Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing
Lactate biosensor fabrication using a chitosan and crosslinker configuration realised solution mediation (K3Fe(CN)6) with lactate linear range 9.9 × 10−4 to 5.66×10−3 M, sensitivity 1.44×10−3C cm−2 mM−1 and LOD of 0.54 mM. Further development involved use of the heterocyclic quinoid species 1,10-phe...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053921000084 |
| _version_ | 1798026764137529344 |
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| author | Grace Halpin Karen Herdman Eithne Dempsey |
| author_facet | Grace Halpin Karen Herdman Eithne Dempsey |
| author_sort | Grace Halpin |
| collection | DOAJ |
| description | Lactate biosensor fabrication using a chitosan and crosslinker configuration realised solution mediation (K3Fe(CN)6) with lactate linear range 9.9 × 10−4 to 5.66×10−3 M, sensitivity 1.44×10−3C cm−2 mM−1 and LOD of 0.54 mM. Further development involved use of the heterocyclic quinoid species 1,10-phenanthroline-5,6-dione which acted as a proton and electron acceptor in relation to FADH2 cofactor regeneration. Graphite ink was formulated and utilised as an underlying conductive layer for LOx enzyme immobilisation and enzymatic polymerisation of 1,10-phenanthroline-5,6-dione at a GC electrode, resulting in a linear range of 0.74 – 2.44×10−3 M, sensitivity of 4.11×10−4C cm−2 mM−1 and LOD of 0.06 mM. Scanning electron and electrochemical scanning microscopy realised surface characterisation of the enzyme layers. |
| first_indexed | 2024-04-11T18:40:39Z |
| format | Article |
| id | doaj.art-6e6becb8572c4cdd860838ce9f8621bf |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-04-11T18:40:39Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-6e6becb8572c4cdd860838ce9f8621bf2022-12-22T04:08:59ZengElsevierSensors and Actuators Reports2666-05392021-11-013100032Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensingGrace Halpin0Karen Herdman1Eithne Dempsey2Department of Chemistry, Kathleen Lonsdale Institute for Human Health, Maynooth University, Maynooth Co., Kildare, IrelandDepartment of Chemistry, Kathleen Lonsdale Institute for Human Health, Maynooth University, Maynooth Co., Kildare, IrelandCorresponding author.; Department of Chemistry, Kathleen Lonsdale Institute for Human Health, Maynooth University, Maynooth Co., Kildare, IrelandLactate biosensor fabrication using a chitosan and crosslinker configuration realised solution mediation (K3Fe(CN)6) with lactate linear range 9.9 × 10−4 to 5.66×10−3 M, sensitivity 1.44×10−3C cm−2 mM−1 and LOD of 0.54 mM. Further development involved use of the heterocyclic quinoid species 1,10-phenanthroline-5,6-dione which acted as a proton and electron acceptor in relation to FADH2 cofactor regeneration. Graphite ink was formulated and utilised as an underlying conductive layer for LOx enzyme immobilisation and enzymatic polymerisation of 1,10-phenanthroline-5,6-dione at a GC electrode, resulting in a linear range of 0.74 – 2.44×10−3 M, sensitivity of 4.11×10−4C cm−2 mM−1 and LOD of 0.06 mM. Scanning electron and electrochemical scanning microscopy realised surface characterisation of the enzyme layers.http://www.sciencedirect.com/science/article/pii/S2666053921000084Lactic acidLactate oxidase1,10-Phenanthroline-5,6-dioneEnzymatic polymerisationScanning electrochemical microscopy |
| spellingShingle | Grace Halpin Karen Herdman Eithne Dempsey Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing Sensors and Actuators Reports Lactic acid Lactate oxidase 1,10-Phenanthroline-5,6-dione Enzymatic polymerisation Scanning electrochemical microscopy |
| title | Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing |
| title_full | Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing |
| title_fullStr | Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing |
| title_full_unstemmed | Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing |
| title_short | Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing |
| title_sort | electrochemical investigations into enzymatic polymerisation of 1 10 phenanthroline 5 6 dione as a redox mediator for lactate sensing |
| topic | Lactic acid Lactate oxidase 1,10-Phenanthroline-5,6-dione Enzymatic polymerisation Scanning electrochemical microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666053921000084 |
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