Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia
Background and Objectives: Cryopreservation of sperm is a widely used technique to maintain and protect fertility men in certain conditions, such as infertility and malignancy treatments. The aim of this study was to determine the effects of cryopreservation on sperm functional and molecular paramet...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Qom University of Medical Sciences
2020-06-01
|
| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| Subjects: | |
| Online Access: | http://journal.muq.ac.ir/article-1-2786-en.html |
| _version_ | 1819067335164559360 |
|---|---|
| author | Elham Asa Rahim Ahmadi Minoo Mahmoodi Abdolreza Mohammadnia |
| author_facet | Elham Asa Rahim Ahmadi Minoo Mahmoodi Abdolreza Mohammadnia |
| author_sort | Elham Asa |
| collection | DOAJ |
| description | Background and Objectives: Cryopreservation of sperm is a widely used technique to maintain and protect fertility men in certain conditions, such as infertility and malignancy treatments. The aim of this study was to determine the effects of cryopreservation on sperm functional and molecular parameters in infertile men with asthenoteratozoospermia.
Methods: According to previous studies in this field, semen samples were collected from 20 people with asthenoteratozoospermia. A part of sample was first examined as a fresh sample before rapid freezing. In the following, the samples were freezed using rapid freezing protocol for 14 days in liquid nitrogen and then were thawed, and finally, the functional parameters were analyzed by sperm analysis method and DNA fragmentation was assessed using TUNEL test. Viability percentage was evaluated by eosin-nigrosin staining. Data were analyzed using paired t-test.
Results: Cryopreservation led to significant decrease in sperm motility, viability, concentration, and normal morphology (p < 0.001). DNA damage significantly increased during cryopreservation (p < 0.01).
Conclusion: Common cryopreservation process has detrimental effects on human sperm functional and molecular parameters and can reduce fertility potential. |
| first_indexed | 2024-12-21T16:16:37Z |
| format | Article |
| id | doaj.art-6e65e46399c446f884354b925b122214 |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-21T16:16:37Z |
| publishDate | 2020-06-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-6e65e46399c446f884354b925b1222142022-12-21T18:57:41ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752020-06-011436473Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with AsthenoteratozoospermiaElham Asa0Rahim Ahmadi1Minoo Mahmoodi2Abdolreza Mohammadnia3 Department of Biology, School of Basic Sciences, Hamadan Branch, Islamic Azad University Department of Biology, School of Basic Sciences, Hamadan Branch, Islamic Azad University Department of Biology, School of Basic Sciences, Hamadan Branch, Islamic Azad University Department of Biotechnology, School of Advanced Technology in Medicine, Shahid Beheshti University of Medical Sciences Background and Objectives: Cryopreservation of sperm is a widely used technique to maintain and protect fertility men in certain conditions, such as infertility and malignancy treatments. The aim of this study was to determine the effects of cryopreservation on sperm functional and molecular parameters in infertile men with asthenoteratozoospermia. Methods: According to previous studies in this field, semen samples were collected from 20 people with asthenoteratozoospermia. A part of sample was first examined as a fresh sample before rapid freezing. In the following, the samples were freezed using rapid freezing protocol for 14 days in liquid nitrogen and then were thawed, and finally, the functional parameters were analyzed by sperm analysis method and DNA fragmentation was assessed using TUNEL test. Viability percentage was evaluated by eosin-nigrosin staining. Data were analyzed using paired t-test. Results: Cryopreservation led to significant decrease in sperm motility, viability, concentration, and normal morphology (p < 0.001). DNA damage significantly increased during cryopreservation (p < 0.01). Conclusion: Common cryopreservation process has detrimental effects on human sperm functional and molecular parameters and can reduce fertility potential.http://journal.muq.ac.ir/article-1-2786-en.htmlcryopreservationasthenoteratozoospermiasemen qualitydna fragmentation. |
| spellingShingle | Elham Asa Rahim Ahmadi Minoo Mahmoodi Abdolreza Mohammadnia Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum cryopreservation asthenoteratozoospermia semen quality dna fragmentation. |
| title | Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia |
| title_full | Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia |
| title_fullStr | Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia |
| title_full_unstemmed | Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia |
| title_short | Determination of the Detrimental Effects of Cryopreservation Process on Sperm Functional and Molecular Parameters in Infertile Men with Asthenoteratozoospermia |
| title_sort | determination of the detrimental effects of cryopreservation process on sperm functional and molecular parameters in infertile men with asthenoteratozoospermia |
| topic | cryopreservation asthenoteratozoospermia semen quality dna fragmentation. |
| url | http://journal.muq.ac.ir/article-1-2786-en.html |
| work_keys_str_mv | AT elhamasa determinationofthedetrimentaleffectsofcryopreservationprocessonspermfunctionalandmolecularparametersininfertilemenwithasthenoteratozoospermia AT rahimahmadi determinationofthedetrimentaleffectsofcryopreservationprocessonspermfunctionalandmolecularparametersininfertilemenwithasthenoteratozoospermia AT minoomahmoodi determinationofthedetrimentaleffectsofcryopreservationprocessonspermfunctionalandmolecularparametersininfertilemenwithasthenoteratozoospermia AT abdolrezamohammadnia determinationofthedetrimentaleffectsofcryopreservationprocessonspermfunctionalandmolecularparametersininfertilemenwithasthenoteratozoospermia |