Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays
The brown seaweed Fucus vesiculosus (Fucales, Fucaceae) was screened for its protective activity using doxorubicin-induced DNA damage in human lymphocytes. In this study, we assessed the genotoxic and antigenotoxic potential of three different concentrations (0.25, 0.5 and 1.0 mg mL-1) of F. vesicul...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2007-01-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100019 |
| _version_ | 1819002035771539456 |
|---|---|
| author | Cleide Leite-Silva Cássia Lima Silva Gusmão Catarina Satie Takahashi |
| author_facet | Cleide Leite-Silva Cássia Lima Silva Gusmão Catarina Satie Takahashi |
| author_sort | Cleide Leite-Silva |
| collection | DOAJ |
| description | The brown seaweed Fucus vesiculosus (Fucales, Fucaceae) was screened for its protective activity using doxorubicin-induced DNA damage in human lymphocytes. In this study, we assessed the genotoxic and antigenotoxic potential of three different concentrations (0.25, 0.5 and 1.0 mg mL-1) of F. vesiculosus aqueous extract using the chromosome aberration and Comet assays. Treatment of human lymphocyte cultures with 0.25, 0.5 and 1.0 mg mL-1 F. vesiculosus aqueous extract had no effect on the chromosome aberration frequency or on the extent of DNA damage detected by the Comet assay. The antigenotoxic effects of the extract were tested in human lymphocyte cultures treated with 15 µg mL-1 of doxorubicin, either alone or combined with the different concentrations of the extract, which was added to the cultures before, simultaneously with or after the doxorubicin. Only when lymphocytes were pre-treated with extract there was a reduction in doxorubicin-induced chromosome aberrations and DNA damage as detected by the Comet assay. These results demonstrate that F. vesiculosus aqueous extract is not genotoxic in cultured human lymphocytes and indicate that when added to lymphocyte cultures before doxorubicin it has antigenotoxic activity against doxorubicin-induced DNA damage. |
| first_indexed | 2024-12-20T22:58:42Z |
| format | Article |
| id | doaj.art-6e86c3e7a3c8407cbacf246725cb4fd2 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-20T22:58:42Z |
| publishDate | 2007-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-6e86c3e7a3c8407cbacf246725cb4fd22022-12-21T19:24:03ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-0130110511110.1590/S1415-47572007000100019Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assaysCleide Leite-SilvaCássia Lima Silva GusmãoCatarina Satie TakahashiThe brown seaweed Fucus vesiculosus (Fucales, Fucaceae) was screened for its protective activity using doxorubicin-induced DNA damage in human lymphocytes. In this study, we assessed the genotoxic and antigenotoxic potential of three different concentrations (0.25, 0.5 and 1.0 mg mL-1) of F. vesiculosus aqueous extract using the chromosome aberration and Comet assays. Treatment of human lymphocyte cultures with 0.25, 0.5 and 1.0 mg mL-1 F. vesiculosus aqueous extract had no effect on the chromosome aberration frequency or on the extent of DNA damage detected by the Comet assay. The antigenotoxic effects of the extract were tested in human lymphocyte cultures treated with 15 µg mL-1 of doxorubicin, either alone or combined with the different concentrations of the extract, which was added to the cultures before, simultaneously with or after the doxorubicin. Only when lymphocytes were pre-treated with extract there was a reduction in doxorubicin-induced chromosome aberrations and DNA damage as detected by the Comet assay. These results demonstrate that F. vesiculosus aqueous extract is not genotoxic in cultured human lymphocytes and indicate that when added to lymphocyte cultures before doxorubicin it has antigenotoxic activity against doxorubicin-induced DNA damage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100019antigenotoxicitychromosomal aberrationsComet assaydoxorubicinFucus vesiculosus |
| spellingShingle | Cleide Leite-Silva Cássia Lima Silva Gusmão Catarina Satie Takahashi Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays Genetics and Molecular Biology antigenotoxicity chromosomal aberrations Comet assay doxorubicin Fucus vesiculosus |
| title | Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays |
| title_full | Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays |
| title_fullStr | Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays |
| title_full_unstemmed | Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays |
| title_short | Genotoxic and antigenotoxic effects of Fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and Comet assays |
| title_sort | genotoxic and antigenotoxic effects of fucus vesiculosus extract on cultured human lymphocytes using the chromosome aberration and comet assays |
| topic | antigenotoxicity chromosomal aberrations Comet assay doxorubicin Fucus vesiculosus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100019 |
| work_keys_str_mv | AT cleideleitesilva genotoxicandantigenotoxiceffectsoffucusvesiculosusextractonculturedhumanlymphocytesusingthechromosomeaberrationandcometassays AT cassialimasilvagusmao genotoxicandantigenotoxiceffectsoffucusvesiculosusextractonculturedhumanlymphocytesusingthechromosomeaberrationandcometassays AT catarinasatietakahashi genotoxicandantigenotoxiceffectsoffucusvesiculosusextractonculturedhumanlymphocytesusingthechromosomeaberrationandcometassays |