A modifiable microarray-based universal sensor: providing sample-to-results automation
A microfluidic system consisting of generic single use cartridges which interface with a workstation allows the automatic performance of all necessary sample preparation, PCR analysis and interpretation of multiplex PCR assays. The cartridges contain a DNA array with 20 different 16mer DNA “universa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844016310143 |
| _version_ | 1828389989256265728 |
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| author | Rubina Yasmin Hui Zhu Zongyuan Chen Richard A. Montagna |
| author_facet | Rubina Yasmin Hui Zhu Zongyuan Chen Richard A. Montagna |
| author_sort | Rubina Yasmin |
| collection | DOAJ |
| description | A microfluidic system consisting of generic single use cartridges which interface with a workstation allows the automatic performance of all necessary sample preparation, PCR analysis and interpretation of multiplex PCR assays. The cartridges contain a DNA array with 20 different 16mer DNA “universal” probes immobilized at defined locations. PCR amplicons can be detected via hybridization of user-defined “reporter” probes that are complementary at their 3′ termini to one or more of the universal probes and complementary to the target amplicons at their 5′ termini. The system was able to detect single-plex and multiplex PCR amplicons from various infectious agents as well as wild type and mutant alleles of single nucleotide polymorphisms. The system's ease of use was further demonstrated by converting a published PCR assay for the detection of Mycobacterium genitalium in a fully automated manner. Excellent correlation between traditional manual methods and the automated analysis performed by the workstation suggests that the system can provide a means to easily design and implement a variety of customized PCR-based assays. The system will be useful to researchers or clinical investigators seeking to develop their own user defined assays. As the U.S. FDA continues to pursue regulatory oversight of LDTs, the system would also allow labs to continue to develop compliant assays. |
| first_indexed | 2024-12-10T06:36:37Z |
| format | Article |
| id | doaj.art-6e8975afe0d34253b97315471a9ee700 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T06:36:37Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-6e8975afe0d34253b97315471a9ee7002022-12-22T01:58:55ZengElsevierHeliyon2405-84402016-10-01210e00179A modifiable microarray-based universal sensor: providing sample-to-results automationRubina Yasmin0Hui Zhu1Zongyuan Chen2Richard A. Montagna3Rheonix Inc., 10 Brown Road, Ithaca, NY 14850, USARheonix Inc., 10 Brown Road, Ithaca, NY 14850, USARheonix Inc., 10 Brown Road, Ithaca, NY 14850, USA; Thermo Fisher Scientific, South San Francisco, CA, USARheonix Inc., 10 Brown Road, Ithaca, NY 14850, USA; Corresponding author.A microfluidic system consisting of generic single use cartridges which interface with a workstation allows the automatic performance of all necessary sample preparation, PCR analysis and interpretation of multiplex PCR assays. The cartridges contain a DNA array with 20 different 16mer DNA “universal” probes immobilized at defined locations. PCR amplicons can be detected via hybridization of user-defined “reporter” probes that are complementary at their 3′ termini to one or more of the universal probes and complementary to the target amplicons at their 5′ termini. The system was able to detect single-plex and multiplex PCR amplicons from various infectious agents as well as wild type and mutant alleles of single nucleotide polymorphisms. The system's ease of use was further demonstrated by converting a published PCR assay for the detection of Mycobacterium genitalium in a fully automated manner. Excellent correlation between traditional manual methods and the automated analysis performed by the workstation suggests that the system can provide a means to easily design and implement a variety of customized PCR-based assays. The system will be useful to researchers or clinical investigators seeking to develop their own user defined assays. As the U.S. FDA continues to pursue regulatory oversight of LDTs, the system would also allow labs to continue to develop compliant assays.http://www.sciencedirect.com/science/article/pii/S2405844016310143BioengineeringBiotechnology |
| spellingShingle | Rubina Yasmin Hui Zhu Zongyuan Chen Richard A. Montagna A modifiable microarray-based universal sensor: providing sample-to-results automation Heliyon Bioengineering Biotechnology |
| title | A modifiable microarray-based universal sensor: providing sample-to-results automation |
| title_full | A modifiable microarray-based universal sensor: providing sample-to-results automation |
| title_fullStr | A modifiable microarray-based universal sensor: providing sample-to-results automation |
| title_full_unstemmed | A modifiable microarray-based universal sensor: providing sample-to-results automation |
| title_short | A modifiable microarray-based universal sensor: providing sample-to-results automation |
| title_sort | modifiable microarray based universal sensor providing sample to results automation |
| topic | Bioengineering Biotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844016310143 |
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