Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study
Helicobacter pylori infection changes gastric microbiota profiles. However, it is not clear whether H. pylori eradication can restore the healthy gastric microbiota. Moreover, there has been no study regarding the changes in gastric microbiota with aging. The objective of this study was to investiga...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.621879/full |
| _version_ | 1818414619666939904 |
|---|---|
| author | Cheol Min Shin Nayoung Kim Nayoung Kim Ji Hyun Park Dong Ho Lee Dong Ho Lee |
| author_facet | Cheol Min Shin Nayoung Kim Nayoung Kim Ji Hyun Park Dong Ho Lee Dong Ho Lee |
| author_sort | Cheol Min Shin |
| collection | DOAJ |
| description | Helicobacter pylori infection changes gastric microbiota profiles. However, it is not clear whether H. pylori eradication can restore the healthy gastric microbiota. Moreover, there has been no study regarding the changes in gastric microbiota with aging. The objective of this study was to investigate the changes in gastric corpus microbiota with age and following H. pylori eradication. Changes in corpus mucosa-associated microbiota were evaluated in 43 individuals with endoscopic follow-up > 1 year, including 8 H. pylori-uninfected and 15 H. pylori-infected subjects with no atrophy/metaplasia by histology and pepsinogen I/II ratio > 4.0; 17 H. pylori-infected subjects with atrophy/metaplasia and pepsinogen I/II ratio < 2.5; and 3 subjects with atrophy/metaplasia, no evidence of active H. pylori infection, negative for anti-H. pylori immunoglobulin G (IgG) antibody testing, and no previous history of H. pylori eradication. Successful H. pylori eradication was achieved in 21 patients. The gastric microbiota was characterized using an Illumina MiSeq platform targeting 16S ribosomal DNA (rDNA). The mean follow-up duration was 57.4 months (range, 12–145 months), and median follow-up visit was 1 (range, 1–3). Relative abundance of Lactobacillales and Streptococcus was increased with atrophy/metaplasia. In H. pylori-uninfected subjects (n = 8), an increase in Proteobacteria (Enhydrobacter, Comamonadaceae, Sphingobium); a decrease in Firmicutes (Streptococcus, Veillonella), Fusobacteria (Fusobacterium), Nocardioidaceae, Rothia, and Prevotella; and a decrease in microbial diversity were observed during the follow-up (p trend < 0.05). In 10 of 21 subjects (47.6%), H. pylori eradication induced restoration of microbial diversity; however, a predominance of Acinetobacter with a decrease in microbial diversity occurred in 11 subjects (52.3%). The presence of atrophy/metaplasia at baseline and higher neutrophil infiltration in the corpus were associated with the restoration of gastric microbiota after successful eradication, whereas a higher relative abundance of Acinetobacter at baseline was associated with the predominance of Acinetobacter after H. pylori eradication (p < 0.05). To conclude, in H. pylori-uninfected stomach, relative abundance of Proteobacteria increases, relative abundance of Firmicutes and Fusobacteria decreases, and microbial diversity decreases with aging. H. pylori eradication does not always restore gastric microbiota; in some individuals, gastric colonization by Acinetobacter species occurs after anti-Helicobacter treatment. |
| first_indexed | 2024-12-14T11:21:59Z |
| format | Article |
| id | doaj.art-6e4f91c37ab54af7ad937f98afc1e5a2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T11:21:59Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6e4f91c37ab54af7ad937f98afc1e5a22022-12-21T23:03:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-02-011110.3389/fmicb.2020.621879621879Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up StudyCheol Min Shin0Nayoung Kim1Nayoung Kim2Ji Hyun Park3Dong Ho Lee4Dong Ho Lee5Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam-si, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam-si, South KoreaDepartment of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Seoul National University Bundang Hospital, Seongnam-si, South KoreaDepartment of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, South KoreaHelicobacter pylori infection changes gastric microbiota profiles. However, it is not clear whether H. pylori eradication can restore the healthy