Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS
Bulbil germination is crucial to the survival of Chinese yam plants, the preservation of germplasm resources and the worldwide supply of food and natural medicine. There are still some unknowns regarding bulbil biochemical variations associated with germination. The metabolic changes during the ger...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2024-02-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5247 |
| _version_ | 1797257271634296832 |
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| author | Xiaojin Ge Xiangyang Li Dandan Dai Zhen Yang Yanhong Wang Tiegang Yang Guixiao La |
| author_facet | Xiaojin Ge Xiangyang Li Dandan Dai Zhen Yang Yanhong Wang Tiegang Yang Guixiao La |
| author_sort | Xiaojin Ge |
| collection | DOAJ |
| description |
Bulbil germination is crucial to the survival of Chinese yam plants, the preservation of germplasm resources and the worldwide supply of food and natural medicine. There are still some unknowns regarding bulbil biochemical variations associated with germination. The metabolic changes during the germination of Chinese yam (Dioscorea polystachya Turczaninow) bulbils were studied using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) at eight-time points covering all four phases of germination. It was determined that 27 metabolites, including organic acids, amino acids, sugars, lipid metabolites, phenolics and steroids, were responsible for the variation in the Chinese yam bulbil groups. A metabolomics pathway was proposed based on the identified metabolites. The main processes affected during germination were those related to carbohydrate metabolism, the TCA cycle, lipid metabolism, nitrogen metabolism, lipid metabolism and polyphenol metabolism. It is one of the earliest reports on the metabolite identification and profiling of Chinese yam bulbils at different germination stages.
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| first_indexed | 2024-04-24T22:34:59Z |
| format | Article |
| id | doaj.art-6e8326177c8e4d189ae4eecd698478f3 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-04-24T22:34:59Z |
| publishDate | 2024-02-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-6e8326177c8e4d189ae4eecd698478f32024-03-19T11:28:54ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052024-02-0123110.24326/asphc.2024.5247Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MSXiaojin Ge0Xiangyang Li1Dandan Dai2Zhen Yang3Yanhong Wang4Tiegang Yang5Guixiao La6Institute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. ChinaInstitute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. ChinaInstitute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. ChinaXixian Agricultural Science Research InstituteInstitute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. ChinaInstitute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. ChinaInstitute of Chinese Herbel Medicines, Henan Academy of Agricultural Sciences; Henan Provincial Center for Engineering and Technology Research of Traditional Chinese Medicine, Zhengzhou, Henan 450046, P.R. China Bulbil germination is crucial to the survival of Chinese yam plants, the preservation of germplasm resources and the worldwide supply of food and natural medicine. There are still some unknowns regarding bulbil biochemical variations associated with germination. The metabolic changes during the germination of Chinese yam (Dioscorea polystachya Turczaninow) bulbils were studied using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) at eight-time points covering all four phases of germination. It was determined that 27 metabolites, including organic acids, amino acids, sugars, lipid metabolites, phenolics and steroids, were responsible for the variation in the Chinese yam bulbil groups. A metabolomics pathway was proposed based on the identified metabolites. The main processes affected during germination were those related to carbohydrate metabolism, the TCA cycle, lipid metabolism, nitrogen metabolism, lipid metabolism and polyphenol metabolism. It is one of the earliest reports on the metabolite identification and profiling of Chinese yam bulbils at different germination stages. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5247Chinese yam bulbilsgerminationmetabolomicsUPLC-Q-TOF-MSmultivariate statistical analysismetabolism pathway |
| spellingShingle | Xiaojin Ge Xiangyang Li Dandan Dai Zhen Yang Yanhong Wang Tiegang Yang Guixiao La Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS Acta Scientiarum Polonorum: Hortorum Cultus Chinese yam bulbils germination metabolomics UPLC-Q-TOF-MS multivariate statistical analysis metabolism pathway |
| title | Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS |
| title_full | Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS |
| title_fullStr | Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS |
| title_full_unstemmed | Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS |
| title_short | Metabolomic analysis of Chinese yam (Dioscorea polystachya Turczaninow) bulbils at different germination stages by UPLC-Q-TOF-MS |
| title_sort | metabolomic analysis of chinese yam dioscorea polystachya turczaninow bulbils at different germination stages by uplc q tof ms |
| topic | Chinese yam bulbils germination metabolomics UPLC-Q-TOF-MS multivariate statistical analysis metabolism pathway |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5247 |
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