A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro
Fusion of mitochondrial outer membranes is crucial for proper organelle function and involves large GTPases called mitofusins. The discrete steps that allow mitochondria to attach to one another and merge their outer membranes are unknown. By combining an in vitro mitochondrial fusion assay with ele...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-06-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/14618 |
| _version_ | 1811180610699395072 |
|---|---|
| author | Tobias Brandt Laetitia Cavellini Werner Kühlbrandt Mickaël M Cohen |
| author_facet | Tobias Brandt Laetitia Cavellini Werner Kühlbrandt Mickaël M Cohen |
| author_sort | Tobias Brandt |
| collection | DOAJ |
| description | Fusion of mitochondrial outer membranes is crucial for proper organelle function and involves large GTPases called mitofusins. The discrete steps that allow mitochondria to attach to one another and merge their outer membranes are unknown. By combining an in vitro mitochondrial fusion assay with electron cryo-tomography (cryo-ET), we visualize the junction between attached mitochondria isolated from Saccharomyces cerevisiae and observe complexes that mediate this attachment. We find that cycles of GTP hydrolysis induce progressive formation of a docking ring structure around extended areas of contact. Further GTP hydrolysis triggers local outer membrane fusion at the periphery of the contact region. These findings unravel key features of mitofusin-dependent fusion of outer membranes and constitute an important advance in our understanding of how mitochondria connect and merge. |
| first_indexed | 2024-04-11T09:06:11Z |
| format | Article |
| id | doaj.art-6e6ed8bf146c451d9972f4d84f2e61cc |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:06:11Z |
| publishDate | 2016-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-6e6ed8bf146c451d9972f4d84f2e61cc2022-12-22T04:32:37ZengeLife Sciences Publications LtdeLife2050-084X2016-06-01510.7554/eLife.14618A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitroTobias Brandt0Laetitia Cavellini1Werner Kühlbrandt2Mickaël M Cohen3https://orcid.org/0000-0002-1372-680XMax Planck Institute of Biophysics, Frankfurt, GermanyLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Sorbonne Universités, Paris, FranceMax Planck Institute of Biophysics, Frankfurt, GermanyLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Sorbonne Universités, Paris, FranceFusion of mitochondrial outer membranes is crucial for proper organelle function and involves large GTPases called mitofusins. The discrete steps that allow mitochondria to attach to one another and merge their outer membranes are unknown. By combining an in vitro mitochondrial fusion assay with electron cryo-tomography (cryo-ET), we visualize the junction between attached mitochondria isolated from Saccharomyces cerevisiae and observe complexes that mediate this attachment. We find that cycles of GTP hydrolysis induce progressive formation of a docking ring structure around extended areas of contact. Further GTP hydrolysis triggers local outer membrane fusion at the periphery of the contact region. These findings unravel key features of mitofusin-dependent fusion of outer membranes and constitute an important advance in our understanding of how mitochondria connect and merge.https://elifesciences.org/articles/14618mitochondriamembrane fusionmitofusindynamin-related-proteinsmitochondrial dynamicscryo-EM |
| spellingShingle | Tobias Brandt Laetitia Cavellini Werner Kühlbrandt Mickaël M Cohen A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro eLife mitochondria membrane fusion mitofusin dynamin-related-proteins mitochondrial dynamics cryo-EM |
| title | A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro |
| title_full | A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro |
| title_fullStr | A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro |
| title_full_unstemmed | A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro |
| title_short | A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro |
| title_sort | mitofusin dependent docking ring complex triggers mitochondrial fusion in vitro |
| topic | mitochondria membrane fusion mitofusin dynamin-related-proteins mitochondrial dynamics cryo-EM |
| url | https://elifesciences.org/articles/14618 |
| work_keys_str_mv | AT tobiasbrandt amitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT laetitiacavellini amitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT wernerkuhlbrandt amitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT mickaelmcohen amitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT tobiasbrandt mitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT laetitiacavellini mitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT wernerkuhlbrandt mitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro AT mickaelmcohen mitofusindependentdockingringcomplextriggersmitochondrialfusioninvitro |