gastric microbiota. Moreover, there has been no study regarding the changes in gastric microbiota with aging. The objective of this study was to investigate the changes in gastric corpus microbiota with age and following H. pylori eradication. Changes in corpus mucosa-associated microbiota were evaluated in 43 individuals with endoscopic follow-up > 1 year, including 8 H. pylori-uninfected and 15 H. pylori-infected subjects with no atrophy/metaplasia by histology and pepsinogen I/II ratio > 4.0; 17 H. pylori-infected subjects with atrophy/metaplasia and pepsinogen I/II ratio < 2.5; and 3 subjects with atrophy/metaplasia, no evidence of active H. pylori infection, negative for anti-H. pylori immunoglobulin G (IgG) antibody testing, and no previous history of H. pylori eradication. Successful H. pylori eradication was achieved in 21 patients. The gastric microbiota was characterized using an Illumina MiSeq platform targeting 16S ribosomal DNA (rDNA). The mean follow-up duration was 57.4 months (range, 12–145 months), and median follow-up visit was 1 (range, 1–3). Relative abundance of Lactobacillales and Streptococcus was increased with atrophy/metaplasia. In H. pylori-uninfected subjects (n = 8), an increase in Proteobacteria (Enhydrobacter, Comamonadaceae, Sphingobium); a decrease in Firmicutes (Streptococcus, Veillonella), Fusobacteria (Fusobacterium), Nocardioidaceae, Rothia, and Prevotella; and a decrease in microbial diversity were observed during the follow-up (p trend < 0.05). In 10 of 21 subjects (47.6%), H. pylori eradication induced restoration of microbial diversity; however, a predominance of Acinetobacter with a decrease in microbial diversity occurred in 11 subjects (52.3%). The presence of atrophy/metaplasia at baseline and higher neutrophil infiltration in the corpus were associated with the restoration of gastric microbiota after successful eradication, whereas a higher relative abundance of Acinetobacter at baseline was associated with the predominance of Acinetobacter after H. pylori eradication (p < 0.05). To conclude, in H. pylori-uninfected stomach, relative abundance of Proteobacteria increases, relative abundance of Firmicutes and Fusobacteria decreases, and microbial diversity decreases with aging. H. pylori eradication does not always restore gastric microbiota; in some individuals, gastric colonization by Acinetobacter species occurs after anti-Helicobacter treatment.https://www.frontiersin.org/articles/10.3389/fmicb.2020.621879/fullHelicobacter pylorigastric microbiotaeradicationintestinal metaplasiaatrophic gastritis |
| spellingShingle | Cheol Min Shin Nayoung Kim Nayoung Kim Ji Hyun Park Dong Ho Lee Dong Ho Lee Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study Frontiers in Microbiology Helicobacter pylori gastric microbiota eradication intestinal metaplasia atrophic gastritis |
| title | Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study |
| title_full | Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study |
| title_fullStr | Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study |
| title_full_unstemmed | Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study |
| title_short | Changes in Gastric Corpus Microbiota With Age and After Helicobacter pylori Eradication: A Long-Term Follow-Up Study |
| title_sort | changes in gastric corpus microbiota with age and after helicobacter pylori eradication a long term follow up study |
| topic | Helicobacter pylori gastric microbiota eradication intestinal metaplasia atrophic gastritis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.621879/full |
| work_keys_str_mv | AT cheolminshin changesingastriccorpusmicrobiotawithageandafterhelicobacterpylorieradicationalongtermfollowupstudy AT nayoungkim changesingastriccorpusmicrobiotawithageandafterhelicobacterpylorieradicationalongtermfollowupstudy AT nayoungkim changesingastriccorpusmicrobiotawithageandafterhelicobacterpylorieradicationalongtermfollowupstudy AT jihyunpark changesingastriccorpusmicrobiotawithageandafterhelicobacterpylorieradicationalongtermfollowupstudy AT dongholee changesingastriccorpusmicrobiotawithageandafterhelicobacterpylorieradicationalongtermfollowupstudy AT dongholee changesingastriccorpusmicrobiotawithageandafterhelicobacterpylorieradicationalongtermfollowupstudy |