Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft
Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral cri...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/3/196 |
| _version_ | 1797608693946122240 |
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| author | Radina Vasileva Tzvetan Chaprazov |
| author_facet | Radina Vasileva Tzvetan Chaprazov |
| author_sort | Radina Vasileva |
| collection | DOAJ |
| description | Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone. |
| first_indexed | 2024-03-11T05:47:09Z |
| format | Article |
| id | doaj.art-6e6fc424961042158e524b652cb27796 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T05:47:09Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj.art-6e6fc424961042158e524b652cb277962023-11-17T14:20:38ZengMDPI AGVeterinary Sciences2306-73812023-03-0110319610.3390/vetsci10030196Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with XenograftRadina Vasileva0Tzvetan Chaprazov1Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaDepartment of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, BulgariaCritical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone.https://www.mdpi.com/2306-7381/10/3/196erythropoietinxenograftbone regenerationfemurrat |
| spellingShingle | Radina Vasileva Tzvetan Chaprazov Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft Veterinary Sciences erythropoietin xenograft bone regeneration femur rat |
| title | Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft |
| title_full | Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft |
| title_fullStr | Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft |
| title_full_unstemmed | Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft |
| title_short | Bone Healing of Critical-Sized Femoral Defects in Rats Treated with Erythropoietin Alone or in Combination with Xenograft |
| title_sort | bone healing of critical sized femoral defects in rats treated with erythropoietin alone or in combination with xenograft |
| topic | erythropoietin xenograft bone regeneration femur rat |
| url | https://www.mdpi.com/2306-7381/10/3/196 |
| work_keys_str_mv | AT radinavasileva bonehealingofcriticalsizedfemoraldefectsinratstreatedwitherythropoietinaloneorincombinationwithxenograft AT tzvetanchaprazov bonehealingofcriticalsizedfemoraldefectsinratstreatedwitherythropoietinaloneorincombinationwithxenograft |