Regulation of dynamic pigment cell states at single-cell resolution
Cells bearing pigment have diverse roles and are often under strict evolutionary selection. Here, we explore the regulation of pigmented cells in the purple sea urchin Strongylocentrotus purpuratus, an emerging model for diverse pigment function. We took advantage of single cell RNA-seq (scRNAseq) t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-08-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/60388 |
| _version_ | 1797261507003678720 |
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| author | Margherita Perillo Nathalie Oulhen Stephany Foster Maxwell Spurrell Cristina Calestani Gary Wessel |
| author_facet | Margherita Perillo Nathalie Oulhen Stephany Foster Maxwell Spurrell Cristina Calestani Gary Wessel |
| author_sort | Margherita Perillo |
| collection | DOAJ |
| description | Cells bearing pigment have diverse roles and are often under strict evolutionary selection. Here, we explore the regulation of pigmented cells in the purple sea urchin Strongylocentrotus purpuratus, an emerging model for diverse pigment function. We took advantage of single cell RNA-seq (scRNAseq) technology and discovered that pigment cells in the embryo segregated into two distinct populations, a mitotic cluster and a post-mitotic cluster. Gcm is essential for expression of several genes important for pigment function, but is only transiently expressed in these cells. We discovered unique genes expressed by pigment cells and test their expression with double fluorescence in situ hybridization. These genes include new members of the fmo family that are expressed selectively in pigment cells of the embryonic and in the coelomic cells of the adult - both cell-types having immune functions. Overall, this study identifies nodes of molecular intersection ripe for change by selective evolutionary pressures. |
| first_indexed | 2024-04-24T23:42:19Z |
| format | Article |
| id | doaj.art-6e6e8231a0794fa282f0d546d658dd64 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-24T23:42:19Z |
| publishDate | 2020-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-6e6e8231a0794fa282f0d546d658dd642024-03-15T11:36:56ZengeLife Sciences Publications LtdeLife2050-084X2020-08-01910.7554/eLife.60388Regulation of dynamic pigment cell states at single-cell resolutionMargherita Perillo0https://orcid.org/0000-0003-0845-507XNathalie Oulhen1Stephany Foster2https://orcid.org/0000-0002-9404-1692Maxwell Spurrell3https://orcid.org/0000-0001-7314-7482Cristina Calestani4https://orcid.org/0000-0001-8122-9349Gary Wessel5https://orcid.org/0000-0002-1210-9279Department of Molecular and Cellular Biology Division of Biology and Medicine Brown University, Providence, United StatesDepartment of Molecular and Cellular Biology Division of Biology and Medicine Brown University, Providence, United StatesDepartment of Molecular and Cellular Biology Division of Biology and Medicine Brown University, Providence, United StatesDepartment of Molecular and Cellular Biology Division of Biology and Medicine Brown University, Providence, United StatesDepartment of Biology Valdosta State University, Valdosta, United StatesDepartment of Molecular and Cellular Biology Division of Biology and Medicine Brown University, Providence, United StatesCells bearing pigment have diverse roles and are often under strict evolutionary selection. Here, we explore the regulation of pigmented cells in the purple sea urchin Strongylocentrotus purpuratus, an emerging model for diverse pigment function. We took advantage of single cell RNA-seq (scRNAseq) technology and discovered that pigment cells in the embryo segregated into two distinct populations, a mitotic cluster and a post-mitotic cluster. Gcm is essential for expression of several genes important for pigment function, but is only transiently expressed in these cells. We discovered unique genes expressed by pigment cells and test their expression with double fluorescence in situ hybridization. These genes include new members of the fmo family that are expressed selectively in pigment cells of the embryonic and in the coelomic cells of the adult - both cell-types having immune functions. Overall, this study identifies nodes of molecular intersection ripe for change by selective evolutionary pressures.https://elifesciences.org/articles/60388sea urchinpigment cellsscmRNA-seqgene regulatory networkinnate immunityglial cells missing |
| spellingShingle | Margherita Perillo Nathalie Oulhen Stephany Foster Maxwell Spurrell Cristina Calestani Gary Wessel Regulation of dynamic pigment cell states at single-cell resolution eLife sea urchin pigment cells scmRNA-seq gene regulatory network innate immunity glial cells missing |
| title | Regulation of dynamic pigment cell states at single-cell resolution |
| title_full | Regulation of dynamic pigment cell states at single-cell resolution |
| title_fullStr | Regulation of dynamic pigment cell states at single-cell resolution |
| title_full_unstemmed | Regulation of dynamic pigment cell states at single-cell resolution |
| title_short | Regulation of dynamic pigment cell states at single-cell resolution |
| title_sort | regulation of dynamic pigment cell states at single cell resolution |
| topic | sea urchin pigment cells scmRNA-seq gene regulatory network innate immunity glial cells missing |
| url | https://elifesciences.org/articles/60388 |
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