Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications
Lack of suitable auto/allografts has been delaying surgical interventions for the treatment of numerous disorders and has also caused a serious threat to public health. Tissue engineering could be one of the best alternatives to solve this issue. However, deficiency of oxygen supply in the wounded a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/12/6/1233 |
| _version_ | 1797566737201233920 |
|---|---|
| author | Turdimuhammad Abdullah Kalamegam Gauthaman Ahmed H. Hammad Kasturi Joshi Navare Ahmed A. Alshahrie Sidi A. Bencherif Ali Tamayol Adnan Memic |
| author_facet | Turdimuhammad Abdullah Kalamegam Gauthaman Ahmed H. Hammad Kasturi Joshi Navare Ahmed A. Alshahrie Sidi A. Bencherif Ali Tamayol Adnan Memic |
| author_sort | Turdimuhammad Abdullah |
| collection | DOAJ |
| description | Lack of suitable auto/allografts has been delaying surgical interventions for the treatment of numerous disorders and has also caused a serious threat to public health. Tissue engineering could be one of the best alternatives to solve this issue. However, deficiency of oxygen supply in the wounded and implanted engineered tissues, caused by circulatory problems and insufficient angiogenesis, has been a rate-limiting step in translation of tissue-engineered grafts. To address this issue, we designed oxygen-releasing electrospun composite scaffolds, based on a previously developed hybrid polymeric matrix composed of poly(glycerol sebacate) (PGS) and poly(<i>ε</i>-caprolactone) (PCL). By performing ball-milling, we were able to embed a large percent of calcium peroxide (CP) nanoparticles into the PGS/PCL nanofibers able to generate oxygen. The composite scaffold exhibited a smooth fiber structure, while providing sustainable oxygen release for several days to a week, and significantly improved cell metabolic activity due to alleviation of hypoxic environment around primary bone-marrow-derived mesenchymal stem cells (BM-MSCs). Moreover, the composite scaffolds also showed good antibacterial performance. In conjunction to other improved features, such as degradation behavior, the developed scaffolds are promising biomaterials for various tissue-engineering and wound-healing applications. |
| first_indexed | 2024-03-10T19:30:39Z |
| format | Article |
| id | doaj.art-6e7e40ea20f04240898fff04f58be2c0 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T19:30:39Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-6e7e40ea20f04240898fff04f58be2c02023-11-20T02:07:24ZengMDPI AGPolymers2073-43602020-05-01126123310.3390/polym12061233Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering ApplicationsTurdimuhammad Abdullah0Kalamegam Gauthaman1Ahmed H. Hammad2Kasturi Joshi Navare3Ahmed A. Alshahrie4Sidi A. Bencherif5Ali Tamayol6Adnan Memic7Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCenter of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCenter of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Chemical Engineering, Northeastern University, Boston, MA 02115, USACenter of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Bioengineering, Northeastern University, Boston, MA 02115, USADepartment of Biomedical Engineering, University of Connecticut, Farmington, CT 06030, USACenter of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi ArabiaLack of suitable auto/allografts has been delaying surgical interventions for the treatment of numerous disorders and has also caused a serious threat to public health. Tissue engineering could be one of the best alternatives to solve this issue. However, deficiency of oxygen supply in the wounded and implanted engineered tissues, caused by circulatory problems and insufficient angiogenesis, has been a rate-limiting step in translation of tissue-engineered grafts. To address this issue, we designed oxygen-releasing electrospun composite scaffolds, based on a previously developed hybrid polymeric matrix composed of poly(glycerol sebacate) (PGS) and poly(<i>ε</i>-caprolactone) (PCL). By performing ball-milling, we were able to embed a large percent of calcium peroxide (CP) nanoparticles into the PGS/PCL nanofibers able to generate oxygen. The composite scaffold exhibited a smooth fiber structure, while providing sustainable oxygen release for several days to a week, and significantly improved cell metabolic activity due to alleviation of hypoxic environment around primary bone-marrow-derived mesenchymal stem cells (BM-MSCs). Moreover, the composite scaffolds also showed good antibacterial performance. In conjunction to other improved features, such as degradation behavior, the developed scaffolds are promising biomaterials for various tissue-engineering and wound-healing applications.https://www.mdpi.com/2073-4360/12/6/1233oxygen-releasing scaffoldPGS/PCLcalcium peroxideelectrospinningbiodegradabilityantibacterial properties |
| spellingShingle | Turdimuhammad Abdullah Kalamegam Gauthaman Ahmed H. Hammad Kasturi Joshi Navare Ahmed A. Alshahrie Sidi A. Bencherif Ali Tamayol Adnan Memic Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications Polymers oxygen-releasing scaffold PGS/PCL calcium peroxide electrospinning biodegradability antibacterial properties |
| title | Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications |
| title_full | Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications |
| title_fullStr | Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications |
| title_full_unstemmed | Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications |
| title_short | Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications |
| title_sort | oxygen releasing antibacterial nanofibrous scaffolds for tissue engineering applications |
| topic | oxygen-releasing scaffold PGS/PCL calcium peroxide electrospinning biodegradability antibacterial properties |
| url | https://www.mdpi.com/2073-4360/12/6/1233 |
| work_keys_str_mv | AT turdimuhammadabdullah oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT kalamegamgauthaman oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT ahmedhhammad oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT kasturijoshinavare oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT ahmedaalshahrie oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT sidiabencherif oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT alitamayol oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications AT adnanmemic oxygenreleasingantibacterialnanofibrousscaffoldsfortissueengineeringapplications |