Atg11 tethers Atg9 vesicles to initiate selective autophagy.
Autophagy recycles cytoplasmic components by sequestering them in double membrane-surrounded autophagosomes. The two proteins Atg11 and Atg17 are scaffolding components of the Atg1 kinase complex. Atg17 recruits and tethers Atg9-donor vesicles, and the corresponding Atg1 kinase complex induces the f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-07-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3000377 |
| _version_ | 1831759813015502848 |
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| author | Nena Matscheko Peter Mayrhofer Yijian Rao Viola Beier Thomas Wollert |
| author_facet | Nena Matscheko Peter Mayrhofer Yijian Rao Viola Beier Thomas Wollert |
| author_sort | Nena Matscheko |
| collection | DOAJ |
| description | Autophagy recycles cytoplasmic components by sequestering them in double membrane-surrounded autophagosomes. The two proteins Atg11 and Atg17 are scaffolding components of the Atg1 kinase complex. Atg17 recruits and tethers Atg9-donor vesicles, and the corresponding Atg1 kinase complex induces the formation of nonselective autophagosomes. Atg11 initiates selective autophagy and coordinates the switch to nonselective autophagy by recruiting Atg17. The molecular function of Atg11 remained, however, less well understood. Here, we demonstrate that Atg11 is activated by cargo through a direct interaction with autophagy receptors. Activated Atg11 dimerizes and tethers Atg9 vesicles, which leads to the nucleation of phagophores in direct vicinity of cargo. Starvation reciprocally regulates the activity of both tethering factors by initiating the degradation of Atg11 while Atg17 is activated. This allows Atg17 to sequester and tether Atg9 vesicles independent of cargo to nucleate nonselective phagophores. Our data reveal insights into the molecular mechanisms governing cargo selection and specificity in autophagy. |
| first_indexed | 2024-12-22T03:38:45Z |
| format | Article |
| id | doaj.art-6e80543085e64893bc99d1dac9ec4fed |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-22T03:38:45Z |
| publishDate | 2019-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-6e80543085e64893bc99d1dac9ec4fed2022-12-21T18:40:18ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852019-07-01177e300037710.1371/journal.pbio.3000377Atg11 tethers Atg9 vesicles to initiate selective autophagy.Nena MatschekoPeter MayrhoferYijian RaoViola BeierThomas WollertAutophagy recycles cytoplasmic components by sequestering them in double membrane-surrounded autophagosomes. The two proteins Atg11 and Atg17 are scaffolding components of the Atg1 kinase complex. Atg17 recruits and tethers Atg9-donor vesicles, and the corresponding Atg1 kinase complex induces the formation of nonselective autophagosomes. Atg11 initiates selective autophagy and coordinates the switch to nonselective autophagy by recruiting Atg17. The molecular function of Atg11 remained, however, less well understood. Here, we demonstrate that Atg11 is activated by cargo through a direct interaction with autophagy receptors. Activated Atg11 dimerizes and tethers Atg9 vesicles, which leads to the nucleation of phagophores in direct vicinity of cargo. Starvation reciprocally regulates the activity of both tethering factors by initiating the degradation of Atg11 while Atg17 is activated. This allows Atg17 to sequester and tether Atg9 vesicles independent of cargo to nucleate nonselective phagophores. Our data reveal insights into the molecular mechanisms governing cargo selection and specificity in autophagy.https://doi.org/10.1371/journal.pbio.3000377 |
| spellingShingle | Nena Matscheko Peter Mayrhofer Yijian Rao Viola Beier Thomas Wollert Atg11 tethers Atg9 vesicles to initiate selective autophagy. PLoS Biology |
| title | Atg11 tethers Atg9 vesicles to initiate selective autophagy. |
| title_full | Atg11 tethers Atg9 vesicles to initiate selective autophagy. |
| title_fullStr | Atg11 tethers Atg9 vesicles to initiate selective autophagy. |
| title_full_unstemmed | Atg11 tethers Atg9 vesicles to initiate selective autophagy. |
| title_short | Atg11 tethers Atg9 vesicles to initiate selective autophagy. |
| title_sort | atg11 tethers atg9 vesicles to initiate selective autophagy |
| url | https://doi.org/10.1371/journal.pbio.3000377 |
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