High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial
Reducing the alimentary purine intake contributes to the prevention of purine (especially xanthine) urolith formation, a common adverse effect of allopurinol treatment in dogs with <i>Leishmania</i> infections. Analyses of the purine content are not required in order to advertise a diet...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/19/3060 |
| _version_ | 1827722886845038592 |
|---|---|
| author | Melanie Kaempfle Michèle Bergmann Petra Koelle Katrin Hartmann |
| author_facet | Melanie Kaempfle Michèle Bergmann Petra Koelle Katrin Hartmann |
| author_sort | Melanie Kaempfle |
| collection | DOAJ |
| description | Reducing the alimentary purine intake contributes to the prevention of purine (especially xanthine) urolith formation, a common adverse effect of allopurinol treatment in dogs with <i>Leishmania</i> infections. Analyses of the purine content are not required in order to advertise a diet as low in purine. Due to different analytical methods, data provided on purine content are barely comparable. The aim of this study was to investigate the total purine content of 12 different dog diets. For this, the purine bases adenine, guanine, xanthine, and hypoxanthine were determined by standardised high performance liquid chromatography in commercially available urinary diets (n = 4), kidney diets (n = 2), low protein diets (n = 3), 1 vegan diet, 1 regular diet for healthy adult dogs, and 1 homemade low purine diet. Total purine amounts ranged between 10.2 and 90.9 mg/100 g of dry matter. The daily purine intake calculated for a 20 kg standard dog with the analysed diets ranged between 21.9 and 174.7 mg. The lowest daily purine intakes were achieved by 2 urinary urate diets, followed by the homemade diet. Differences in the purine content of commercially available diets need to be considered. Awareness has to be raised when selecting diets for dogs with <i>Leishmania</i> infections during allopurinol treatment in order to minimise the risk of urolith formation. |
| first_indexed | 2024-03-10T21:50:36Z |
| format | Article |
| id | doaj.art-6e8035b8a0214b04b7c75d2cf133800c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T21:50:36Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6e8035b8a0214b04b7c75d2cf133800c2023-11-19T13:59:40ZengMDPI AGAnimals2076-26152023-09-011319306010.3390/ani13193060High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot TrialMelanie Kaempfle0Michèle Bergmann1Petra Koelle2Katrin Hartmann3LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, GermanyLMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, GermanyLMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, GermanyLMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, GermanyReducing the alimentary purine intake contributes to the prevention of purine (especially xanthine) urolith formation, a common adverse effect of allopurinol treatment in dogs with <i>Leishmania</i> infections. Analyses of the purine content are not required in order to advertise a diet as low in purine. Due to different analytical methods, data provided on purine content are barely comparable. The aim of this study was to investigate the total purine content of 12 different dog diets. For this, the purine bases adenine, guanine, xanthine, and hypoxanthine were determined by standardised high performance liquid chromatography in commercially available urinary diets (n = 4), kidney diets (n = 2), low protein diets (n = 3), 1 vegan diet, 1 regular diet for healthy adult dogs, and 1 homemade low purine diet. Total purine amounts ranged between 10.2 and 90.9 mg/100 g of dry matter. The daily purine intake calculated for a 20 kg standard dog with the analysed diets ranged between 21.9 and 174.7 mg. The lowest daily purine intakes were achieved by 2 urinary urate diets, followed by the homemade diet. Differences in the purine content of commercially available diets need to be considered. Awareness has to be raised when selecting diets for dogs with <i>Leishmania</i> infections during allopurinol treatment in order to minimise the risk of urolith formation.https://www.mdpi.com/2076-2615/13/19/3060leishmaniosislow purinecaninedog dieturolithiasisxanthine |
| spellingShingle | Melanie Kaempfle Michèle Bergmann Petra Koelle Katrin Hartmann High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial Animals leishmaniosis low purine canine dog diet urolithiasis xanthine |
| title | High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial |
| title_full | High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial |
| title_fullStr | High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial |
| title_full_unstemmed | High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial |
| title_short | High Performance Liquid Chromatography Analysis and Description of Purine Content of Diets Suitable for Dogs with <i>Leishmania</i> Infection during Allopurinol Treatment—A Pilot Trial |
| title_sort | high performance liquid chromatography analysis and description of purine content of diets suitable for dogs with i leishmania i infection during allopurinol treatment a pilot trial |
| topic | leishmaniosis low purine canine dog diet urolithiasis xanthine |
| url | https://www.mdpi.com/2076-2615/13/19/3060 |
| work_keys_str_mv | AT melaniekaempfle highperformanceliquidchromatographyanalysisanddescriptionofpurinecontentofdietssuitablefordogswithileishmaniaiinfectionduringallopurinoltreatmentapilottrial AT michelebergmann highperformanceliquidchromatographyanalysisanddescriptionofpurinecontentofdietssuitablefordogswithileishmaniaiinfectionduringallopurinoltreatmentapilottrial AT petrakoelle highperformanceliquidchromatographyanalysisanddescriptionofpurinecontentofdietssuitablefordogswithileishmaniaiinfectionduringallopurinoltreatmentapilottrial AT katrinhartmann highperformanceliquidchromatographyanalysisanddescriptionofpurinecontentofdietssuitablefordogswithileishmaniaiinfectionduringallopurinoltreatmentapilottrial |