Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus
Abstract Mosquito-borne diseases represent a growing health challenge over time. Numerous potential phytochemicals are target-specific, biodegradable, and eco-friendly. The larvicidal activity of essential oils, a jasmine blend consisting of Jasmine oil and Azores jasmine (AJ) (Jasminum sambac and J...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-56802-y |
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| author | Ibrahim Taha Radwan Hanem F. Khater Shaimaa H. Mohammed Abdelwahab Khalil Mohamed A. Farghali Mohammed G. Mahmoud Abdelfattah Selim Eman A. Manaa Noha Bagato Mohamed M. Baz |
| author_facet | Ibrahim Taha Radwan Hanem F. Khater Shaimaa H. Mohammed Abdelwahab Khalil Mohamed A. Farghali Mohammed G. Mahmoud Abdelfattah Selim Eman A. Manaa Noha Bagato Mohamed M. Baz |
| author_sort | Ibrahim Taha Radwan |
| collection | DOAJ |
| description | Abstract Mosquito-borne diseases represent a growing health challenge over time. Numerous potential phytochemicals are target-specific, biodegradable, and eco-friendly. The larvicidal activity of essential oils, a jasmine blend consisting of Jasmine oil and Azores jasmine (AJ) (Jasminum sambac and Jasminum azoricum) and peppermint (PP) Mentha arvensis and their nanoformulations against 2nd and 4th instar larvae of Culex pipiens, was evaluated after subjecting to different concentrations (62.5, 125, 250, 500, 1000, and 2000 ppm). Two forms of phase-different nanodelivery systems of layered double hydroxide LDH and oil/water nanoemulsions were formulated. The synthesized nanoemulsions showed particle sizes of 199 and 333 nm for AJ-NE and PP-NE, with a polydispersity index of 0.249 and 0.198, respectively. Chemical and physiochemical analysis of TEM, SEM, XRD, zeta potential, drug loading capacity, and drug release measurements were done to confirm the synthesis and loading efficiencies of essential oils' active ingredients. At high concentrations of AJ and PP nanoemulsions (2000 ppm), O/W nanoemulsions showed higher larval mortality than both LDH conjugates and crude oils. The mortality rate reached 100% for 2nd and 4th instar larvae. The relative toxicities revealed that PP nanoemulsion (MA-NE) was the most effective larvicide, followed by AJ nanoemulsion (AJ-NE). There was a significant increase in defensive enzymes, phenoloxidase, and α and β-esterase enzymes in the treated groups. After treatment of L4 with AJ, AJ-NE, PP, and PP-NE, the levels of phenoloxidase were 545.67, 731.00, 700.00, and 799.67 u/mg, respectively, compared with control 669.67 u/mg. The activity levels of α-esterase were 9.71, 10.32, 8.91, and 10.55 mg α-naphthol/min/mg protein, respectively. It could be concluded that the AJ-NE and PP-NE nanoformulations have promising larvicidal activity and could act as safe and effective alternatives to chemical insecticides. |
| first_indexed | 2024-04-24T19:58:10Z |
| format | Article |
| id | doaj.art-6e59bf3d0d864c7dab2ab198c836ad0d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T19:58:10Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6e59bf3d0d864c7dab2ab198c836ad0d2024-03-24T12:15:42ZengNature PortfolioScientific Reports2045-23222024-03-0114112210.1038/s41598-024-56802-ySynthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens LinnaeusIbrahim Taha Radwan0Hanem F. Khater1Shaimaa H. Mohammed2Abdelwahab Khalil3Mohamed A. Farghali4Mohammed G. Mahmoud5Abdelfattah Selim6Eman A. Manaa7Noha Bagato8Mohamed M. Baz9Supplementary General Sciences Department, Faculty of Oral and Dental Medicine, Future University in EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, Benha UniversityZoology and Entomology Department, Faculty of Science, Al-Azhar, University (Girls Branch)Entomology Division, Zoology Department, Faculty of Science, Beni-Suef UniversityNanotechnology and Advanced Materials Central Lab (NAMCL), Regional Center for Food & Feed (RCFF), Agricultural Research Center (ARC)Plant Protection Department, Faculty of Agriculture, Zagazig UniversityDepartment of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha UniversityAnimal and Poultry Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha UniversityEgyptian Petroleum Research Institute (EPRI)Departments of Entomology, Faculty of Science, Benha UniversityAbstract Mosquito-borne diseases represent a growing health challenge over time. Numerous potential phytochemicals are target-specific, biodegradable, and eco-friendly. The larvicidal activity of essential oils, a jasmine blend consisting of Jasmine oil and Azores jasmine (AJ) (Jasminum sambac and Jasminum azoricum) and peppermint (PP) Mentha arvensis and their nanoformulations against 2nd and 4th instar larvae of Culex pipiens, was evaluated after subjecting to different concentrations (62.5, 125, 250, 500, 1000, and 2000 ppm). Two forms of phase-different nanodelivery systems of layered double hydroxide LDH and oil/water nanoemulsions were formulated. The synthesized nanoemulsions showed particle sizes of 199 and 333 nm for AJ-NE and PP-NE, with a polydispersity index of 0.249 and 0.198, respectively. Chemical and physiochemical analysis of TEM, SEM, XRD, zeta potential, drug loading capacity, and drug release measurements were done to confirm the synthesis and loading efficiencies of essential oils' active ingredients. At high concentrations of AJ and PP nanoemulsions (2000 ppm), O/W nanoemulsions showed higher larval mortality than both LDH conjugates and crude oils. The mortality rate reached 100% for 2nd and 4th instar larvae. The relative toxicities revealed that PP nanoemulsion (MA-NE) was the most effective larvicide, followed by AJ nanoemulsion (AJ-NE). There was a significant increase in defensive enzymes, phenoloxidase, and α and β-esterase enzymes in the treated groups. After treatment of L4 with AJ, AJ-NE, PP, and PP-NE, the levels of phenoloxidase were 545.67, 731.00, 700.00, and 799.67 u/mg, respectively, compared with control 669.67 u/mg. The activity levels of α-esterase were 9.71, 10.32, 8.91, and 10.55 mg α-naphthol/min/mg protein, respectively. It could be concluded that the AJ-NE and PP-NE nanoformulations have promising larvicidal activity and could act as safe and effective alternatives to chemical insecticides.https://doi.org/10.1038/s41598-024-56802-yJasminum sambacOil–water nanoemulsionEssential oil drug releaseMentha arvensis |
| spellingShingle | Ibrahim Taha Radwan Hanem F. Khater Shaimaa H. Mohammed Abdelwahab Khalil Mohamed A. Farghali Mohammed G. Mahmoud Abdelfattah Selim Eman A. Manaa Noha Bagato Mohamed M. Baz Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus Scientific Reports Jasminum sambac Oil–water nanoemulsion Essential oil drug release Mentha arvensis |
| title | Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus |
| title_full | Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus |
| title_fullStr | Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus |
| title_full_unstemmed | Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus |
| title_short | Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus |
| title_sort | synthesis of eco friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against culex pipiens linnaeus |
| topic | Jasminum sambac Oil–water nanoemulsion Essential oil drug release Mentha arvensis |
| url | https://doi.org/10.1038/s41598-024-56802-y |
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