Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair
Osteoarthritis is caused by the cartilage destruction of the bones of the joint surfaces and structures that produce synovium fluid. Osteoarthritis treatment includes the use of surgical methods and non-surgical or maintenance treatments including knee orthoses, medical insoles with external edges,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-04-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2024-0007 |
| _version_ | 1797208505985269760 |
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| author | Ji Fengkun Zeng Xu Wang Zhendong Chen Hui Li Wenchao Li Haoyu |
| author_facet | Ji Fengkun Zeng Xu Wang Zhendong Chen Hui Li Wenchao Li Haoyu |
| author_sort | Ji Fengkun |
| collection | DOAJ |
| description | Osteoarthritis is caused by the cartilage destruction of the bones of the joint surfaces and structures that produce synovium fluid. Osteoarthritis treatment includes the use of surgical methods and non-surgical or maintenance treatments including knee orthoses, medical insoles with external edges, use of physiotherapy techniques, exercise, weight loss in obese people, and teaching the principles of joint care. The main goal of treatment in osteoarthritis of the knee is to ameliorate physical function, decrease pain, and reduce the progression of the disease, through correcting the knee alignment and reducing the varus torque. Previous studies have indicated that medicinal plants and herbal nanoparticles (NPs) have the best anti-inflammatory effects. β-Cyclodextrin is a cyclic carbohydrate molecule that is used as a host to prepare inclusion complexes. In this study, the synthesis of nickel NPs is based on β-cyclodextrin (NiBCD NPs) for accelerating the osteoarthritis articular cartilage repair. The FT-IR and XRD techniques confirmed the formula of NiO for the NiBCD NPs. The FE-SEM imaging shows a non-spherical structure for NiBCD NPs with a size of less than 100 nm. In EDX, the signals at the energy levels of 8.3, 7.5, and 0.87 keV are assigned for the electron migration of Ni Kβ, Ni Kα, and Ni Lα. Furthermore, the signals for the elements of oxygen and carbon of BCD appeared at 0.52 and 0.28 keV. The effectiveness of NiBCD NPs in promoting chondrogenesis was examined in orthopedic experiments using primary cultured chondrocytes. Subsequently, we determined the functional restoration following NiBCD NPs’ transplantation in a knee osteoarthritis articular cartilage injury model. We conducted histological, PCR, and Western blot assays. In the immunological analysis, the levels of MMPs, IL-1β, TNF-α, and p-p65 expression were found to be reduced by NiBCD NPs. This reduction may be attributed to the regulation of cellular redox homeostasis through Nrf2. Furthermore, our findings demonstrated the positive impact of NiBCD NPs on stimulating chondrogenesis in vitro. Notably, the NiBCD NPs’ application accelerated the recovery of injury-induced dysfunction. Additionally, the presence of NiBCD NPs at the injury site suppressed abnormal fibrosis and angiogenesis. The histological assay revealed the chondrocytes’ proliferation and increased cartilage matrix synthesis in the NiBCD NPs’ presence. |
| first_indexed | 2024-04-24T09:39:53Z |
| format | Article |
| id | doaj.art-6e631a3c810841519339812d82b2c870 |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-04-24T09:39:53Z |
| publishDate | 2024-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-6e631a3c810841519339812d82b2c8702024-04-15T07:41:10ZengDe GruyterOpen Chemistry2391-54202024-04-0122112708510.1515/chem-2024-0007Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repairJi Fengkun0Zeng Xu1Wang Zhendong2Chen Hui3Li Wenchao4Li Haoyu5Department of Pediatric, The Seventh Medical Center of PLA General Hospital, No. 5, Nanmencanghutong, Dongcheng District, Beijing, 100700, ChinaDepartment of Neurosurgery, The seventh Medical Center of PLA General Hospital, No. 5, Nanmencanghutong, Dongcheng District, Beijing, 100700, ChinaDepartment of Pediatric, The Seventh Medical Center of