High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers
The recent availability of an assembled and annotated genome reference sequence for the diploid crop barley (Hordeum vulgare L.) provides new opportunities to study the genetic basis of agronomically important traits such as resistance to stripe [Puccinia striiformis f. sp. hordei (Psh)], leaf [P. h...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00467/full |
| _version_ | 1828389913721044992 |
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| author | Peter M. Dracatos Rouja Haghdoust Ravi P. Singh Ravi P. Singh Julio Huerta Espino Julio Huerta Espino Charles W. Barnes Kerrie Forrest Matthew Hayden Rients E. Niks Robert F. Park Davinder Singh |
| author_facet | Peter M. Dracatos Rouja Haghdoust Ravi P. Singh Ravi P. Singh Julio Huerta Espino Julio Huerta Espino Charles W. Barnes Kerrie Forrest Matthew Hayden Rients E. Niks Robert F. Park Davinder Singh |
| author_sort | Peter M. Dracatos |
| collection | DOAJ |
| description | The recent availability of an assembled and annotated genome reference sequence for the diploid crop barley (Hordeum vulgare L.) provides new opportunities to study the genetic basis of agronomically important traits such as resistance to stripe [Puccinia striiformis f. sp. hordei (Psh)], leaf [P. hordei (Ph)], and stem [P. graminis f. sp. tritici (Pgt)] rust diseases. The European barley cultivar Pompadour is known to possess high levels of resistance to leaf rust, predominantly due to adult plant resistance (APR) gene Rph20. We developed a barley recombinant inbred line (RIL) population from a cross between Pompadour and the leaf rust and stripe rust susceptible selection Biosaline-19 (B-19), and genotyped this population using DArT-Seq genotyping by sequencing (GBS) markers. In the current study, we produced a high-density linkage map comprising 8,610 (SNP and in silico) markers spanning 5957.6 cM, with the aim of mapping loci for resistance to leaf rust, stem rust, and stripe rust. The RIL population was phenotyped in the field with Psh (Mexico and Ecuador) and Ph (Australia) and in the greenhouse at the seedling stage with Australian Ph and Pgt races, and at Wageningen University with a European variant of Psh race 24 (PshWUR). For Psh, we identified a consistent field QTL on chromosome 2H across all South American field sites and years. Two complementary resistance genes were mapped to chromosomes 1H and 4H at the seedling stage in response to PshWUR, likely to be the loci rpsEm1 and rpsEm2 previously reported from the cultivar Emir from which Pompadour was bred. For leaf rust, we determined that Rph20 in addition to two minor-effect QTL on 1H and 3H were effective at the seedling stage, whilst seedling resistance to stem rust was due to QTL on chromosomes 3H and 7H conferred by Pompadour and B-19, respectively. |
| first_indexed | 2024-12-10T06:35:15Z |
| format | Article |
| id | doaj.art-6e6c0be9de5a4b7c922172c638f24d71 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T06:35:15Z |
| publishDate | 2019-04-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-6e6c0be9de5a4b7c922172c638f24d712022-12-22T01:58:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00467451318High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq MarkersPeter M. Dracatos0Rouja Haghdoust1Ravi P. Singh2Ravi P. Singh3Julio Huerta Espino4Julio Huerta Espino5Charles W. Barnes6Kerrie Forrest7Matthew Hayden8Rients E. Niks9Robert F. Park10Davinder Singh11Plant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, AustraliaPlant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, AustraliaInternational Maize and Wheat Improvement Center, Texcoco, MexicoCampo Experimental Valle de México, INIFAP, Chapingo, MexicoInternational Maize and Wheat Improvement Center, Texcoco, MexicoCampo Experimental Valle de México, INIFAP, Chapingo, MexicoInstituto Nacional de Investigaciones Agropecuarias (INIAP), Quito, EcuadorAgriculture Victoria, AgriBio, Centre for AgriBioscience, La Trobe University, Melbourne, VIC, AustraliaAgriculture Victoria, AgriBio, Centre for AgriBioscience, La Trobe University, Melbourne, VIC, AustraliaPlant Breeding, Wageningen University & Research, Wageningen, NetherlandsPlant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, AustraliaPlant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, AustraliaThe recent availability of an assembled and annotated genome reference sequence for the diploid crop barley (Hordeum vulgare L.) provides new opportunities to study the genetic basis of agronomically important traits such as resistance to stripe [Puccinia striiformis f. sp. hordei (Psh)], leaf [P. hordei (Ph)], and stem [P. graminis f. sp. tritici (Pgt)] rust diseases. The European barley cultivar Pompadour is known to possess high levels of resistance to leaf rust, predominantly due to adult plant resistance (APR) gene Rph20. We developed a barley recombinant inbred line (RIL) population from a cross between Pompadour and the leaf rust and stripe rust susceptible selection Biosaline-19 (B-19), and genotyped this population using DArT-Seq genotyping by sequencing (GBS) markers. In the current study, we produced a high-density linkage map comprising 8,610 (SNP and in silico) markers spanning 5957.6 cM, with the aim of mapping loci for resistance to leaf rust, stem rust, and stripe rust. The RIL population was phenotyped in the field with Psh (Mexico and Ecuador) and Ph (Australia) and in the greenhouse at the seedling stage with Australian Ph and Pgt races, and at Wageningen University with a European variant of Psh race 24 (PshWUR). For Psh, we identified a consistent field QTL on chromosome 2H across all South American field sites and years. Two complementary resistance genes were mapped to chromosomes 1H and 4H at the seedling stage in response to PshWUR, likely to be the loci rpsEm1 and rpsEm2 previously reported from the cultivar Emir from which Pompadour was bred. For leaf rust, we determined that Rph20 in addition to two minor-effect QTL on 1H and 3H were effective at the seedling stage, whilst seedling resistance to stem rust was due to QTL on chromosomes 3H and 7H conferred by Pompadour and B-19, respectively.https://www.frontiersin.org/article/10.3389/fpls.2019.00467/fullhigh-density linkage mapDArT-Seq markersrust resistanceQTLbarley |
| spellingShingle | Peter M. Dracatos Rouja Haghdoust Ravi P. Singh Ravi P. Singh Julio Huerta Espino Julio Huerta Espino Charles W. Barnes Kerrie Forrest Matthew Hayden Rients E. Niks Robert F. Park Davinder Singh High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers Frontiers in Plant Science high-density linkage map DArT-Seq markers rust resistance QTL barley |
| title | High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers |
| title_full | High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers |
| title_fullStr | High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers |
| title_full_unstemmed | High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers |
| title_short | High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers |
| title_sort | high density mapping of triple rust resistance in barley using dart seq markers |
| topic | high-density linkage map DArT-Seq markers rust resistance QTL barley |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00467/full |
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