The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization
Macrophages (Mφs) play a crucial role in the development of atherosclerosis by engulfing modified LDL particles and forming foam cells, the hallmark of atherosclerosis. Many studies suggest that myeloperoxidase-oxidized LDL (Mox-LDL) is an important pathophysiological model for LDL modification in v...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-02-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/17534259221090679 |
| _version_ | 1828366209121255424 |
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| author | Samer Bazzi Christian Frangie Eliana Azar Jalil Daher |
| author_facet | Samer Bazzi Christian Frangie Eliana Azar Jalil Daher |
| author_sort | Samer Bazzi |
| collection | DOAJ |
| description | Macrophages (Mφs) play a crucial role in the development of atherosclerosis by engulfing modified LDL particles and forming foam cells, the hallmark of atherosclerosis. Many studies suggest that myeloperoxidase-oxidized LDL (Mox-LDL) is an important pathophysiological model for LDL modification in vivo . Classically (M1) and alternatively activated (M2) Mφs are both implicated in the process of atherogenesis. Mφs are highly plastic cells whereby they undergo repolarization from M1 to M2 and vice versa . Since little is known about the effects of Mox-LDL on Mφ polarization and repolarization, our study aimed at evaluating the in vitro effects of Mox-LDL at this level through making use of the well-established model of human THP-1-derived Mφs. Resting M0-Mφs were polarized toward M1- and M2-Mφs, then M0-, M1- and M2-Mφs were all treated with physiological concentrations of Mox-LDL to assess the effect of Mox-LDL treatment on Mφ polarization and repolarization. Treatment of M0-Mφs with a physiological concentration of Mox-LDL had no significant effects at the level of their polarization. However, treatment of M1-Mφs with Mox-LDL resulted in a significant reduction in their IL-10 cytokine secretion. Our results point to a potential role of Mox-LDL in increasing the pro-inflammatory state in Mφs through reducing the release of the anti-inflammatory cytokine, IL-10. |
| first_indexed | 2024-04-14T05:42:08Z |
| format | Article |
| id | doaj.art-6e891d4baa104d9f9ba0ea6b33fe205e |
| institution | Directory Open Access Journal |
| issn | 1753-4259 1753-4267 |
| language | English |
| last_indexed | 2024-04-14T05:42:08Z |
| publishDate | 2022-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj.art-6e891d4baa104d9f9ba0ea6b33fe205e2022-12-22T02:09:25ZengSAGE PublishingInnate Immunity1753-42591753-42672022-02-012810.1177/17534259221090679The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarizationSamer BazziChristian FrangieEliana AzarJalil DaherMacrophages (Mφs) play a crucial role in the development of atherosclerosis by engulfing modified LDL particles and forming foam cells, the hallmark of atherosclerosis. Many studies suggest that myeloperoxidase-oxidized LDL (Mox-LDL) is an important pathophysiological model for LDL modification in vivo . Classically (M1) and alternatively activated (M2) Mφs are both implicated in the process of atherogenesis. Mφs are highly plastic cells whereby they undergo repolarization from M1 to M2 and vice versa . Since little is known about the effects of Mox-LDL on Mφ polarization and repolarization, our study aimed at evaluating the in vitro effects of Mox-LDL at this level through making use of the well-established model of human THP-1-derived Mφs. Resting M0-Mφs were polarized toward M1- and M2-Mφs, then M0-, M1- and M2-Mφs were all treated with physiological concentrations of Mox-LDL to assess the effect of Mox-LDL treatment on Mφ polarization and repolarization. Treatment of M0-Mφs with a physiological concentration of Mox-LDL had no significant effects at the level of their polarization. However, treatment of M1-Mφs with Mox-LDL resulted in a significant reduction in their IL-10 cytokine secretion. Our results point to a potential role of Mox-LDL in increasing the pro-inflammatory state in Mφs through reducing the release of the anti-inflammatory cytokine, IL-10.https://doi.org/10.1177/17534259221090679 |
| spellingShingle | Samer Bazzi Christian Frangie Eliana Azar Jalil Daher The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization Innate Immunity |
| title | The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization |
| title_full | The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization |
| title_fullStr | The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization |
| title_full_unstemmed | The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization |
| title_short | The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization |
| title_sort | effect of myeloperoxidase oxidized ldl on thp 1 macrophage polarization and repolarization |
| url | https://doi.org/10.1177/17534259221090679 |
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