PLA General Hospital, No. 5, Nanmencanghutong, Dongcheng District, Beijing, 100700, ChinaDepartment of Pediatric, The Seventh Medical Center of PLA General Hospital, No. 5, Nanmencanghutong, Dongcheng District, Beijing, 100700, ChinaDepartment of Pediatric, The Seventh Medical Center of PLA General Hospital, No. 5, Nanmencanghutong, Dongcheng District, Beijing, 100700, ChinaDepartment of Pediatric Surgery, The First Medical Center of PLA General Hospital, No. 28, Fuxing Road, Haidian District, Beijing, 100853, ChinaOsteoarthritis is caused by the cartilage destruction of the bones of the joint surfaces and structures that produce synovium fluid. Osteoarthritis treatment includes the use of surgical methods and non-surgical or maintenance treatments including knee orthoses, medical insoles with external edges, use of physiotherapy techniques, exercise, weight loss in obese people, and teaching the principles of joint care. The main goal of treatment in osteoarthritis of the knee is to ameliorate physical function, decrease pain, and reduce the progression of the disease, through correcting the knee alignment and reducing the varus torque. Previous studies have indicated that medicinal plants and herbal nanoparticles (NPs) have the best anti-inflammatory effects. β-Cyclodextrin is a cyclic carbohydrate molecule that is used as a host to prepare inclusion complexes. In this study, the synthesis of nickel NPs is based on β-cyclodextrin (NiBCD NPs) for accelerating the osteoarthritis articular cartilage repair. The FT-IR and XRD techniques confirmed the formula of NiO for the NiBCD NPs. The FE-SEM imaging shows a non-spherical structure for NiBCD NPs with a size of less than 100 nm. In EDX, the signals at the energy levels of 8.3, 7.5, and 0.87 keV are assigned for the electron migration of Ni Kβ, Ni Kα, and Ni Lα. Furthermore, the signals for the elements of oxygen and carbon of BCD appeared at 0.52 and 0.28 keV. The effectiveness of NiBCD NPs in promoting chondrogenesis was examined in orthopedic experiments using primary cultured chondrocytes. Subsequently, we determined the functional restoration following NiBCD NPs’ transplantation in a knee osteoarthritis articular cartilage injury model. We conducted histological, PCR, and Western blot assays. In the immunological analysis, the levels of MMPs, IL-1β, TNF-α, and p-p65 expression were found to be reduced by NiBCD NPs. This reduction may be attributed to the regulation of cellular redox homeostasis through Nrf2. Furthermore, our findings demonstrated the positive impact of NiBCD NPs on stimulating chondrogenesis in vitro. Notably, the NiBCD NPs’ application accelerated the recovery of injury-induced dysfunction. Additionally, the presence of NiBCD NPs at the injury site suppressed abnormal fibrosis and angiogenesis. The histological assay revealed the chondrocytes’ proliferation and increased cartilage matrix synthesis in the NiBCD NPs’ presence.https://doi.org/10.1515/chem-2024-0007nickelβ-cyclodextrinosteoarthritisarticular cartilage repair |
| spellingShingle | Ji Fengkun Zeng Xu Wang Zhendong Chen Hui Li Wenchao Li Haoyu Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair Open Chemistry nickel β-cyclodextrin osteoarthritis articular cartilage repair |
| title | Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair |
| title_full | Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair |
| title_fullStr | Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair |
| title_full_unstemmed | Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair |
| title_short | Preparation of an inclusion complex of nickel-based β-cyclodextrin: Characterization and accelerating the osteoarthritis articular cartilage repair |
| title_sort | preparation of an inclusion complex of nickel based β cyclodextrin characterization and accelerating the osteoarthritis articular cartilage repair |
| topic | nickel β-cyclodextrin osteoarthritis articular cartilage repair |
| url | https://doi.org/10.1515/chem-2024-0007 |
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