Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice
Abstract Background Cell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes, not only in the standard but also in changing environmental conditions. In the past, this strategy has been extensively developed in pla...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-04-01
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| Series: | Rice |
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| Online Access: | http://link.springer.com/article/10.1186/s12284-018-0209-2 |
| _version_ | 1811203770688733184 |
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| author | Thi Thu Huyen Chu Thi Giang Hoang Duy Chi Trinh Charlotte Bureau Donaldo Meynard Aurore Vernet Mathieu Ingouff Nang Vinh Do Christophe Périn Emmanuel Guiderdoni Pascal Gantet Christophe Maurel Doan-Trung Luu |
| author_facet | Thi Thu Huyen Chu Thi Giang Hoang Duy Chi Trinh Charlotte Bureau Donaldo Meynard Aurore Vernet Mathieu Ingouff Nang Vinh Do Christophe Périn Emmanuel Guiderdoni Pascal Gantet Christophe Maurel Doan-Trung Luu |
| author_sort | Thi Thu Huyen Chu |
| collection | DOAJ |
| description | Abstract Background Cell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes, not only in the standard but also in changing environmental conditions. In the past, this strategy has been extensively developed in plant models such as Arabidopsis. Results Here, we generated a set of transgenic lines expressing membrane protein markers to extend this approach to rice, one of the most cultivated crop in the world and an emerging plant model. Lines expressing individually eight membrane protein markers including five aquaporins (OsPIP1;1, OsPIP2;4, OsPIP2;5, OsTIP1;1, OsTIP2;2) and three endosomal trafficking proteins (OsRab5a, OsGAP1, OsSCAMP1) were obtained. Importantly, we challenged in roots the aquaporin-expressing transgenic lines upon salt and osmotic stress and uncovered a highly dynamic behaviour of cell membrane. Conclusion We have uncovered the relocalization and dynamics of plasma membrane aquaporins upon salt and osmotic stresses in rice. Importantly, our data support a model where relocalization of OsPIPs is concomitant with their high cycling dynamics. |
| first_indexed | 2024-04-12T03:00:44Z |
| format | Article |
| id | doaj.art-6e812de8ed084a27a9804a17b1b3367d |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-04-12T03:00:44Z |
| publishDate | 2018-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-6e812de8ed084a27a9804a17b1b3367d2022-12-22T03:50:40ZengSpringerOpenRice1939-84251939-84332018-04-011111810.1186/s12284-018-0209-2Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in riceThi Thu Huyen Chu0Thi Giang Hoang1Duy Chi Trinh2Charlotte Bureau3Donaldo Meynard4Aurore Vernet5Mathieu Ingouff6Nang Vinh Do7Christophe Périn8Emmanuel Guiderdoni9Pascal Gantet10Christophe Maurel11Doan-Trung Luu12BPMP, Univ Montpellier, CNRS, INRA, SupAgroNational key laboratory for Plant Cell Biotechnology, Agricultural Genetics InstituteBPMP, Univ Montpellier, CNRS, INRA, SupAgroCIRAD, UMR AGAPCIRAD, UMR AGAPCIRAD, UMR AGAPUniversité de Montpellier, UMR DIADENational key laboratory for Plant Cell Biotechnology, Agricultural Genetics InstituteCIRAD, UMR AGAPCIRAD, UMR AGAPUniversity of Science and Technology of Hanoi, LMI RICEBPMP, Univ Montpellier, CNRS, INRA, SupAgroBPMP, Univ Montpellier, CNRS, INRA, SupAgroAbstract Background Cell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes, not only in the standard but also in changing environmental conditions. In the past, this strategy has been extensively developed in plant models such as Arabidopsis. Results Here, we generated a set of transgenic lines expressing membrane protein markers to extend this approach to rice, one of the most cultivated crop in the world and an emerging plant model. Lines expressing individually eight membrane protein markers including five aquaporins (OsPIP1;1, OsPIP2;4, OsPIP2;5, OsTIP1;1, OsTIP2;2) and three endosomal trafficking proteins (OsRab5a, OsGAP1, OsSCAMP1) were obtained. Importantly, we challenged in roots the aquaporin-expressing transgenic lines upon salt and osmotic stress and uncovered a highly dynamic behaviour of cell membrane. Conclusion We have uncovered the relocalization and dynamics of plasma membrane aquaporins upon salt and osmotic stresses in rice. Importantly, our data support a model where relocalization of OsPIPs is concomitant with their high cycling dynamics.http://link.springer.com/article/10.1186/s12284-018-0209-2Oryza sativaSubcellular markersIntracellular dynamicsAbiotic stress |
| spellingShingle | Thi Thu Huyen Chu Thi Giang Hoang Duy Chi Trinh Charlotte Bureau Donaldo Meynard Aurore Vernet Mathieu Ingouff Nang Vinh Do Christophe Périn Emmanuel Guiderdoni Pascal Gantet Christophe Maurel Doan-Trung Luu Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice Rice Oryza sativa Subcellular markers Intracellular dynamics Abiotic stress |
| title | Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice |
| title_full | Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice |
| title_fullStr | Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice |
| title_full_unstemmed | Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice |
| title_short | Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice |
| title_sort | sub cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice |
| topic | Oryza sativa Subcellular markers Intracellular dynamics Abiotic stress |
| url | http://link.springer.com/article/10.1186/s12284-018-0209-2 |
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