Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells
Hypoxia is a common factor of numerous ocular diseases that lead to dysfunctions and loss of retinal ganglion cells (RGCs) with subsequent vision loss. High-voltage-activated calcium channels are the main source of calcium entry into neurons. Their activity plays a central role in different signalin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2023.1202083/full |
| _version_ | 1797789148056125440 |
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| author | Hanna Dumanska Mariia Telka Nikolai Veselovsky |
| author_facet | Hanna Dumanska Mariia Telka Nikolai Veselovsky |
| author_sort | Hanna Dumanska |
| collection | DOAJ |
| description | Hypoxia is a common factor of numerous ocular diseases that lead to dysfunctions and loss of retinal ganglion cells (RGCs) with subsequent vision loss. High-voltage-activated calcium channels are the main source of calcium entry into neurons. Their activity plays a central role in different signaling processes in health and diseases, such as enzyme activation, gene transcription, synaptic transmission, or the onset of cell death. This study aims to establish and evaluate the initial effect of the early stage of acute hypoxia on somatic HVA calcium currents in cultured RGCs. HVA calcium currents were recorded in RGCs using the whole-cell patch-clamp technique in the voltage-clamp mode. The fast local superfusion was used for a brief (up to 270 s) application of the hypoxic solution (pO2 < 5 mmHg). The switch from normoxic to hypoxic solutions and vice versa was less than 1 s. The HVA calcium channel activity was inhibited by acute hypoxia in 79% of RGCs (30 of 38 RGCs) in a strong voltage-dependent manner. The level of inhibition was independent of the duration of hypoxia or repeated applications. The hypoxia-induced inhibition of calcium currents had a strong correlation with the duration of hypoxia and showed the transition from reversible to irreversible at 75 s of hypoxia and longer. The results obtained are the first demonstration of the phenomena of HVA calcium current inhibition by acute hypoxia in RGCs and provide a conceptual framework for further research. |
| first_indexed | 2024-03-13T01:46:37Z |
| format | Article |
| id | doaj.art-6e896b8898cb4b52b19ee8cc8d722df2 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-03-13T01:46:37Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-6e896b8898cb4b52b19ee8cc8d722df22023-07-03T07:24:40ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022023-07-011710.3389/fncel.2023.12020831202083Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cellsHanna DumanskaMariia TelkaNikolai VeselovskyHypoxia is a common factor of numerous ocular diseases that lead to dysfunctions and loss of retinal ganglion cells (RGCs) with subsequent vision loss. High-voltage-activated calcium channels are the main source of calcium entry into neurons. Their activity plays a central role in different signaling processes in health and diseases, such as enzyme activation, gene transcription, synaptic transmission, or the onset of cell death. This study aims to establish and evaluate the initial effect of the early stage of acute hypoxia on somatic HVA calcium currents in cultured RGCs. HVA calcium currents were recorded in RGCs using the whole-cell patch-clamp technique in the voltage-clamp mode. The fast local superfusion was used for a brief (up to 270 s) application of the hypoxic solution (pO2 < 5 mmHg). The switch from normoxic to hypoxic solutions and vice versa was less than 1 s. The HVA calcium channel activity was inhibited by acute hypoxia in 79% of RGCs (30 of 38 RGCs) in a strong voltage-dependent manner. The level of inhibition was independent of the duration of hypoxia or repeated applications. The hypoxia-induced inhibition of calcium currents had a strong correlation with the duration of hypoxia and showed the transition from reversible to irreversible at 75 s of hypoxia and longer. The results obtained are the first demonstration of the phenomena of HVA calcium current inhibition by acute hypoxia in RGCs and provide a conceptual framework for further research.https://www.frontiersin.org/articles/10.3389/fncel.2023.1202083/fullretinal ganglion cellssomatic calcium channelshigh-voltage-activated calcium currentsacute hypoxiareversible and irreversible inhibition |
| spellingShingle | Hanna Dumanska Mariia Telka Nikolai Veselovsky Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells Frontiers in Cellular Neuroscience retinal ganglion cells somatic calcium channels high-voltage-activated calcium currents acute hypoxia reversible and irreversible inhibition |
| title | Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells |
| title_full | Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells |
| title_fullStr | Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells |
| title_full_unstemmed | Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells |
| title_short | Inhibition of high-voltage-activated calcium currents by acute hypoxia in cultured retinal ganglion cells |
| title_sort | inhibition of high voltage activated calcium currents by acute hypoxia in cultured retinal ganglion cells |
| topic | retinal ganglion cells somatic calcium channels high-voltage-activated calcium currents acute hypoxia reversible and irreversible inhibition |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2023.1202083/full |
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