Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation
In the majority of Mycoplasma synoviae (MS) strains their VLHA haemagglutinins cleave into N-terminal and C-terminal parts. In MS strain ULB 925 (clone KF9) it was found that monoclonal antibodies (mAbs) 3E10, 5G6 and 4G2 recognise the C-terminal parts of its haemagglutinin, designated pMSA, as inte...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2001-06-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/15585 |
| _version_ | 1826532233899409408 |
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| author | Dušan BENČINA Mojca NARAT |
| author_facet | Dušan BENČINA Mojca NARAT |
| author_sort | Dušan BENČINA |
| collection | DOAJ |
| description | In the majority of Mycoplasma synoviae (MS) strains their VLHA haemagglutinins cleave into N-terminal and C-terminal parts. In MS strain ULB 925 (clone KF9) it was found that monoclonal antibodies (mAbs) 3E10, 5G6 and 4G2 recognise the C-terminal parts of its haemagglutinin, designated pMSA, as integral membrane proteins of 51 and 53 kDa. In the same MS clone mAb 125 recognized the N- terminal parts, designated pMSB, as integral membrane proteins of 47 and 49 kDa. However, in MS strain K1723 mAbs 3E10, 5G6 and 125 reacted with an uncleaved VLHA haemagglutinin of about 90 kDa. In MS strain ULB 925 clones their haemagglutinin- positive (HA+) and haemadsorption- positive (HAD+) phenotypes synthesised pMSA defined by mAbs. About 1 % of the populations which ceased to produce pMSA and produced truncated pMSB1 forms (of 27 to 30 kDa) lost the HA+ and HAD+ phenotype. The switching of the synthesis of pMSA proteins was heritable and usually reversible. In strain ULB 925 (clone KF9) certain populations which ceased to produce pMSA defined by mAb 3E10 started to produce pMSB antigenic variant, recognised by another mAb. In MS strains, besides antigenic- variation of pMSA and pMSB, their size- variants were also observed and they were recognised by antibodies of chickens infected with MS. |
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| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2025-03-14T01:47:59Z |
| publishDate | 2001-06-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
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| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-6e4e4dd1ec64486f8ec41788ca952faf2025-03-12T12:22:21ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412001-06-01781152910.14720/aas.2001.78.1.1558521978Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variationDušan BENČINA0Mojca NARAT1Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, SloveniaIn the majority of Mycoplasma synoviae (MS) strains their VLHA haemagglutinins cleave into N-terminal and C-terminal parts. In MS strain ULB 925 (clone KF9) it was found that monoclonal antibodies (mAbs) 3E10, 5G6 and 4G2 recognise the C-terminal parts of its haemagglutinin, designated pMSA, as integral membrane proteins of 51 and 53 kDa. In the same MS clone mAb 125 recognized the N- terminal parts, designated pMSB, as integral membrane proteins of 47 and 49 kDa. However, in MS strain K1723 mAbs 3E10, 5G6 and 125 reacted with an uncleaved VLHA haemagglutinin of about 90 kDa. In MS strain ULB 925 clones their haemagglutinin- positive (HA+) and haemadsorption- positive (HAD+) phenotypes synthesised pMSA defined by mAbs. About 1 % of the populations which ceased to produce pMSA and produced truncated pMSB1 forms (of 27 to 30 kDa) lost the HA+ and HAD+ phenotype. The switching of the synthesis of pMSA proteins was heritable and usually reversible. In strain ULB 925 (clone KF9) certain populations which ceased to produce pMSA defined by mAb 3E10 started to produce pMSB antigenic variant, recognised by another mAb. In MS strains, besides antigenic- variation of pMSA and pMSB, their size- variants were also observed and they were recognised by antibodies of chickens infected with MS.https://journals.uni-lj.si/aas/article/view/15585microbiologymycoplasma synoviaeimmunologyhaemagglutinationmembrane proteinsprotein variation |
| spellingShingle | Dušan BENČINA Mojca NARAT Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation Acta Agriculturae Slovenica microbiology mycoplasma synoviae immunology haemagglutination membrane proteins protein variation |
| title | Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation |
| title_full | Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation |
| title_fullStr | Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation |
| title_full_unstemmed | Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation |
| title_short | Mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase- size- and antigenic-variation |
| title_sort | mycoplasma synoviae haemagglutination involves immunodominant surface membrane proteins undergoing phase size and antigenic variation |
| topic | microbiology mycoplasma synoviae immunology haemagglutination membrane proteins protein variation |
| url | https://journals.uni-lj.si/aas/article/view/15585 |
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