Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets
Lactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese. The present study was designed to evaluate the effects of Lactobacillus delbrueckii on serum biochemical parameters, intestinal morphology, and performance by supplementing at a dietary le...
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| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.692389/full |
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| author | Xiao-Long Wang Zhu-Ying Liu Ying-Hui Li Ling-Yuan Yang Jie Yin Jian-Hua He De-Xing Hou Ya-Li Liu Xing-Guo Huang |
| author_facet | Xiao-Long Wang Zhu-Ying Liu Ying-Hui Li Ling-Yuan Yang Jie Yin Jian-Hua He De-Xing Hou Ya-Li Liu Xing-Guo Huang |
| author_sort | Xiao-Long Wang |
| collection | DOAJ |
| description | Lactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese. The present study was designed to evaluate the effects of Lactobacillus delbrueckii on serum biochemical parameters, intestinal morphology, and performance by supplementing at a dietary level of 0.1% in diets for weaned piglets. Eighty healthy weaned piglets (initial body weight: 7.56 ± 0.2 kg) were randomly divided into two feeding groups with four replicates in each group (n = 10 animals per replicate); piglets were fed with basal diet (CON) or basal diet containing 0.1% Lactobacillus delbrueckii (LAC). The results showed that dietary supplementation of Lactobacillus delbrueckii improved growth performance and increased serum HDL and insulin levels in piglets on the 28th day of the experimental time (p < 0.05). The gut microbe analysis revealed that Lactobacillus delbrueckii significantly decreased the relative abundance of the phyla Bacteroidetes, but increased the relative abundance of the phyla Firmicutes. The Lactobacillus delbrueckii also significantly increased the relative abundance of Bifidobacterium and Lactobacillus at the genus level of the bacterial community in the ileum, but decreased the relative abundance of unclassified Clostridiales. Moreover, Lactobacillus delbrueckii improved mucosal morphology by obtaining higher intestinal villus height (p < 0.05), significantly increasing the concentrations of butyrate, isobutyric acid, and isovaleric acid in colonic chyme of piglets, but decreasing the intestinal pH at the duodenum and ileum on the 28th day of the experimental time. In conclusion, dietary supplementation of Lactobacillus delbrueckii in the diet of weaned piglets can improve intestinal morphology and modulate the microbiota community to promote growth performance. |
| first_indexed | 2024-12-21T20:55:05Z |
| format | Article |
| id | doaj.art-6e5798af5c324ddb8e4c1adb80ab09ff |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-21T20:55:05Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-6e5798af5c324ddb8e4c1adb80ab09ff2022-12-21T18:50:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-08-01810.3389/fvets.2021.692389692389Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned PigletsXiao-Long Wang0Zhu-Ying Liu1Ying-Hui Li2Ling-Yuan Yang3Jie Yin4Jian-Hua He5De-Xing Hou6Ya-Li Liu7Xing-Guo Huang8College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaThe United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, JapanHunan Pufeike Biotechnology Company, Changsha, ChinaCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaLactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese. The present study was designed to evaluate the effects of Lactobacillus delbrueckii on serum biochemical parameters, intestinal morphology, and performance by supplementing at a dietary level of 0.1% in diets for weaned piglets. Eighty healthy weaned piglets (initial body weight: 7.56 ± 0.2 kg) were randomly divided into two feeding groups with four replicates in each group (n = 10 animals per replicate); piglets were fed with basal diet (CON) or basal diet containing 0.1% Lactobacillus delbrueckii (LAC). The results showed that dietary supplementation of Lactobacillus delbrueckii improved growth performance and increased serum HDL and insulin levels in piglets on the 28th day of the experimental time (p < 0.05). The gut microbe analysis revealed that Lactobacillus delbrueckii significantly decreased the relative abundance of the phyla Bacteroidetes, but increased the relative abundance of the phyla Firmicutes. The Lactobacillus delbrueckii also significantly increased the relative abundance of Bifidobacterium and Lactobacillus at the genus level of the bacterial community in the ileum, but decreased the relative abundance of unclassified Clostridiales. Moreover, Lactobacillus delbrueckii improved mucosal morphology by obtaining higher intestinal villus height (p < 0.05), significantly increasing the concentrations of butyrate, isobutyric acid, and isovaleric acid in colonic chyme of piglets, but decreasing the intestinal pH at the duodenum and ileum on the 28th day of the experimental time. In conclusion, dietary supplementation of Lactobacillus delbrueckii in the diet of weaned piglets can improve intestinal morphology and modulate the microbiota community to promote growth performance.https://www.frontiersin.org/articles/10.3389/fvets.2021.692389/fullLactobacillus delbrueckiimucosal morphologygut microbesshort chain fatty acidsweaned piglets |
| spellingShingle | Xiao-Long Wang Zhu-Ying Liu Ying-Hui Li Ling-Yuan Yang Jie Yin Jian-Hua He De-Xing Hou Ya-Li Liu Xing-Guo Huang Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets Frontiers in Veterinary Science Lactobacillus delbrueckii mucosal morphology gut microbes short chain fatty acids weaned piglets |
| title | Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets |
| title_full | Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets |
| title_fullStr | Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets |
| title_full_unstemmed | Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets |
| title_short | Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets |
| title_sort | effects of dietary supplementation of lactobacillus delbrueckii on gut microbiome and intestinal morphology in weaned piglets |
| topic | Lactobacillus delbrueckii mucosal morphology gut microbes short chain fatty acids weaned piglets |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.692389/full |
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