Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure.
INTRODUCTION:The glomerular filtration rate (GFR) is considered an especially important tool for the measurement of renal function. Inulin clearance (InCl) is the classic reference method for this purpose, although it is associated with a number of disadvantages; thus, other markers have been propos...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4395274?pdf=render |
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| author | Michelle T Passos Sonia K Nishida Niels O S Câmara Maria Heloisa Shimizu Gianna Mastroianni-Kirsztajn |
| author_facet | Michelle T Passos Sonia K Nishida Niels O S Câmara Maria Heloisa Shimizu Gianna Mastroianni-Kirsztajn |
| author_sort | Michelle T Passos |
| collection | DOAJ |
| description | INTRODUCTION:The glomerular filtration rate (GFR) is considered an especially important tool for the measurement of renal function. Inulin clearance (InCl) is the classic reference method for this purpose, although it is associated with a number of disadvantages; thus, other markers have been proposed, including iohexol. Determination of iohexol clearance (IoCl) has been established for clinical use; however, its application as a GFR marker in experimental rat models has not been reported. OBJECTIVES:This study aims to standardize a methodology for the measurement of iohexol clearance and to evaluate its applicability as a marker of GFR in rats with induced toxic acute renal failure (ARF), using InCl as the gold standard. MATERIALS AND METHODS:Twenty-six Wistar male rats (200-300 g) were divided into the following two groups: a control group (n=7) and an ARF group (n=19). ARF was induced by the subcutaneous administration of cisplatin (5 mg/kg); IoCl and InCl were determined simultaneously, and plasma creatinine (pCreat) dosage was measured colorimetrically. RESULTS:The pCreat, InCl and IoCl levels were consistent with the expected values for the renal function ranges of the evaluated animals, and the IoCl and InCl levels were significantly correlated (r=0.792). An inverse moderate linear correlation between the IoCl and pCreat measurements (r=-0.587) and between the InCl and pCreat measurements (r=-0.722) were observed. CONCLUSION:These results confirm a correlation between IoCl and the gold standard of GFR, InCl measurement. IoCl offers a relevant advantage over InCl because determination of the former allows the animal to live after the procedure. |
| first_indexed | 2024-12-20T07:13:44Z |
| format | Article |
| id | doaj.art-6e445936aeb74b5092ad0910959aad95 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:13:44Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6e445936aeb74b5092ad0910959aad952022-12-21T19:48:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012375310.1371/journal.pone.0123753Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure.Michelle T PassosSonia K NishidaNiels O S CâmaraMaria Heloisa ShimizuGianna Mastroianni-KirsztajnINTRODUCTION:The glomerular filtration rate (GFR) is considered an especially important tool for the measurement of renal function. Inulin clearance (InCl) is the classic reference method for this purpose, although it is associated with a number of disadvantages; thus, other markers have been proposed, including iohexol. Determination of iohexol clearance (IoCl) has been established for clinical use; however, its application as a GFR marker in experimental rat models has not been reported. OBJECTIVES:This study aims to standardize a methodology for the measurement of iohexol clearance and to evaluate its applicability as a marker of GFR in rats with induced toxic acute renal failure (ARF), using InCl as the gold standard. MATERIALS AND METHODS:Twenty-six Wistar male rats (200-300 g) were divided into the following two groups: a control group (n=7) and an ARF group (n=19). ARF was induced by the subcutaneous administration of cisplatin (5 mg/kg); IoCl and InCl were determined simultaneously, and plasma creatinine (pCreat) dosage was measured colorimetrically. RESULTS:The pCreat, InCl and IoCl levels were consistent with the expected values for the renal function ranges of the evaluated animals, and the IoCl and InCl levels were significantly correlated (r=0.792). An inverse moderate linear correlation between the IoCl and pCreat measurements (r=-0.587) and between the InCl and pCreat measurements (r=-0.722) were observed. CONCLUSION:These results confirm a correlation between IoCl and the gold standard of GFR, InCl measurement. IoCl offers a relevant advantage over InCl because determination of the former allows the animal to live after the procedure.http://europepmc.org/articles/PMC4395274?pdf=render |
| spellingShingle | Michelle T Passos Sonia K Nishida Niels O S Câmara Maria Heloisa Shimizu Gianna Mastroianni-Kirsztajn Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure. PLoS ONE |
| title | Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure. |
| title_full | Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure. |
| title_fullStr | Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure. |
| title_full_unstemmed | Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure. |
| title_short | Iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure. |
| title_sort | iohexol clearance for determination of glomerular filtration rate in rats induced to acute renal failure |
| url | http://europepmc.org/articles/PMC4395274?pdf=render |
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