The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy
Abstract Background The Hippo signaling pathway is reported to be involved in angiogenesis, but the roles of the Hippo pathway in diabetic retinopathy have not been addressed. Fufang Xueshuantong Capsule has been used to treat diabetic retinopathy in China; however, the effect of Fufang Xueshuantong...
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1678-3 |
| _version_ | 1818973545207693312 |
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| author | Gai-mei Hao Tian-tian Lv Yan Wu Hong-liang Wang Wei Xing Yong Wang Chun Li Zi-jian Zhang Zheng-lin Wang Wei Wang Jing Han |
| author_facet | Gai-mei Hao Tian-tian Lv Yan Wu Hong-liang Wang Wei Xing Yong Wang Chun Li Zi-jian Zhang Zheng-lin Wang Wei Wang Jing Han |
| author_sort | Gai-mei Hao |
| collection | DOAJ |
| description | Abstract Background The Hippo signaling pathway is reported to be involved in angiogenesis, but the roles of the Hippo pathway in diabetic retinopathy have not been addressed. Fufang Xueshuantong Capsule has been used to treat diabetic retinopathy in China; however, the effect of Fufang Xueshuantong Capsule on the Hippo pathway has not been investigated. Methods In this study, diabetes was induced in Sprague-Dawley rats with intraperitoneal injection of streptozotocin. Twenty weeks later, Fufang Xueshuantong Capsule was administered for 12 weeks. When the administration ended, the eyes were isolated for western blot and immunohistochemistry analyses. The levels of P- mammalian sterile 20-like (MST), large tumor suppressor homolog (Lats), P- yes-associated protein (YAP), transcriptional co-activator with PDZ binding motif (TAZ) and TEA domain family members (TEAD) were measured. Results Diabetic rats had a decreased P-MST level in the inner plexiform layer and reduced expression of P-YAP in the photoreceptor layers of their eyes. In addition, diabetic rats displayed remarkable increases in Lats, TAZ and TEAD in their retinas. Furthermore, Fufang Xueshuantong Capsule restored the changes in the Hippo pathway. Conclusions The Hippo signaling pathway is important for the progression of diabetic retinopathy and will hopefully be a targeted therapeutic approach for the prevention of diabetic retinopathy. |
| first_indexed | 2024-12-20T15:25:52Z |
| format | Article |
| id | doaj.art-6e6e9a8eae714250953521b68fc1f88e |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-20T15:25:52Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-6e6e9a8eae714250953521b68fc1f88e2022-12-21T19:35:49ZengBMCBMC Complementary and Alternative Medicine1472-68822017-04-0117111010.1186/s12906-017-1678-3The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathyGai-mei Hao0Tian-tian Lv1Yan Wu2Hong-liang Wang3Wei Xing4Yong Wang5Chun Li6Zi-jian Zhang7Zheng-lin Wang8Wei Wang9Jing Han10College of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineInstitute of Chinese Medicine, Beijing University of Chinese medicineCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineModern Research Center for Traditional Chinese Medicine, Beijing University of Chinese MedicineInstitute of Chinese Medicine, Beijing University of Chinese medicineCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineCollege of Basic Medicine, Key Laboratory of Ministry of Education (Syndromes and formulas), Key Laboratory of Beijing (Syndromes and formulas), Beijing University of Chinese medicineInstitute of Chinese Medicine, Beijing University of Chinese medicineAbstract Background The Hippo signaling pathway is reported to be involved in angiogenesis, but the roles of the Hippo pathway in diabetic retinopathy have not been addressed. Fufang Xueshuantong Capsule has been used to treat diabetic retinopathy in China; however, the effect of Fufang Xueshuantong Capsule on the Hippo pathway has not been investigated. Methods In this study, diabetes was induced in Sprague-Dawley rats with intraperitoneal injection of streptozotocin. Twenty weeks later, Fufang Xueshuantong Capsule was administered for 12 weeks. When the administration ended, the eyes were isolated for western blot and immunohistochemistry analyses. The levels of P- mammalian sterile 20-like (MST), large tumor suppressor homolog (Lats), P- yes-associated protein (YAP), transcriptional co-activator with PDZ binding motif (TAZ) and TEA domain family members (TEAD) were measured. Results Diabetic rats had a decreased P-MST level in the inner plexiform layer and reduced expression of P-YAP in the photoreceptor layers of their eyes. In addition, diabetic rats displayed remarkable increases in Lats, TAZ and TEAD in their retinas. Furthermore, Fufang Xueshuantong Capsule restored the changes in the Hippo pathway. Conclusions The Hippo signaling pathway is important for the progression of diabetic retinopathy and will hopefully be a targeted therapeutic approach for the prevention of diabetic retinopathy.http://link.springer.com/article/10.1186/s12906-017-1678-3Diabetic retinopathyChinese medicineHippo |
| spellingShingle | Gai-mei Hao Tian-tian Lv Yan Wu Hong-liang Wang Wei Xing Yong Wang Chun Li Zi-jian Zhang Zheng-lin Wang Wei Wang Jing Han The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy BMC Complementary and Alternative Medicine Diabetic retinopathy Chinese medicine Hippo |
| title | The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy |
| title_full | The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy |
| title_fullStr | The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy |
| title_full_unstemmed | The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy |
| title_short | The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy |
| title_sort | hippo signaling pathway a potential therapeutic target is reversed by a chinese patent drug in rats with diabetic retinopathy |
| topic | Diabetic retinopathy Chinese medicine Hippo |
| url | http://link.springer.com/article/10.1186/s12906-017-1678-3 |
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