Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study
In the present study, we assessed the ability of MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) to identify microbial strains subjected to high hydrostatic pressure (HHP) as a stress factor. Protein changes induced by HHP can affect the identification of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/813 |
| _version_ | 1797566736979984384 |
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| author | Joanna Bucka-Kolendo Barbara Sokołowska Stanisław Winiarczyk |
| author_facet | Joanna Bucka-Kolendo Barbara Sokołowska Stanisław Winiarczyk |
| author_sort | Joanna Bucka-Kolendo |
| collection | DOAJ |
| description | In the present study, we assessed the ability of MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) to identify microbial strains subjected to high hydrostatic pressure (HHP) as a stress factor. Protein changes induced by HHP can affect the identification of microorganisms when the identification technique is based on the protein profile. We evaluated two methods, namely MALDI-TOF MS and 16S rDNA sequencing, as a valuable tool to identify <i>Lactobacillus</i> species isolated from spoiled food, juices and beers. The data obtained from the protein mass fingerprint analysis of some of the lactobacilli strains showed differences in unpressured and pressured mass spectrum profiles (MSPs), which influenced the results of the identification. Four out of 13 strains (30%) showed different MSP results for unpressured and pressured samples and these results did not overlap with the 16S rDNA identification results. The 16S rDNA sequencing method revealed that five unpressured strains (38%) and four pressured strains (40%) were identified correctly by MALDI-TOF MS. Both methods showed compatible results in 38% of unpressured strains and in 30% of pressured strains. Stress factors, cultivation methods or the natural environment from which the bacteria were derived can affect their protein profile and thus change the mass spectrum. It is necessary to expand the database with a wide range of mass spectra dedicated to a high-throughput study of the microorganisms derived from different environments. |
| first_indexed | 2024-03-10T19:31:34Z |
| format | Article |
| id | doaj.art-6e4e741e677e4f9fad2c744bd5d3489b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:31:34Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6e4e741e677e4f9fad2c744bd5d3489b2023-11-20T02:06:06ZengMDPI AGMicroorganisms2076-26072020-05-018681310.3390/microorganisms8060813Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary StudyJoanna Bucka-Kolendo0Barbara Sokołowska1Stanisław Winiarczyk2Department of Microbiology, Institute of Agriculture and Food Biotechnology, 02-532 Warsaw, PolandDepartment of Microbiology, Institute of Agriculture and Food Biotechnology, 02-532 Warsaw, PolandFaculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, PolandIn the present study, we assessed the ability of MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) to identify microbial strains subjected to high hydrostatic pressure (HHP) as a stress factor. Protein changes induced by HHP can affect the identification of microorganisms when the identification technique is based on the protein profile. We evaluated two methods, namely MALDI-TOF MS and 16S rDNA sequencing, as a valuable tool to identify <i>Lactobacillus</i> species isolated from spoiled food, juices and beers. The data obtained from the protein mass fingerprint analysis of some of the lactobacilli strains showed differences in unpressured and pressured mass spectrum profiles (MSPs), which influenced the results of the identification. Four out of 13 strains (30%) showed different MSP results for unpressured and pressured samples and these results did not overlap with the 16S rDNA identification results. The 16S rDNA sequencing method revealed that five unpressured strains (38%) and four pressured strains (40%) were identified correctly by MALDI-TOF MS. Both methods showed compatible results in 38% of unpressured strains and in 30% of pressured strains. Stress factors, cultivation methods or the natural environment from which the bacteria were derived can affect their protein profile and thus change the mass spectrum. It is necessary to expand the database with a wide range of mass spectra dedicated to a high-throughput study of the microorganisms derived from different environments.https://www.mdpi.com/2076-2607/8/6/813<i>Lactobacillus</i>lactic acid bacteriahigh hydrostatic pressureMALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
| spellingShingle | Joanna Bucka-Kolendo Barbara Sokołowska Stanisław Winiarczyk Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study Microorganisms <i>Lactobacillus</i> lactic acid bacteria high hydrostatic pressure MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
| title | Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study |
| title_full | Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study |
| title_fullStr | Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study |
| title_full_unstemmed | Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study |
| title_short | Influence of High Hydrostatic Pressure on the Identification of <i>Lactobacillus</i> by MALDI-TOF MS- Preliminary Study |
| title_sort | influence of high hydrostatic pressure on the identification of i lactobacillus i by maldi tof ms preliminary study |
| topic | <i>Lactobacillus</i> lactic acid bacteria high hydrostatic pressure MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
| url | https://www.mdpi.com/2076-2607/8/6/813 |
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