Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition
Cytoglobin (Cygb) is a hexacoordinate protein, associated with the transport of oxygen, nitric oxide scavenging, tumor suppression and protection against oxidative stress and inflammation. This protein is expressed in brain areas including the preoptic area (POA) of the anterior hypothalamus, the re...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnmol.2019.00307/full |
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| author | Bruna R. B. Gomes Gabriela Luna S. de Sousa Daniela Ott Jolanta Murgott Marcelo V. de Sousa Paulo E. N. de Souza Joachim Roth Fabiane H. Veiga-Souza Fabiane H. Veiga-Souza |
| author_facet | Bruna R. B. Gomes Gabriela Luna S. de Sousa Daniela Ott Jolanta Murgott Marcelo V. de Sousa Paulo E. N. de Souza Joachim Roth Fabiane H. Veiga-Souza Fabiane H. Veiga-Souza |
| author_sort | Bruna R. B. Gomes |
| collection | DOAJ |
| description | Cytoglobin (Cygb) is a hexacoordinate protein, associated with the transport of oxygen, nitric oxide scavenging, tumor suppression and protection against oxidative stress and inflammation. This protein is expressed in brain areas including the preoptic area (POA) of the anterior hypothalamus, the region responsible for the regulation of body temperature. In this study, we show that Cygb is upregulated in the rat hypothalamus 2.5 h and 5 h after intravenous administration of lipopolysaccharide (LPS). We investigated the effect of treatment with Cygb in POA primary cultures stimulated with LPS for 4 h. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured and the results showed that Cygb reduced the concentrations of both cytokines. We further observed a decrease in immunoreactivity of the inflammatory transcription factor nuclear factor-κB (NF-κB), but not NF-IL6 and STAT3, in the nucleus of Cygb-treated POA cells. These findings suggest that Cygb attenuates the secretion of IL-6 and TNF-α in LPS-stimulated POA primary cultures via inhibition of the NF-κB signaling pathway, indicating that this protein might play an important role in the control of neuroinflammation and fever. |
| first_indexed | 2024-12-11T09:16:05Z |
| format | Article |
| id | doaj.art-6e8b824c57154a4dba2d876fda20bef8 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-11T09:16:05Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-6e8b824c57154a4dba2d876fda20bef82022-12-22T01:13:22ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992019-12-011210.3389/fnmol.2019.00307485400Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway InhibitionBruna R. B. Gomes0Gabriela Luna S. de Sousa1Daniela Ott2Jolanta Murgott3Marcelo V. de Sousa4Paulo E. N. de Souza5Joachim Roth6Fabiane H. Veiga-Souza7Fabiane H. Veiga-Souza8Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, BrazilSchool of Ceilandia, University of Brasília, Brasília, BrazilVeterinary Physiology, Faculty of Veterinary Medicine, Justus-Liebig-University of Giessen, Giessen, GermanyVeterinary Physiology, Faculty of Veterinary Medicine, Justus-Liebig-University of Giessen, Giessen, GermanyLaboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, BrazilLaboratory of Electron Paramagnetic Resonance, Institute of Physics, University of Brasília, Brasília, BrazilVeterinary Physiology, Faculty of Veterinary Medicine, Justus-Liebig-University of Giessen, Giessen, GermanyLaboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, BrazilSchool of Ceilandia, University of Brasília, Brasília, BrazilCytoglobin (Cygb) is a hexacoordinate protein, associated with the transport of oxygen, nitric oxide scavenging, tumor suppression and protection against oxidative stress and inflammation. This protein is expressed in brain areas including the preoptic area (POA) of the anterior hypothalamus, the region responsible for the regulation of body temperature. In this study, we show that Cygb is upregulated in the rat hypothalamus 2.5 h and 5 h after intravenous administration of lipopolysaccharide (LPS). We investigated the effect of treatment with Cygb in POA primary cultures stimulated with LPS for 4 h. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured and the results showed that Cygb reduced the concentrations of both cytokines. We further observed a decrease in immunoreactivity of the inflammatory transcription factor nuclear factor-κB (NF-κB), but not NF-IL6 and STAT3, in the nucleus of Cygb-treated POA cells. These findings suggest that Cygb attenuates the secretion of IL-6 and TNF-α in LPS-stimulated POA primary cultures via inhibition of the NF-κB signaling pathway, indicating that this protein might play an important role in the control of neuroinflammation and fever.https://www.frontiersin.org/article/10.3389/fnmol.2019.00307/fullcytoglobinneuroinflammationfeverpreoptic areaprimary culturehypothalamus |
| spellingShingle | Bruna R. B. Gomes Gabriela Luna S. de Sousa Daniela Ott Jolanta Murgott Marcelo V. de Sousa Paulo E. N. de Souza Joachim Roth Fabiane H. Veiga-Souza Fabiane H. Veiga-Souza Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition Frontiers in Molecular Neuroscience cytoglobin neuroinflammation fever preoptic area primary culture hypothalamus |
| title | Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition |
| title_full | Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition |
| title_fullStr | Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition |
| title_full_unstemmed | Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition |
| title_short | Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition |
| title_sort | cytoglobin attenuates neuroinflammation in lipopolysaccharide activated primary preoptic area cells via nf κb pathway inhibition |
| topic | cytoglobin neuroinflammation fever preoptic area primary culture hypothalamus |
| url | https://www.frontiersin.org/article/10.3389/fnmol.2019.00307/full |
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