Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation
Improving biomass production with the utilization of low-cost substrate is a crucial approach to overcome the hindrance of high cost in developing large-scale microalgae production. The microalga <i>Coelastrella</i> sp. KKU-P1 was mixotrophically cultivated using unhydrolyzed molasses as...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/8/3603 |
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| author | Kamolwan Thepsuthammarat Alissara Reungsang Pensri Plangklang |
| author_facet | Kamolwan Thepsuthammarat Alissara Reungsang Pensri Plangklang |
| author_sort | Kamolwan Thepsuthammarat |
| collection | DOAJ |
| description | Improving biomass production with the utilization of low-cost substrate is a crucial approach to overcome the hindrance of high cost in developing large-scale microalgae production. The microalga <i>Coelastrella</i> sp. KKU-P1 was mixotrophically cultivated using unhydrolyzed molasses as a carbon source, with the key environmental conditions being varied in order to maximize biomass production. The batch cultivation in flasks achieved the highest biomass production of 3.81 g/L, under an initial pH 5.0, a substrate to inoculum ratio of 100:3, an initial total sugar concentration of 10 g/L, and a sodium nitrate concentration of 1.5 g/L with continuous light illumination at 23.7 W/m<sup>2</sup>. The photobioreactor cultivation results indicated that CO<sub>2</sub> supplementation did not improve biomass production. An ambient concentration of CO<sub>2</sub> was sufficient to promote the mixotrophic growth of the microalga as indicated by the highest biomass production of 4.28 g/L with 33.91% protein, 46.71% carbohydrate, and 15.10% lipid. The results of the biochemical composition analysis suggest that the microalgal biomass obtained is promising as a source of essential amino acids and pigments as well as saturated and monounsaturated fatty acids. This research highlights the potential for bioresource production via microalgal mixotrophic cultivation using untreated molasses as a low-cost raw material. |
| first_indexed | 2024-03-11T04:41:31Z |
| format | Article |
| id | doaj.art-6e76e849756e48d68f48479f9ba497f5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:41:31Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6e76e849756e48d68f48479f9ba497f52023-11-17T20:41:21ZengMDPI AGMolecules1420-30492023-04-01288360310.3390/molecules28083603Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic CultivationKamolwan Thepsuthammarat0Alissara Reungsang1Pensri Plangklang2Graduate School, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandImproving biomass production with the utilization of low-cost substrate is a crucial approach to overcome the hindrance of high cost in developing large-scale microalgae production. The microalga <i>Coelastrella</i> sp. KKU-P1 was mixotrophically cultivated using unhydrolyzed molasses as a carbon source, with the key environmental conditions being varied in order to maximize biomass production. The batch cultivation in flasks achieved the highest biomass production of 3.81 g/L, under an initial pH 5.0, a substrate to inoculum ratio of 100:3, an initial total sugar concentration of 10 g/L, and a sodium nitrate concentration of 1.5 g/L with continuous light illumination at 23.7 W/m<sup>2</sup>. The photobioreactor cultivation results indicated that CO<sub>2</sub> supplementation did not improve biomass production. An ambient concentration of CO<sub>2</sub> was sufficient to promote the mixotrophic growth of the microalga as indicated by the highest biomass production of 4.28 g/L with 33.91% protein, 46.71% carbohydrate, and 15.10% lipid. The results of the biochemical composition analysis suggest that the microalgal biomass obtained is promising as a source of essential amino acids and pigments as well as saturated and monounsaturated fatty acids. This research highlights the potential for bioresource production via microalgal mixotrophic cultivation using untreated molasses as a low-cost raw material.https://www.mdpi.com/1420-3049/28/8/36033rd generation biomassheterothrophyphotoautotrophyamino acidsfatty acids |
| spellingShingle | Kamolwan Thepsuthammarat Alissara Reungsang Pensri Plangklang Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation Molecules 3rd generation biomass heterothrophy photoautotrophy amino acids fatty acids |
| title | Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation |
| title_full | Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation |
| title_fullStr | Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation |
| title_full_unstemmed | Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation |
| title_short | Microalga <i>Coelastrella</i> sp. Cultivation on Unhydrolyzed Molasses-Based Medium towards the Optimization of Conditions for Growth and Biomass Production under Mixotrophic Cultivation |
| title_sort | microalga i coelastrella i sp cultivation on unhydrolyzed molasses based medium towards the optimization of conditions for growth and biomass production under mixotrophic cultivation |
| topic | 3rd generation biomass heterothrophy photoautotrophy amino acids fatty acids |
| url | https://www.mdpi.com/1420-3049/28/8/3603 |
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