COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS
Objective: This study aims to investigate the characteristics of androgen receptors (AR), estrogen receptor 1 (ER1) and estrogen receptor 2 (ER2) expression in dartos tissue of patients with congenital hypospadias, compared to normal penis. Material & Methods: We harvested 63 dartos tissue cons...
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| Format: | Article |
| Language: | English |
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IKATAN AHLI UROLOGI INDONESIA
2023-01-01
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| Series: | Jurnal Urologi Indonesia |
| Subjects: | |
| Online Access: | https://juri.urologi.or.id/juri/article/view/773 |
| _version_ | 1797965553544986624 |
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| author | Aria Danurdoro Tanaya Ghinorawa Prahara Yuri Didik Setyo Hariyanto Arry Rodjani Irfan Wahyudi Yonas Immanuel Hutasoit Andre Yudha Alfanius Hutahaean |
| author_facet | Aria Danurdoro Tanaya Ghinorawa Prahara Yuri Didik Setyo Hariyanto Arry Rodjani Irfan Wahyudi Yonas Immanuel Hutasoit Andre Yudha Alfanius Hutahaean |
| author_sort | Aria Danurdoro |
| collection | DOAJ |
| description |
Objective: This study aims to investigate the characteristics of androgen receptors (AR), estrogen receptor 1 (ER1) and estrogen receptor 2 (ER2) expression in dartos tissue of patients with congenital hypospadias, compared to normal penis. Material & Methods: We harvested 63 dartos tissue consisting of 53 congenital hypospadias that underwent urethroplasty (20 distal and 33 proximal) and 10 normal penis that underwent circumcision as controls from September 2017 to September 2018. The expressions of AR, ER1, and ER2 were measured using Quantitative Real-Time PCR (qPCR). All data were analyzed by Prism 7, and one-way ANOVA tests were used to compare gene expressions between the groups. Results: The mean age was 68.99 (± 45.5) and 65.6 (± 25.8) months in boys with and without hypospadias, respectively. The expression of mRNA AR was decreased in proximal (6.26 ± 2.30) and distal hypospadias (6.43 ± 2.22) compared to controls (9.69 ± 1.10), which were statistically significant (p=0.0001 and p<0.0001, respectively). We found a statistically significant difference of ER1 expression compared to controls (p=0.0064). The expression of ER2 was significantly increased in distal (21.03 ± 5.00) and proximal hypospadias (25.21 ± 8.06) groups compared to controls (11.80 ± 2.49) (p<0.0001). There was no statistically significant mean difference in mRNA ER1 expression (p=0.65). Conclusion: The repressed AR and elevated ER mRNA as shown in our study may suggest that defects in those receptors’ interaction and/or balance may contribute to hypospadias and penile curvature condition. Further studies are needed to evaluate any gene-related problems in hypospadias.
Keywords: Hypospadias, androgen receptor, estrogen receptor, dartos tissue.
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| first_indexed | 2024-04-11T02:00:42Z |
| format | Article |
| id | doaj.art-6e4d0a42a3864e24ba61e9c176684ea5 |
| institution | Directory Open Access Journal |
| issn | 0853-442X 2355-1402 |
| language | English |
| last_indexed | 2024-04-11T02:00:42Z |
| publishDate | 2023-01-01 |
| publisher | IKATAN AHLI UROLOGI INDONESIA |
| record_format | Article |
| series | Jurnal Urologi Indonesia |
| spelling | doaj.art-6e4d0a42a3864e24ba61e9c176684ea52023-01-03T04:06:44ZengIKATAN AHLI UROLOGI INDONESIAJurnal Urologi Indonesia0853-442X2355-14022023-01-0130110.32421/juri.v30i1.773COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIASAria Danurdoro0Tanaya Ghinorawa1Prahara Yuri2Didik Setyo Hariyanto3Arry Rodjani4Irfan Wahyudi5Yonas Immanuel Hutasoit6Andre Yudha Alfanius Hutahaean7Division of Urology, Department of Surgery, Faculty of Medicine/Gadjah Mada University, Sardjito General Hospital, YogyakartaDivision of Urology, Department of Surgery, Faculty of Medicine/Gadjah Mada University, Sardjito General Hospital, YogyakartaDivision of Urology, Department of Surgery, Faculty of Medicine/Gadjah Mada University, Sardjito General Hospital, YogyakartaDepartment of Anatomical Pathology, Faculty of Medicine/Gadjah Mada University, Sardjito General Hospital, YogyakartaDepartment of Urology, Faculty of Medicine/Universitas Indonesia, Cipto Mangunkusumo General Hospital, JakartaDepartment of Urology, Faculty of Medicine/Universitas Indonesia, Cipto Mangunkusumo General Hospital, JakartaDivision of Urology, Department of Surgery/Faculty of Medicine, Fatmawati Hospital, JakartaDivision of Urology, Department of Surgery, Harapan Kita Mother and Children Hospital, Jakarta Objective: This study aims to investigate the characteristics of androgen receptors (AR), estrogen receptor 1 (ER1) and estrogen receptor 2 (ER2) expression in dartos tissue of patients with congenital hypospadias, compared to normal penis. Material & Methods: We harvested 63 dartos tissue consisting of 53 congenital hypospadias that underwent urethroplasty (20 distal and 33 proximal) and 10 normal penis that underwent circumcision as controls from September 2017 to September 2018. The expressions of AR, ER1, and ER2 were measured using Quantitative Real-Time PCR (qPCR). All data were analyzed by Prism 7, and one-way ANOVA tests were used to compare gene expressions between the groups. Results: The mean age was 68.99 (± 45.5) and 65.6 (± 25.8) months in boys with and without hypospadias, respectively. The expression of mRNA AR was decreased in proximal (6.26 ± 2.30) and distal hypospadias (6.43 ± 2.22) compared to controls (9.69 ± 1.10), which were statistically significant (p=0.0001 and p<0.0001, respectively). We found a statistically significant difference of ER1 expression compared to controls (p=0.0064). The expression of ER2 was significantly increased in distal (21.03 ± 5.00) and proximal hypospadias (25.21 ± 8.06) groups compared to controls (11.80 ± 2.49) (p<0.0001). There was no statistically significant mean difference in mRNA ER1 expression (p=0.65). Conclusion: The repressed AR and elevated ER mRNA as shown in our study may suggest that defects in those receptors’ interaction and/or balance may contribute to hypospadias and penile curvature condition. Further studies are needed to evaluate any gene-related problems in hypospadias. Keywords: Hypospadias, androgen receptor, estrogen receptor, dartos tissue. https://juri.urologi.or.id/juri/article/view/773Hypospadiasandrogen receptorestrogen receptordartos tissue |
| spellingShingle | Aria Danurdoro Tanaya Ghinorawa Prahara Yuri Didik Setyo Hariyanto Arry Rodjani Irfan Wahyudi Yonas Immanuel Hutasoit Andre Yudha Alfanius Hutahaean COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS Jurnal Urologi Indonesia Hypospadias androgen receptor estrogen receptor dartos tissue |
| title | COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS |
| title_full | COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS |
| title_fullStr | COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS |
| title_full_unstemmed | COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS |
| title_short | COMPARISON OF ANDROGEN AND ESTROGEN RECEPTORS’ EXPRESSION IN DARTOS TISSUE OF BOYS WITH AND WITHOUT HYPOSPADIAS |
| title_sort | comparison of androgen and estrogen receptors expression in dartos tissue of boys with and without hypospadias |
| topic | Hypospadias androgen receptor estrogen receptor dartos tissue |
| url | https://juri.urologi.or.id/juri/article/view/773 |
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