Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress
Oxidative stress is one of the potential causes of nervous system disease. Ginseng extract possesses excellent antioxidant activity; however, little research on the function of the ginseng fibrous root. This study aimed to investigate the neuroprotective effects of ginseng fibrous root to alleviate...
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/7824 |
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| author | Yuhua Zhu Ziyan Wang Shuxuan Yu Chong Zhao Baofeng Xu Rui Liu Li Xu Yi Guo |
| author_facet | Yuhua Zhu Ziyan Wang Shuxuan Yu Chong Zhao Baofeng Xu Rui Liu Li Xu Yi Guo |
| author_sort | Yuhua Zhu |
| collection | DOAJ |
| description | Oxidative stress is one of the potential causes of nervous system disease. Ginseng extract possesses excellent antioxidant activity; however, little research on the function of the ginseng fibrous root. This study aimed to investigate the neuroprotective effects of ginseng fibrous root to alleviate the pathogenesis of Alzheimer’s disease (AD) against oxidative stress. Ginseng fibrous root enzymatic hydrolysate (GFREH) was first prepared by digesting ginseng fibrous roots with alkaline protease. In vitro, the GFREH showed antioxidant activities in free radical scavenging mechanisms. With a cellular model of AD, GFREH inhibited the increase in Ca<sup>2+</sup> levels and intracellular ROS content, maintained the balance of mitochondrial membrane potential, and relieved L-glutamic acid-induced neurotoxicity. In vivo, GFREH improved the survival rate of <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) under oxidative stress, upregulated SOD-3 expression, and reduced reactive oxygen species (ROS) content. Therefore, our findings provide evidence for the alleviation effect of GFREH against oxidative stress in neuroprotection, which may accelerate the development of anti-Alzheimer’s drugs and treatments in the future. |
| first_indexed | 2024-03-09T18:07:57Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:07:57Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6e59c765b33d4d6db2d22bbe5a932fa62023-11-24T09:21:52ZengMDPI AGMolecules1420-30492022-11-012722782410.3390/molecules27227824Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative StressYuhua Zhu0Ziyan Wang1Shuxuan Yu2Chong Zhao3Baofeng Xu4Rui Liu5Li Xu6Yi Guo7Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaDepartment of Neurosurgery, First Hospital of Jilin University, Changchun 130021, ChinaDepartment of VIP Unit, China-Japan Union Hospital of Jilin University, Changchun 130033, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaOxidative stress is one of the potential causes of nervous system disease. Ginseng extract possesses excellent antioxidant activity; however, little research on the function of the ginseng fibrous root. This study aimed to investigate the neuroprotective effects of ginseng fibrous root to alleviate the pathogenesis of Alzheimer’s disease (AD) against oxidative stress. Ginseng fibrous root enzymatic hydrolysate (GFREH) was first prepared by digesting ginseng fibrous roots with alkaline protease. In vitro, the GFREH showed antioxidant activities in free radical scavenging mechanisms. With a cellular model of AD, GFREH inhibited the increase in Ca<sup>2+</sup> levels and intracellular ROS content, maintained the balance of mitochondrial membrane potential, and relieved L-glutamic acid-induced neurotoxicity. In vivo, GFREH improved the survival rate of <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) under oxidative stress, upregulated SOD-3 expression, and reduced reactive oxygen species (ROS) content. Therefore, our findings provide evidence for the alleviation effect of GFREH against oxidative stress in neuroprotection, which may accelerate the development of anti-Alzheimer’s drugs and treatments in the future.https://www.mdpi.com/1420-3049/27/22/7824oxidative stressginsengantioxidantsAlzheimer’s diseaseneuroprotection |
| spellingShingle | Yuhua Zhu Ziyan Wang Shuxuan Yu Chong Zhao Baofeng Xu Rui Liu Li Xu Yi Guo Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress Molecules oxidative stress ginseng antioxidants Alzheimer’s disease neuroprotection |
| title | Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress |
| title_full | Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress |
| title_fullStr | Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress |
| title_full_unstemmed | Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress |
| title_short | Neuroprotective Effect of Ginseng Fibrous Root Enzymatic Hydrolysate against Oxidative Stress |
| title_sort | neuroprotective effect of ginseng fibrous root enzymatic hydrolysate against oxidative stress |
| topic | oxidative stress ginseng antioxidants Alzheimer’s disease neuroprotection |
| url | https://www.mdpi.com/1420-3049/27/22/7824 |
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