Extracellular matrix protein composition dynamically changes during murine forelimb development
Summary: The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding ho...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224000592 |
| _version_ | 1797350608587456512 |
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| author | Kathryn R. Jacobson Aya M. Saleh Sarah N. Lipp Chengzhe Tian Audrey R. Watson Callan M. Luetkemeyer Alexander R. Ocken Sabrina L. Spencer Tamara L. Kinzer-Ursem Sarah Calve |
| author_facet | Kathryn R. Jacobson Aya M. Saleh Sarah N. Lipp Chengzhe Tian Audrey R. Watson Callan M. Luetkemeyer Alexander R. Ocken Sabrina L. Spencer Tamara L. Kinzer-Ursem Sarah Calve |
| author_sort | Kathryn R. Jacobson |
| collection | DOAJ |
| description | Summary: The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5–14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development. |
| first_indexed | 2024-03-08T12:47:21Z |
| format | Article |
| id | doaj.art-6e642685f7e54261a6510f758ebac7cd |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T12:47:21Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-6e642685f7e54261a6510f758ebac7cd2024-01-21T05:09:56ZengElsevieriScience2589-00422024-02-01272108838Extracellular matrix protein composition dynamically changes during murine forelimb developmentKathryn R. Jacobson0Aya M. Saleh1Sarah N. Lipp2Chengzhe Tian3Audrey R. Watson4Callan M. Luetkemeyer5Alexander R. Ocken6Sabrina L. Spencer7Tamara L. Kinzer-Ursem8Sarah Calve9Purdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, IN 47907, USA; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USAWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USAWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; The Indiana University Medical Scientist/Engineer Training Program, Indiana University, Indianapolis, IN 46202, USADepartment of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA; Research Center for Molecular Medicine (CEMM) of the Austrian Academy of Sciences, Vienna, AustriaDepartment of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USAPaul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USAWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USADepartment of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USAPurdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, IN 47907, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USAPurdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, IN 47907, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA; Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA; Corresponding authorSummary: The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5–14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.http://www.sciencedirect.com/science/article/pii/S2589004224000592Natural sciencesBiological sciencesDevelopmental biology |
| spellingShingle | Kathryn R. Jacobson Aya M. Saleh Sarah N. Lipp Chengzhe Tian Audrey R. Watson Callan M. Luetkemeyer Alexander R. Ocken Sabrina L. Spencer Tamara L. Kinzer-Ursem Sarah Calve Extracellular matrix protein composition dynamically changes during murine forelimb development iScience Natural sciences Biological sciences Developmental biology |
| title | Extracellular matrix protein composition dynamically changes during murine forelimb development |
| title_full | Extracellular matrix protein composition dynamically changes during murine forelimb development |
| title_fullStr | Extracellular matrix protein composition dynamically changes during murine forelimb development |
| title_full_unstemmed | Extracellular matrix protein composition dynamically changes during murine forelimb development |
| title_short | Extracellular matrix protein composition dynamically changes during murine forelimb development |
| title_sort | extracellular matrix protein composition dynamically changes during murine forelimb development |
| topic | Natural sciences Biological sciences Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224000592 |
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