Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile
Probiotics might offer an attractive alternative to prevent and control Clostridium difficile (C. difficile) infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains to inhibit C. difficile. This study examined the anti-clostridial effects of Bifi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00888/full |
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| author | Yanxia Wei Fan Yang Qiong Wu Jing Gao Wenli Liu Chang Liu Xiaokui Guo Sharmila Suwal Yanbo Kou Bo Zhang Yugang Wang Kuiyang Zheng Renxian Tang |
| author_facet | Yanxia Wei Fan Yang Qiong Wu Jing Gao Wenli Liu Chang Liu Xiaokui Guo Sharmila Suwal Yanbo Kou Bo Zhang Yugang Wang Kuiyang Zheng Renxian Tang |
| author_sort | Yanxia Wei |
| collection | DOAJ |
| description | Probiotics might offer an attractive alternative to prevent and control Clostridium difficile (C. difficile) infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains to inhibit C. difficile. This study examined the anti-clostridial effects of Bifidobacterium longum JDM301, a widely used commercial probiotic strain in China, in vitro and in vivo. In vitro evaluation revealed a significant reduction in C. difficile counts when JDM301 was co-cultured with C. difficile, which was correlated with the significant decrease in clostridial toxin titres (TcdA and TcdB). Furthermore, the cell-free culture supernatants (CFS) of JDM301 inhibited C. difficile growth and degraded TcdA and TcdB. Notably, the results showed that acid pH promoted the degradation of TcdA by CFS from JDM301. Furthermore, comparative studies among 10 B. longum strains were performed, which showed that the inhibitory effect of CFS from JDM301 was similar with the other 8 B. longum strains and higher than strain BLY1. However, when it was neutralized, the significant different was lost. When present together, it was suggested that the acid pH induced by probiotics not only played important roles in the growth inhibition against C. difficile resulting in the reduction of toxins titres, but also directly promoted the degradation of clostridial toxin. In vivo studies proved that JDM301 partially relieved damage to tissues caused by C. difficile and also decreased the number of C. difficile and toxin levels. In summary, our results demonstrated that the commercial strain, JDM301 could be considered a probiotic able to exert anti-toxin capability and most of the CFS from Bifidobacterium were able to inhibit the growth of C. difficile, depending on acid pH. These results highlighted a potential that JDM301 could be helpful in preventing CDI and that most of the bifidobacterial strains could (at least partially) exert protective effects by reducing toxin titres through growth inhibition against toxigenic C. difficile. |
| first_indexed | 2024-12-10T15:58:12Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T15:58:12Z |
| publishDate | 2018-05-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-6e5de6767bad4c88b4a551f5eee3cf1a2022-12-22T01:42:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.00888345075Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficileYanxia Wei0Fan Yang1Qiong Wu2Jing Gao3Wenli Liu4Chang Liu5Xiaokui Guo6Sharmila Suwal7Yanbo Kou8Bo Zhang9Yugang Wang10Kuiyang Zheng11Renxian Tang12Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaDepartment of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaJiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, ChinaProbiotics might offer an attractive alternative to prevent and control Clostridium difficile (C. difficile) infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains to inhibit C. difficile. This study examined the anti-clostridial effects of Bifidobacterium longum JDM301, a widely used commercial probiotic strain in China, in vitro and in vivo. In vitro evaluation revealed a significant reduction in C. difficile counts when JDM301 was co-cultured with C. difficile, which was correlated with the significant decrease in clostridial toxin titres (TcdA and TcdB). Furthermore, the cell-free culture supernatants (CFS) of JDM301 inhibited C. difficile growth and degraded TcdA and TcdB. Notably, the results showed that acid pH promoted the degradation of TcdA by CFS from JDM301. Furthermore, comparative studies among 10 B. longum strains were performed, which showed that the inhibitory effect of CFS from JDM301 was similar with the other 8 B. longum strains and higher than strain BLY1. However, when it was neutralized, the significant different was lost. When present together, it was suggested that the acid pH induced by probiotics not only played important roles in the growth inhibition against C. difficile resulting in the reduction of toxins titres, but also directly promoted the degradation of clostridial toxin. In vivo studies proved that JDM301 partially relieved damage to tissues caused by C. difficile and also decreased the number of C. difficile and toxin levels. In summary, our results demonstrated that the commercial strain, JDM301 could be considered a probiotic able to exert anti-toxin capability and most of the CFS from Bifidobacterium were able to inhibit the growth of C. difficile, depending on acid pH. These results highlighted a potential that JDM301 could be helpful in preventing CDI and that most of the bifidobacterial strains could (at least partially) exert protective effects by reducing toxin titres through growth inhibition against toxigenic C. difficile.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00888/fullBifidobacteriumClostridium difficileprobioticsprotective effectToxin AToxin B |
| spellingShingle | Yanxia Wei Fan Yang Qiong Wu Jing Gao Wenli Liu Chang Liu Xiaokui Guo Sharmila Suwal Yanbo Kou Bo Zhang Yugang Wang Kuiyang Zheng Renxian Tang Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile Frontiers in Microbiology Bifidobacterium Clostridium difficile probiotics protective effect Toxin A Toxin B |
| title | Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile |
| title_full | Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile |
| title_fullStr | Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile |
| title_full_unstemmed | Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile |
| title_short | Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile |
| title_sort | protective effects of bifidobacterial strains against toxigenic clostridium difficile |
| topic | Bifidobacterium Clostridium difficile probiotics protective effect Toxin A Toxin B |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00888/full |
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