via diffusion over distances greater than 200 μm, many lack sufficient vasculature capable of recapitulating the tumour microenvironment and, thus, metastasis. In this study, we utilise gelatin-containing polymerised high internal phase emulsion (polyHIPE) templated polycaprolactone-methacrylate (PCL-M) scaffolds to fabricate a composite material to support the 3D culture of MDA-MB-231 breast cancer cells and vascular ingrowth. Firstly, we investigated the effect of gelatin within the scaffolds on the mechanical and chemical properties using compression testing and FTIR spectroscopy, respectively. Initial in vitro assessment of cell metabolic activity and vascular endothelial growth factor expression demonstrated that gelatin-containing PCL-M polyHIPEs are capable of supporting 3D breast cancer cell growth. We then utilised the chick chorioallantoic membrane (CAM) assay to assess the angiogenic potential of cell-seeded gelatin-containing PCL-M polyHIPEs, and vascular ingrowth within cell-seeded, surfactant and gelatin-containing scaffolds was investigated via histological staining. Overall, our study proposes a promising composite material to fabricate a substrate to support the 3D culture of cancer cells and vascular ingrowth. |
| first_indexed | 2024-03-08T16:08:24Z |
| format | Article |
| id | doaj.art-6e434095490c4230bbdd3443b1e0c64b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T16:08:24Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-6e434095490c4230bbdd3443b1e0c64b2024-01-08T04:33:21ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-01-011110.3389/fbioe.2023.13211971321197Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltrationCaitlin E. Jackson0Caitlin E. Jackson1Iona Doyle2Hamood Khan3Samuel F. Williams4Betül Aldemir Dikici5Edgar Barajas Ledesma6Helen E. Bryant7William R. English8Nicola H. Green9Nicola H. Green10Frederik Claeyssens11Frederik Claeyssens12The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United KingdomInsigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United KingdomThe Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United KingdomThe Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United KingdomDepartment of Infection, Immunity and Cardiovascular Disease, Royal Hallamshire Hospital, The University of Sheffield, Sheffield, United KingdomDepartment of Bioengineering, Izmir Institute of Technology, Urla, TürkiyeDepartment of Chemistry, The University of Sheffield, Sheffield, United KingdomSchool of Medicine and Population Health, University of Sheffield, Sheffield, United KingdomNorwich Medical School, University of East Anglia, Norwich, United KingdomThe Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United KingdomInsigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United KingdomThe Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United KingdomInsigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United KingdomTumour survival and growth are reliant on angiogenesis, the formation of new blood vessels, to facilitate nutrient and waste exchange and, importantly, provide a route for metastasis from a primary to a secondary site. Whilst current models can ensure the transport and exchange of nutrients and waste via diffusion over distances greater than 200 μm, many lack sufficient vasculature capable of recapitulating the tumour microenvironment and, thus, metastasis. In this study, we utilise gelatin-containing polymerised high internal phase emulsion (polyHIPE) templated polycaprolactone-methacrylate (PCL-M) scaffolds to fabricate a composite material to support the 3D culture of MDA-MB-231 breast cancer cells and vascular ingrowth. Firstly, we investigated the effect of gelatin within the scaffolds on the mechanical and chemical properties using compression testing and FTIR spectroscopy, respectively. Initial in vitro assessment of cell metabolic activity and vascular endothelial growth factor expression demonstrated that gelatin-containing PCL-M polyHIPEs are capable of supporting 3D breast cancer cell growth. We then utilised the chick chorioallantoic membrane (CAM) assay to assess the angiogenic potential of cell-seeded gelatin-containing PCL-M polyHIPEs, and vascular ingrowth within cell-seeded, surfactant and gelatin-containing scaffolds was investigated via histological staining. Overall, our study proposes a promising composite material to fabricate a substrate to support the 3D culture of cancer cells and vascular ingrowth.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1321197/fullgelatinpolyHIPECAM assayPCL (polycaprolactone)vascularisationangiogenesis |
| spellingShingle | Caitlin E. Jackson Caitlin E. Jackson Iona Doyle Hamood Khan Samuel F. Williams Betül Aldemir Dikici Edgar Barajas Ledesma Helen E. Bryant William R. English Nicola H. Green Nicola H. Green Frederik Claeyssens Frederik Claeyssens Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration Frontiers in Bioengineering and Biotechnology gelatin polyHIPE CAM assay PCL (polycaprolactone) vascularisation angiogenesis |
| title | Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration |
| title_full | Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration |
| title_fullStr | Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration |
| title_full_unstemmed | Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration |
| title_short | Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration |
| title_sort | gelatin containing porous polycaprolactone polyhipes as substrates for 3d breast cancer cell culture and vascular infiltration |
| topic | gelatin polyHIPE CAM assay PCL (polycaprolactone) vascularisation angiogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1321197/full |
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