Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China
Analysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) is essential for the control and treatment of diseases caused by this important pathogen. In this study, MRSA isolates obtained from a tertiary caret hospital in...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-05-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00838/full |
| _version_ | 1811201789920280576 |
|---|---|
| author | Haishen Kong Haishen Kong Fei Yu Weili Zhang Xuefen Li Hongxia Wang |
| author_facet | Haishen Kong Haishen Kong Fei Yu Weili Zhang Xuefen Li Hongxia Wang |
| author_sort | Haishen Kong |
| collection | DOAJ |
| description | Analysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) is essential for the control and treatment of diseases caused by this important pathogen. In this study, MRSA isolates obtained from a tertiary caret hospital in China were subjected to spa typing, SCCmec typing, multiple locus sequence typing (MLST), and PCR targeting of the genes encoding Panton-Valentine leukocidin (PVL). The disk diffusion method was used to test the antimicrobial susceptibility of the isolates to 10 non-beta-lactam antibiotics. Among the 120 MRSA isolates studied, 18 spa types and 15 ST types were identified. The spa t311 type was the most common (a total of 60 isolates; 50%) among the study strains, and nearly all the t311 strains belonged to ST5, which is the most common ST type that was previously reported from China among the t002 isolates. ST5-II/t311 was the major prevalent clone (55, 45.8%), which was followed by ST5-II/t002 (12, 10.0%) and ST59-IV/t437 (11, 9.2%). PVL-encoding genes were found in 6.7% of the isolates. Although the ST5-II/t311 and ST5-II/t002 clones are different spa types, they shared the same resistance profile (clindamycin, erythromycin, and ciprofloxacin). Most isolates of the ST239-III/t037 clone were resistant to clindamycin, erythromycin, ciprofloxacin, gentamicin, tetracycline, and trimethoprim/sulfamethoxazole. By contrast, the MRSA isolates of the ST239-III/t030 clone were more resistant to rifampin, but they were susceptible to trimethoprim/sulfamethoxazole. Our data emphasize the need for ongoing epidemiologic surveillance. |
| first_indexed | 2024-04-12T02:26:33Z |
| format | Article |
| id | doaj.art-6e7b4481d29c40378129ed40cb6cffd2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T02:26:33Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6e7b4481d29c40378129ed40cb6cffd22022-12-22T03:51:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00838244745Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in ChinaHaishen Kong0Haishen Kong1Fei Yu2Weili Zhang3Xuefen Li4Hongxia Wang5State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, ChinaDepartment of Ultrasound, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, ChinaAnalysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) is essential for the control and treatment of diseases caused by this important pathogen. In this study, MRSA isolates obtained from a tertiary caret hospital in China were subjected to spa typing, SCCmec typing, multiple locus sequence typing (MLST), and PCR targeting of the genes encoding Panton-Valentine leukocidin (PVL). The disk diffusion method was used to test the antimicrobial susceptibility of the isolates to 10 non-beta-lactam antibiotics. Among the 120 MRSA isolates studied, 18 spa types and 15 ST types were identified. The spa t311 type was the most common (a total of 60 isolates; 50%) among the study strains, and nearly all the t311 strains belonged to ST5, which is the most common ST type that was previously reported from China among the t002 isolates. ST5-II/t311 was the major prevalent clone (55, 45.8%), which was followed by ST5-II/t002 (12, 10.0%) and ST59-IV/t437 (11, 9.2%). PVL-encoding genes were found in 6.7% of the isolates. Although the ST5-II/t311 and ST5-II/t002 clones are different spa types, they shared the same resistance profile (clindamycin, erythromycin, and ciprofloxacin). Most isolates of the ST239-III/t037 clone were resistant to clindamycin, erythromycin, ciprofloxacin, gentamicin, tetracycline, and trimethoprim/sulfamethoxazole. By contrast, the MRSA isolates of the ST239-III/t030 clone were more resistant to rifampin, but they were susceptible to trimethoprim/sulfamethoxazole. Our data emphasize the need for ongoing epidemiologic surveillance.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00838/fullmethicillin-resistant Staphylococcus aureuspvlgenotypingclonal complexesmultidrug resistance |
| spellingShingle | Haishen Kong Haishen Kong Fei Yu Weili Zhang Xuefen Li Hongxia Wang Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China Frontiers in Microbiology methicillin-resistant Staphylococcus aureus pvl genotyping clonal complexes multidrug resistance |
| title | Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China |
| title_full | Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China |
| title_fullStr | Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China |
| title_full_unstemmed | Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China |
| title_short | Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China |
| title_sort | molecular epidemiology and antibiotic resistance profiles of methicillin resistant staphylococcus aureus strains in a tertiary hospital in china |
| topic | methicillin-resistant Staphylococcus aureus pvl genotyping clonal complexes multidrug resistance |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00838/full |
| work_keys_str_mv | AT haishenkong molecularepidemiologyandantibioticresistanceprofilesofmethicillinresistantstaphylococcusaureusstrainsinatertiaryhospitalinchina AT haishenkong molecularepidemiologyandantibioticresistanceprofilesofmethicillinresistantstaphylococcusaureusstrainsinatertiaryhospitalinchina AT feiyu molecularepidemiologyandantibioticresistanceprofilesofmethicillinresistantstaphylococcusaureusstrainsinatertiaryhospitalinchina AT weilizhang molecularepidemiologyandantibioticresistanceprofilesofmethicillinresistantstaphylococcusaureusstrainsinatertiaryhospitalinchina AT xuefenli molecularepidemiologyandantibioticresistanceprofilesofmethicillinresistantstaphylococcusaureusstrainsinatertiaryhospitalinchina AT hongxiawang molecularepidemiologyandantibioticresistanceprofilesofmethicillinresistantstaphylococcusaureusstrainsinatertiaryhospitalinchina |